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1.发明授权Adenoviral vectors capable of facilitating increased persistence of transgene expression 失效能够促进转基因表达持久性增强的腺病毒载体
公开(公告)号：US6020191A
公开(公告)日：2000-02-01
申请号：US839553
申请日：1997-04-14
CPC分类号： C12N15/86 , C07K14/4712 , A61K48/00 , C12N2710/10343
摘要： The invention is directed to novel adenoviral vectors which are capable of facilitating persistent expression of a transgene which is delivered by the vector to a cell. The vectors are E1/partial E3 deleted vectors which contain a transgene operably linked to expression control sequences, preferably the CMV promoter. The invention is also directed to compositions comprising the adenoviral vectors of the invention and to methods for providing persistent expression of a transgene to the cells of an individual by administration of the compositions.
摘要翻译： 本发明涉及新颖的腺病毒载体，其能够促进由载体递送至细胞的转基因的持续表达。 载体是含有可操作地连接到表达调控序列，优选CMV启动子的转基因的E1 /部分E3缺失载体。 本发明还涉及包含本发明的腺病毒载体的组合物以及通过施用组合物来提供转基因对个体细胞的持续表达的方法。
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公开(公告)号：US20090082289A1
公开(公告)日：2009-03-26
申请号：US11800036
申请日：2007-05-02
CPC分类号： C12N15/86 , A61K48/00 , C12N2710/10332 , C12N2710/10343 , C12N2830/008 , C12N2830/85
摘要： This invention provides a recombinant adenovirus expression vector characterized by the partial or total deletion of the adenoviral protein IX DNA and having a gene encoding a foreign protein or a functional fragment or mutant thereof. Transformed host cells and a method of producing recombinant proteins and gene therapy also are included within the scope of this invention.Thus, for example, the adenoviral vector of this invention- can contain a foreign gene for the expression of a protein effective in regulating the cell cycle, such as p53, Rb, or mitosin, or in inducing cell death, such as the conditional suicide gene thymidine kinase. (The latter must be used in conjunction with a thymidine kinase metabolite in order to be effective).
摘要翻译： 本发明提供一种重组腺病毒表达载体，其特征在于部分或完全缺失腺病毒蛋白IX DNA并具有编码外来蛋白或其功能片段或突变体的基因。 转化的宿主细胞和生产重组蛋白和基因治疗的方法也包括在本发明的范围内。 因此，例如，本发明的腺病毒载体可以含有外源基因，用于表达有效调节细胞周期的蛋白质，例如p53，Rb或mitosin，或诱导细胞死亡，例如有条件的自杀 基因胸苷激酶。 （后者必须与胸苷激酶代谢物结合使用才能有效）。
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公开(公告)号：US06432927B1
公开(公告)日：2002-08-13
申请号：US09579897
申请日：2000-05-26
申请人： Richard J. Gregory , Karen Vincent
发明人： Richard J. Gregory , Karen Vincent
IPC分类号： A61K3170
CPC分类号： C12N15/85 , A61K38/00 , A61K48/00 , C07K14/4702 , C07K14/52 , C07K2319/00 , C07K2319/71 , C07K2319/80 , C12N2799/022
摘要： The present invention provides recombinant nucleic acid molecules encoding a chimeric transactivator protein including a DNA binding domain of a DNA binding protein and a protein domain capable of transcriptional activation. The present invention also provides recombinant viral and non-viral vectors that are able to infect and/or transfect and sustain expression of a biologically active chimeric transactivator proteins in mammalian cells. Also provided are host cell lines and non-human transgenic animals capable of expressing biologically active chimeric transactivator proteins. In another aspect, compositions and methods for treating or preventing ischemic damage associated with hypoxia-related disorders are provided.
摘要翻译： 本发明提供了编码嵌合反式激活因子蛋白的重组核酸分子，其包括DNA结合蛋白的DNA结合结构域和能够转录激活的蛋白质结构域。 本发明还提供能够感染和/或转染和维持哺乳动物细胞中生物活性嵌合反式激活因子蛋白表达的重组病毒和非病毒载体。 还提供了能够表达生物活性嵌合反式激活蛋白的宿主细胞系和非人转基因动物。 在另一方面，提供了用于治疗或预防与缺氧相关疾病相关的缺血性损伤的组合物和方法。
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公开(公告)号：US06358507B1
公开(公告)日：2002-03-19
申请号：US09416673
申请日：1999-10-12
IPC分类号： A61K4800
CPC分类号： C12N15/86 , A61K38/1709 , A61K48/00 , C12N2710/10322 , C12N2710/10343
摘要： The present invention relates to transgene expression systems, related compositions comprising the transgene expression systems, and methods of making and using them. Preferred systems employ an adenovirus transgene expression vector comprising DNA encoding a transgene which codes for a desired product operably linked to expression control sequence, and at least a portion of the adenovirus E3 region and certain portions of the E4 region. The E4 portions comprise the open reading frame sequence known as E40RF3 and at least one other portion of E4. Preferably the E4 portion of the vector (or “E4 cassette”) includes E40RF3 and at least one other portion selected from E40RF4, E40RF6/7 and E40RF3/4. The invention has a number of important features including improving persistency of transgene expression in a desired host cell. The transgene expression systems of the present invention are useful for a variety of applications including providing persistent cellular expression of the transgene in vitro and in vivo.
摘要翻译： 本发明涉及转基因表达系统，包含转基因表达系统的相关组合物，以及制备和使用它们的方法。 优选的系统使用腺病毒转基因表达载体，其包含编码转基因的DNA，所述转基因编码与表达控制序列可操作地连接的所需产物，以及腺病毒E3区域的至少一部分和E4区域的某些部分。 E4部分包括被称为E40RF3的开放阅读框序列和E4的至少一个其他部分。 优选地，载体（或“E4盒”）的E4部分包括E40RF3和至少一个选自E40RF4，E40RF6 / 7和E40RF3 / 4的其它部分。 本发明具有许多重要特征，包括改善所需宿主细胞中转基因表达的持续性。 本发明的转基因表达系统可用于各种应用，包括在体外和体内提供转基因的持续细胞表达。
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公开(公告)号：US20080299083A1
公开(公告)日：2008-12-04
申请号：US11818907
申请日：2007-06-14
CPC分类号： C12N15/86 , A61K48/00 , C12N2710/10332 , C12N2710/10343 , C12N2830/008 , C12N2830/85
摘要： This invention provides a recombinant adenovirus expression vector characterized by the partial or total deletion of the adenoviral protein IX DNA and having a gene encoding a foreign protein or a functional fragment or mutant thereof. Transformed host cells and a method of producing recombinant proteins and gene therapy also are included within the scope of this invention.Thus, for example, the adenoviral vector of this invention can contain a foreign gene for the expression of a protein effective in regulating the cell cycle, such as p53, Rb, or mitosin, or in inducing cell death, such as the conditional suicide gene thymidine kinase. (The latter must be used in conjunction with a thymidine kinase metabolite in order to be effective).
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公开(公告)号：US06485720B2
公开(公告)日：2002-11-26
申请号：US09924925
申请日：2001-08-08
IPC分类号： A61K4800
CPC分类号： C12N15/86 , A61K38/1709 , A61K48/00 , C12N2710/10322 , C12N2710/10343
摘要： The present invention relates to transgene expression systems, related compositions comprising the transgene expression systems, and methods of making and using them. Preferred systems employ an adenovirus transgene expression vector comprising DNA encoding a transgene which codes for a desired product operably linked to expression control sequence, and at least a portion of the adenovirus E3 region and certain portions of the E4 region. The E4 portions comprise the open reading frame sequence known as E4ORF3 and at least one other portion of E4. Preferably the E4 portion of the vector (or “E4 cassette”) includes E4ORF3 and at least one other portion selected from E4ORF4, E4ORF6/7 and E4ORF3/4. The invention has a number of important features including improving persistency of transgene expression in a desired host cell. The transgene expression systems of the present invention are useful for a variety of applications including providing persistent cellular expression of the transgene in vitro and in vivo.
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公开(公告)号：US06379927B1
公开(公告)日：2002-04-30
申请号：US09315113
申请日：1999-05-19
IPC分类号： C12P2104
CPC分类号： C07K14/4736 , A61K48/00 , C07K2319/00 , C12N2799/022
摘要： Fusions of the transcription factor E2F and the retinoblastoma protein RB are provided, along with methods of treatment of hyperproliferative diseases.
摘要翻译： 提供转录因子E2F和视网膜母细胞瘤蛋白RB的融合以及过度增殖性疾病的治疗方法。
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公开(公告)号：US06210939B1
公开(公告)日：2001-04-03
申请号：US08328673
申请日：1994-10-25
IPC分类号： C12N510
CPC分类号： C12N15/86 , A61K38/00 , C07K14/4746 , C12N2710/10343 , C12N2830/008 , C12N2830/85 , Y02A50/473
摘要： This invention provides a recombinant adenovirus expression vector characterized by the partial or total deletion of the adenoviral protein IX DNA and having a gene encoding a foreign protein or a functional fragment or mutant thereof. Transformed host cells and a method of producing recombinant proteins and gene therapy also are included within the scope of this invention. Thus, for example, the adenoviral vector of this invention can contain a foreign gene for the expression of a protein effective in regulating the cell cycle, such as p53, Rb, or mitosin, or in inducing cell death, such as the conditional suicide gene thymidine kinase. (The latter must be used in conjunction with a thymidine kinase metabolite in order to be effective).
摘要翻译： 本发明提供一种重组腺病毒表达载体，其特征在于部分或完全缺失腺病毒蛋白IX DNA并具有编码外来蛋白或其功能片段或突变体的基因。 转化的宿主细胞和生产重组蛋白和基因治疗的方法也包括在本发明的范围内。 因此，例如，本发明的腺病毒载体可以含有外源基因，用于表达有效调节细胞周期的蛋白质，如p53，Rb或mitosin，或诱导细胞死亡，如条件性自杀基因 胸苷激酶。 （后者必须与胸苷激酶代谢物结合使用才能有效）。
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公开(公告)号：US6093567A
公开(公告)日：2000-07-25
申请号：US248026
申请日：1999-02-10
IPC分类号： A61K38/00 , A61K48/00 , C07K14/47 , C12N15/861
CPC分类号： C12N15/86 , A61K48/00 , C07K14/4712 , A61K38/00 , C12N2710/10343 , C12N2830/42 , C12N2840/203
摘要： Gene Therapy vectors, which are especially useful for cystic fibrosis, and methods for using the vectors are disclosed.
摘要翻译： 公开了对囊性纤维化特别有用的基因治疗载体和使用载体的方法。
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公开(公告)号：US07053062B2
公开(公告)日：2006-05-30
申请号：US10190394
申请日：2002-07-03
申请人： Richard J. Gregory , Karen Vincent
发明人： Richard J. Gregory , Karen Vincent
CPC分类号： C12N15/85 , A61K38/00 , A61K48/00 , C07K14/4702 , C07K14/52 , C07K2319/00 , C07K2319/71 , C07K2319/80 , C12N2799/022
摘要： The present invention provides recombinant nucleic acid molecules encoding a chimeric transactivator protein including a DNA binding domain of a DNA binding protein and a protein domain capable of transcriptional activation. The present invention also provides recombinant viral and non-viral vectors that are able to infect and/or transfect and sustain expression of a biologically active chimeric transactivator proteins in mammalian cells. Also provided are host cell lines and non-human transgenic animals capable of expressing biologically active chimeric transactivator proteins. In another aspect, compositions and methods for treating or preventing ischemic damage associated with hypoxia-related disorders are provided.
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