公开(公告)号：US20210079389A1

公开(公告)日：2021-03-18

申请号：US17103678

申请日：2020-11-24

申请人： Agilent Technologies, Inc.

发明人： Daniel E. RYAN ,  Douglas J. DELLINGER ,  Jeffrey R. SAMPSON ,  Robert KAISER ,  Joel MYERSON

IPC分类号： C12N15/11 ,  C12N9/22 ,  C07H21/02 ,  C12N15/113 ,  C12N15/90 ,  C12Q1/6876

摘要： The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.

公开(公告)号：US20230122968A1

公开(公告)日：2023-04-20

申请号：US17986361

申请日：2022-11-14

申请人： Agilent Technologies, Inc.

发明人： Douglas J. DELLINGER ,  Joel MYERSON ,  Brian SMART

IPC分类号： C07D405/12 ,  C07D317/34 ,  C07D403/06 ,  C07H21/02

摘要： Compounds and methods for purifying oligonucleotides such as RNA and DNA. A target oligonucleotide is reacted with an orthoester linker comprising an affinity tag to form an orthoester oligonucleotide-orthoester linker conjugate which is subjected to a purification technique to separate the target oligonucleotide from impurities such as truncated oligonucleotides. The orthoester linker can he then removed under mild conditions to generate the target oligonucleotide in high purity.

公开(公告)号：US20200181124A1

公开(公告)日：2020-06-11

申请号：US16793808

申请日：2020-02-18

申请人： Agilent Technologies, Inc.

发明人： Douglas J. DELLINGER ,  Joel MYERSON ,  Brian SMART

IPC分类号： C07D405/12 ,  C07D403/06 ,  C07D317/34 ,  C07H21/02

摘要： Compounds and methods for purifying oligonucleotides such as RNA and DNA. A target oligonucleotide is reacted with an orthoester linker comprising an affinity tag to form an orthoester oligonucleotide-orthoester linker conjugate which is subjected to a purification technique to separate the target oligonucleotide from impurities such as truncated oligonucleotides. The orthoester linker can be then removed under mild conditions to generate the target oligonucleotide in high purity.