-
公开(公告)号：US20090082289A1
公开(公告)日：2009-03-26
申请号：US11800036
申请日：2007-05-02
CPC分类号： C12N15/86 , A61K48/00 , C12N2710/10332 , C12N2710/10343 , C12N2830/008 , C12N2830/85
摘要： This invention provides a recombinant adenovirus expression vector characterized by the partial or total deletion of the adenoviral protein IX DNA and having a gene encoding a foreign protein or a functional fragment or mutant thereof. Transformed host cells and a method of producing recombinant proteins and gene therapy also are included within the scope of this invention.Thus, for example, the adenoviral vector of this invention- can contain a foreign gene for the expression of a protein effective in regulating the cell cycle, such as p53, Rb, or mitosin, or in inducing cell death, such as the conditional suicide gene thymidine kinase. (The latter must be used in conjunction with a thymidine kinase metabolite in order to be effective).
摘要翻译： 本发明提供一种重组腺病毒表达载体，其特征在于部分或完全缺失腺病毒蛋白IX DNA并具有编码外来蛋白或其功能片段或突变体的基因。 转化的宿主细胞和生产重组蛋白和基因治疗的方法也包括在本发明的范围内。 因此，例如，本发明的腺病毒载体可以含有外源基因，用于表达有效调节细胞周期的蛋白质，例如p53，Rb或mitosin，或诱导细胞死亡，例如有条件的自杀 基因胸苷激酶。 （后者必须与胸苷激酶代谢物结合使用才能有效）。
-
公开(公告)号：US06432927B1
公开(公告)日：2002-08-13
申请号：US09579897
申请日：2000-05-26
申请人： Richard J. Gregory , Karen Vincent
发明人： Richard J. Gregory , Karen Vincent
IPC分类号： A61K3170
CPC分类号： C12N15/85 , A61K38/00 , A61K48/00 , C07K14/4702 , C07K14/52 , C07K2319/00 , C07K2319/71 , C07K2319/80 , C12N2799/022
摘要： The present invention provides recombinant nucleic acid molecules encoding a chimeric transactivator protein including a DNA binding domain of a DNA binding protein and a protein domain capable of transcriptional activation. The present invention also provides recombinant viral and non-viral vectors that are able to infect and/or transfect and sustain expression of a biologically active chimeric transactivator proteins in mammalian cells. Also provided are host cell lines and non-human transgenic animals capable of expressing biologically active chimeric transactivator proteins. In another aspect, compositions and methods for treating or preventing ischemic damage associated with hypoxia-related disorders are provided.
摘要翻译： 本发明提供了编码嵌合反式激活因子蛋白的重组核酸分子，其包括DNA结合蛋白的DNA结合结构域和能够转录激活的蛋白质结构域。 本发明还提供能够感染和/或转染和维持哺乳动物细胞中生物活性嵌合反式激活因子蛋白表达的重组病毒和非病毒载体。 还提供了能够表达生物活性嵌合反式激活蛋白的宿主细胞系和非人转基因动物。 在另一方面，提供了用于治疗或预防与缺氧相关疾病相关的缺血性损伤的组合物和方法。
-
公开(公告)号：US06358507B1
公开(公告)日：2002-03-19
申请号：US09416673
申请日：1999-10-12
IPC分类号： A61K4800
CPC分类号： C12N15/86 , A61K38/1709 , A61K48/00 , C12N2710/10322 , C12N2710/10343
摘要： The present invention relates to transgene expression systems, related compositions comprising the transgene expression systems, and methods of making and using them. Preferred systems employ an adenovirus transgene expression vector comprising DNA encoding a transgene which codes for a desired product operably linked to expression control sequence, and at least a portion of the adenovirus E3 region and certain portions of the E4 region. The E4 portions comprise the open reading frame sequence known as E40RF3 and at least one other portion of E4. Preferably the E4 portion of the vector (or “E4 cassette”) includes E40RF3 and at least one other portion selected from E40RF4, E40RF6/7 and E40RF3/4. The invention has a number of important features including improving persistency of transgene expression in a desired host cell. The transgene expression systems of the present invention are useful for a variety of applications including providing persistent cellular expression of the transgene in vitro and in vivo.
摘要翻译： 本发明涉及转基因表达系统，包含转基因表达系统的相关组合物，以及制备和使用它们的方法。 优选的系统使用腺病毒转基因表达载体，其包含编码转基因的DNA，所述转基因编码与表达控制序列可操作地连接的所需产物，以及腺病毒E3区域的至少一部分和E4区域的某些部分。 E4部分包括被称为E40RF3的开放阅读框序列和E4的至少一个其他部分。 优选地，载体（或“E4盒”）的E4部分包括E40RF3和至少一个选自E40RF4，E40RF6 / 7和E40RF3 / 4的其它部分。 本发明具有许多重要特征，包括改善所需宿主细胞中转基因表达的持续性。 本发明的转基因表达系统可用于各种应用，包括在体外和体内提供转基因的持续细胞表达。
-
公开(公告)号：US20090088398A1
公开(公告)日：2009-04-02
申请号：US12127756
申请日：2008-05-27
IPC分类号： A61K31/7088 , C12N15/00 , C12N5/06 , C12N1/21 , C12N15/87
CPC分类号： C12N15/86 , A61K48/00 , C12N2710/10332 , C12N2710/10343 , C12N2830/008 , C12N2830/85
摘要： This invention provides a recombinant adenovirus expression vector characterized by the partial or total deletion of the adenoviral protein IX DNA and having a gene encoding a foreign protein or a functional fragment or mutant thereof. Transformed host cells and a method of producing recombinant proteins and gene therapy also are included within the scope of this invention. Thus, for example, the adenoviral vector of this invention can contain a foreign gene for the expression of a protein effective in regulating the cell cycle, such as p53, Rb, or mitosin, or in inducing cell death, such as the conditional suicide gene thymidine kinase. (The latter must be used in conjunction with a thymidine kinase metabolite in order to be effective.)
摘要翻译： 本发明提供一种重组腺病毒表达载体，其特征在于部分或完全缺失腺病毒蛋白IX DNA并具有编码外来蛋白或其功能片段或突变体的基因。 转化的宿主细胞和生产重组蛋白和基因治疗的方法也包括在本发明的范围内。 因此，例如，本发明的腺病毒载体可以含有外源基因，用于表达有效调节细胞周期的蛋白质，如p53，Rb或mitosin，或诱导细胞死亡，如条件性自杀基因 胸苷激酶。 （后者必须与胸苷激酶代谢物结合使用才能有效）
-
公开(公告)号：US07041284B2
公开(公告)日：2006-05-09
申请号：US08958570
申请日：1997-10-28
IPC分类号： A61K48/00 , C12N15/861 , C12N15/63
CPC分类号： C12N15/86 , A61K38/00 , C07K14/4746 , C12N2710/10343 , C12N2830/008 , C12N2830/85 , Y02A50/473
摘要： This invention provides a recombinant adenovirus expression vector characterized by the partial or total deletion of the adenoviral protein IX DNA and having a gene encoding a foreign protein or a functional fragment or mutant thereof. Transformed host cells and a method of producing recombinant proteins and gene therapy also are included within the scope of this invention. Thus, for example, the adenoviral vector of this invention can contain a foreign gene for the expression of a protein effective in regulating the cell cycle, such as p53, Rb, or mitosin, or in inducing cell death, such as the conditional suicide gene thymidine dinase. (The latter must be used in conjunction with a thymidine kinase metabolite in order to be effective.)
摘要翻译： 本发明提供一种重组腺病毒表达载体，其特征在于部分或完全缺失腺病毒蛋白IX DNA并具有编码外来蛋白或其功能片段或突变体的基因。 转化的宿主细胞和生产重组蛋白和基因治疗的方法也包括在本发明的范围内。 因此，例如，本发明的腺病毒载体可以含有外源基因，用于表达有效调节细胞周期的蛋白质，如p53，Rb或mitosin，或诱导细胞死亡，如条件性自杀基因 胸苷酶。 （后者必须与胸苷激酶代谢物结合使用才能有效）
-
6.发明授权Methods of treating hyperproliferative disorders using retinoblastoma fusion proteins 失效使用视网膜母细胞瘤融合蛋白治疗过度增殖性疾病的方法公开(公告)号：US06902731B1
公开(公告)日：2005-06-07
申请号：US09315116
申请日：1999-05-19
IPC分类号： C12N15/09 , A61K31/7088 , A61K48/00 , A61P5/00 , A61P13/08 , A61P17/06 , A61P27/02 , A61P35/00 , A61P43/00 , C07K14/47 , C07K14/82 , C07K19/00 , A01N63/00 , A01N43/04 , C12N15/00 , C12N15/63
CPC分类号： C07K14/4736 , A61K48/00 , C07K2319/00 , C12N2799/022
摘要： Fusions of the transcription factor E2F and the retinoblastoma protein RB are provided, along with methods of treatment of hyperproliferative diseases.
摘要翻译： 提供转录因子E2F和视网膜母细胞瘤蛋白RB的融合以及过度增殖性疾病的治疗方法。
-
7.发明授权Organ-specific targeting of cationic amphiphile / DNA complexes for gene therapy 失效用于基因治疗的阳离子两亲物/ DNA复合物的器官特异性靶向公开(公告)号：US06331524B1
公开(公告)日：2001-12-18
申请号：US08835213
申请日：1997-04-07
申请人： Ronald K. Scheule , Rebecca G. Bagley , Simon J. Eastman , Seng H. Cheng , John Marshall , David J. Harris , Edward R. Lee , Craig S. Siegel , Chau-Dung Chang , S. Catherine Hubbard , Duane E. Johnson , Daniel C. Maneval , H. Michael Shepard , Richard J. Gregory
发明人： Ronald K. Scheule , Rebecca G. Bagley , Simon J. Eastman , Seng H. Cheng , John Marshall , David J. Harris , Edward R. Lee , Craig S. Siegel , Chau-Dung Chang , S. Catherine Hubbard , Duane E. Johnson , Daniel C. Maneval , H. Michael Shepard , Richard J. Gregory
IPC分类号： A61K4800
CPC分类号： C07J41/0055 , A61K9/1272 , A61K47/54 , A61K47/542 , A61K47/543 , A61K47/554 , A61K48/00 , C07J41/0005 , C07J41/0061 , C07J43/003 , C12N15/88
摘要： Novel cationic amphiphiles are provided that facilitate transport of biologically active (therapeutic) molecules into cells. The amphiphiles contain lipophilic groups derived from steroids, from mono or dialkylamines, or from alkyl or acyl groups; and cationic groups, protonatable at physiological pH, derived from amines, alkylamines or polyalkylamines. There are provided also therapeutic compositions prepared typically by contacting a dispersion of one or more cationic amphiphiles with the therapeutic molecules. Therapeutic molecules that can be delivered into cells according to the practice of the invention include DNA, RNA, and polypeptides. Representative uses of the therapeutic compositions of the invention include providing gene therapy, and delivery of antisense polynucleotides or biologically active polypeptides to cells. With respect to therapeutic compositions for gene therapy, the DNA is provided typically in the form of a plasmid for complexing with the cationic amphiphile. Novel and highly effective plasmid constructs are also disclosed, including those that are particularly effective at providing gene therapy for clinical conditions complicated by inflammation. Additionally, targeting of organs for gene therapy by intravenous administration of therapeutic compositions is described.
摘要翻译： 提供了新的阳离子两亲物，其促进生物活性（治疗性）分子进入细胞。 两亲物含有衍生自类固醇的单亲或二烷基胺或烷基或酰基的亲脂基团; 和在生理pH下可质子化的阳离子基团，衍生自胺，烷基胺或多烷基胺。 还提供了通常通过使一种或多种阳离子两亲物的分散体与治疗分子接触而制备的治疗组合物。 根据本发明的实践可以递送到细胞中的治疗分子包括DNA，RNA和多肽。 本发明治疗组合物的代表性用途包括提供基因治疗以及向细胞递送反义多核苷酸或生物活性多肽。 关于基因治疗的治疗组合物，通常以与阳离子两亲物络合的质粒的形式提供DNA。 还公开了新型和高效的质粒构建体，包括在为炎症复杂的临床病症提供基因治疗方面特别有效的质粒构建体。 另外，描述了通过静脉内施用治疗组合物靶向用于基因治疗的器官。
-
公开(公告)号：US5877011A
公开(公告)日：1999-03-02
申请号：US752760
申请日：1996-11-20
IPC分类号： C12N15/09 , A61K48/00 , C07K14/075 , C12N15/861 , C12N15/86
CPC分类号： C12N15/86 , C07K14/005 , C12N2710/10322 , C12N2710/10343 , C12N2810/6018
摘要： A chimeric adenoviral vector is provided that comprises nucleotide sequence of a first adenovirus, wherein at least one gene of said first adenovirus encoding a protein that facilitates binding of said vector to a target mammalian cell, or internalization thereof within said cell, is replaced by the corresponding gene from a second adenovirus belonging to subgroup D, said vector further comprising a transgene operably linked to a eucaryotic promoter to allow for expression therefrom in a mammalian cell. Additionally, a method of delivering transgenes to target mammalian cells, particularly airway epithelial cells, is provided.
摘要翻译： 提供了包含第一腺病毒的核苷酸序列的嵌合腺病毒载体，其中编码促进所述载体与靶哺乳动物细胞结合的蛋白质的所述第一腺病毒的至少一个基因或其在所述细胞内的内化被替换为 来自属于亚组D的第二腺病毒的相应基因，所述载体还包含可操作地连接到真核启动子以在哺乳动物细胞中从其中表达的转基因。 另外，提供了将转基因递送至靶哺乳动物细胞，特别是气道上皮细胞的方法。
-
公开(公告)号：US5670488A
公开(公告)日：1997-09-23
申请号：US136742
申请日：1993-10-13
IPC分类号： A61K38/00 , A61K48/00 , C07K14/47 , C12N15/861 , C12N15/00
CPC分类号： C12N15/86 , A61K48/00 , C07K14/4712 , A61K38/00 , C12N2710/10343 , C12N2830/42 , C12N2840/203
摘要： Gene Therapy vectors, which are especially useful for cystic fibrosis, and methods for using the vectors are disclosed.
摘要翻译： 公开了对囊性纤维化特别有用的基因治疗载体和使用载体的方法。
-
公开(公告)号：US20080299083A1
公开(公告)日：2008-12-04
申请号：US11818907
申请日：2007-06-14
CPC分类号： C12N15/86 , A61K48/00 , C12N2710/10332 , C12N2710/10343 , C12N2830/008 , C12N2830/85
摘要： This invention provides a recombinant adenovirus expression vector characterized by the partial or total deletion of the adenoviral protein IX DNA and having a gene encoding a foreign protein or a functional fragment or mutant thereof. Transformed host cells and a method of producing recombinant proteins and gene therapy also are included within the scope of this invention.Thus, for example, the adenoviral vector of this invention can contain a foreign gene for the expression of a protein effective in regulating the cell cycle, such as p53, Rb, or mitosin, or in inducing cell death, such as the conditional suicide gene thymidine kinase. (The latter must be used in conjunction with a thymidine kinase metabolite in order to be effective).
-
-
-
-
-
-
-
-
-
搜索结果
27 条记录 (耗时 0.034 秒)
