公开(公告)号：US20180044640A1

公开(公告)日：2018-02-15

申请号：US15553285

申请日：2016-05-16

Applicant: Agency for Science, Technology and Research

Inventor： Hong Fang Lu ,  Meng Fatt Leong ,  Tze Chiun Lim ,  Andrew Chwee Aun WAN, Wan

IPC: C12N5/077 ,  G01N33/50 ,  G01N33/483

CPC classification number: C12N5/0657 ,  C12N5/0068 ,  C12N2500/36 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/15 ,  C12N2501/415 ,  C12N2501/999 ,  C12N2503/02 ,  C12N2503/04 ,  C12N2506/45 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2533/90 ,  C12N2535/00 ,  G01N33/4833 ,  G01N33/502 ,  G01N33/5061 ,  G01N33/5082

Abstract: The present invention relates to a method for producing a contractile cellular construct, comprising the steps of: a) seeding pre-selected cells onto a mold, wherein the pre-selected cells comprise signal emitting agents; and b) culturing the pre-selected cells to produce the cellular construct. The said method is exemplified by mixing cardiomyocytes with fluorescent microbeads, seeding the suspension on a polydimethylsiloxane (PDMS) mold and culturing to form a contractile device comprising of cardiac muscles. The present invention also relates to a method for measuring the contractility of the cellular construct by measuring the displacement of the signal emitting agents, and a method for screening a method for screening one or more agents for modulating the contractility of a contractile cellular construct.