公开(公告)号：US20060223107A1

公开(公告)日：2006-10-05

申请号：US11447753

申请日：2006-06-05

申请人： Ahmed Chenna ,  Vivian Xiao ,  Sharat Singh

发明人： Ahmed Chenna ,  Vivian Xiao ,  Sharat Singh

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6816 ,  C07H19/06 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/682 ,  C12Q1/6823 ,  C40B20/08 ,  C40B40/08 ,  C40B50/16 ,  C40B70/00 ,  C12Q2565/125 ,  C12Q2537/162 ,  C12Q2521/301 ,  C12Q2537/125 ,  C12Q2525/161

摘要： The present invention is directed to a method of detecting pluralities of target nucleic acid sequences by forming and cleaving duplex structures with a pair of probes, one probe of each pair being labeled with an electrophoretic tag. Cleavage of the duplex structures releases electrophoretic tags that are then separated and identified to indicate the presence or quantity of the target sequences. The present invention is particularly useful in multiplex reactions wherein multiple target sequences are detected in one reaction. Kits useful in the detection of nucleic acids are also provided.

摘要翻译： 本发明涉及通过用一对探针形成和切割双链体结构来检测多个靶核酸序列的方法，每对中的一个探针用电泳标签标记。 双链结构的切割释放电泳标签，然后将其分离并鉴定以指示靶序列的存在或数量。 本发明特别适用于在一个反应​​中检测多个靶序列的多重反应。 还提供了用于检测核酸的试剂盒。

公开(公告)号：US06818399B2

公开(公告)日：2004-11-16

申请号：US09824984

申请日：2001-04-02

申请人： Sharat Singh ,  Hossein Salimi-Moosavi ,  Vivian Xiao

发明人： Sharat Singh ,  Hossein Salimi-Moosavi ,  Vivian Xiao

IPC分类号： C12O168

CPC分类号： C07H19/06 ,  C07H19/10 ,  C07H21/00 ,  C40B70/00 ,  G01N33/561 ,  Y10S435/81

摘要： Methods for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The methods include the use of electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. In practicing the methods, target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.

摘要翻译： 提供了多个检测一种或多种配体和靶向反配位体的结合或相互作用的方法。 检测涉及到识别标签作为目标识别的结果。 所述方法包括使用电泳标签探针或电子标签探针，其包含与靶结合部分连接的检测区域和称为迁移率调节剂的迁移率限定区域。 在实施该方法时，将目标抗配糖与一组e-标签探针接触，并用选定的切割剂处理所接触的抗配糖，导致电子标签报告子与未切割和/或部分切割的e-标签探针的混合物。 将混合物暴露于有效结合未切割或部分切割的e-标签探针的捕获剂，然后进行电泳分离。 在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。

公开(公告)号：US06627406B1

公开(公告)日：2003-09-30

申请号：US09568786

申请日：2000-05-10

申请人： Sharat Singh ,  Vivian Xiao ,  Ian Gibbons ,  Travis Boone ,  Torleif Ove Bjornson ,  Herbert H. Hooper ,  Edwin F. Ullman

发明人： Sharat Singh ,  Vivian Xiao ,  Ian Gibbons ,  Travis Boone ,  Torleif Ove Bjornson ,  Herbert H. Hooper ,  Edwin F. Ullman

IPC分类号： G01N3353

CPC分类号： B01L3/5027 ,  B01F13/0059 ,  B01J19/16 ,  B01J2219/00317 ,  B01L3/5025 ,  B01L3/502723 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2300/0861 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0688 ,  C40B60/14 ,  G01N1/34 ,  G01N2035/00237 ,  G01N2035/00287

摘要： Devices and methods are provided using microfluidic devices for manipulating small volumes and determining a variety of chemical and physical events. The devices rely upon an opening to the atmosphere of a small volume in a zone, where a sample is placed in the zone where evaporation can occur. The zone is maintained in contact with a liquid medium that serves to replenish the liquid in the zone and maintain the composition of the mixture in the zone substantially constant. The diffusion of components in the zone is restricted during the course of the determination by the liquid flux into the zone.