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    System of components for preparing oligonucleotides
    2.
    发明授权
    System of components for preparing oligonucleotides 失效
    制备寡核苷酸的组分体系

    公开(公告)号：US07321828B2

    公开(公告)日：2008-01-22

    申请号：US09067638

    申请日：1998-04-28

    申请人： Lex M. Cowsert ,  Brenda F. Baker ,  John McNeil ,  Susan M. Freier ,  Henri M. Sasmor ,  Douglas G. Brooks ,  Cara Ohashi ,  Jacqueline R. Wyatt ,  Alexander H. Borchers ,  Timothy A. Vickers

    发明人： Lex M. Cowsert ,  Brenda F. Baker ,  John McNeil ,  Susan M. Freier ,  Henri M. Sasmor ,  Douglas G. Brooks ,  Cara Ohashi ,  Jacqueline R. Wyatt ,  Alexander H. Borchers ,  Timothy A. Vickers

    IPC分类号： C12P19/34 ,  G06F19/00 ,  C12Q1/68

    CPC分类号： C12Q1/6811 ,  B01J2219/007 ,  C12N15/1048 ,  C40B50/02 ,  G06F19/18 ,  G06F19/22

    摘要： Interative, preferably computer based iterative processes for generating synthetic compounds with desired physical, chemical and/or bioactive properties, i.e., active compounds, are provided. During iterations of the processes, a target nucleic acid sequence is provided or selected, and a library of candidate nucleobase sequences is generated in silico according to defined criteria. A “virtual” oligonucleotide chemistry is chosen and a library of virtual oligonucleotide compounds having the selected nucleobase sequences is generated. These virtual compounds are reviewed and compounds predicted to have particular properties are selected. The selected compounds are robotically synthesized and are preferably robotically assayed for a desired physical, chemical or biological activity. Active compounds are thus generated and, at the same time, preferred sequences and regions of the target nucleic acid that are amenable to oligonucleotide or sequence-based modulation are identified.

    摘要翻译： 提供了用于产生具有期望的物理，化学和/或生物活性的合成化合物（即活性化合物）的相互优选的基于计算机的迭代方法。 在该过程的迭代过程中，提供或选择靶核酸序列，并且根据确定的标准以计算机生成候选核碱基序列的文库。 选择“虚拟”寡核苷酸化学，并产生具有选定的核碱基序列的虚拟寡核苷酸化合物文库。 审查这些虚拟化合物，并选择预测具有特定性质的化合物。 所选择的化合物是机械合成的，并且优选机械地测定所需的物理，化学或生物活性。 因此产生活性化合物，并且同时确定适用于寡核苷酸或基于序列的调节的靶核酸的优选序列和区域。

    USE OF ANTISENSE OLIGONUCLEOTIDE LIBRARIES FOR IDENTIFYING GENE FUNCTION
    3.
    发明申请
    USE OF ANTISENSE OLIGONUCLEOTIDE LIBRARIES FOR IDENTIFYING GENE FUNCTION 审中-公开
    使用反义寡核苷酸文库识别基因功能

    公开(公告)号：US20090023599A1

    公开(公告)日：2009-01-22

    申请号：US12179479

    申请日：2008-07-24

    申请人： C. Frank Bennett ,  Alexander H. Borchers ,  James G. Karras

    发明人： C. Frank Bennett ,  Alexander H. Borchers ,  James G. Karras

    IPC分类号： C40B30/06 ,  C40B40/06

    CPC分类号： C12N15/1136 ,  C12N15/1034 ,  Y02P20/582

    摘要： A method for identifying one or more genes involved in a phenotype of cells, tissues or organisms, comprising the steps of contacting cells, tissues or organisms which exhibit the phenotype with a library of antisense oligonucleotides and performing a primary phenotypic assay to determine which antisense oligonucleotides in the library attenuate the phenotype. These antisense oligonucleotides correspond to genes involved in the phenotype. The method may be used to identify genes involved in various disease states.

    摘要翻译： 用于鉴定涉及细胞，组织或生物体的表型的一种或多种基因的方法，包括以下步骤：将表现出表型的细胞，组织或生物与反义寡核苷酸文库接触，并进行初步表型测定以确定哪些反义寡核苷酸 在图书馆中减弱表型。 这些反义寡核苷酸对应于表型参与的基因。 该方法可用于鉴定参与各种疾病状态的基因。

    USE OF ANTISENSE OLIGONUCLEOTIDE LIBRARIES FOR IDENTIFYING GENE FUNCTION
    4.
    发明申请
    USE OF ANTISENSE OLIGONUCLEOTIDE LIBRARIES FOR IDENTIFYING GENE FUNCTION 审中-公开
    使用反义寡核苷酸文库识别基因功能

    公开(公告)号：US20100222231A1

    公开(公告)日：2010-09-02

    申请号：US12716900

    申请日：2010-03-03

    申请人： C. Frank Bennett ,  Alexander H. Borchers ,  James G. Karras

    发明人： C. Frank Bennett ,  Alexander H. Borchers ,  James G. Karras

    IPC分类号： C40B30/04 ,  C40B40/06

    CPC分类号： C12N15/1136 ,  C12N15/1034 ,  Y02P20/582

    摘要： A method for identifying one or more genes involved in a phenotype of cells, tissues or organisms, comprising the steps of contacting cells, tissues or organisms which exhibit the phenotype with a library of antisense oligonucleotides and performing a primary phenotypic assay to determine which antisense oligonucleotides in the library attenuate the phenotype. These antisense oligonucleotides correspond to genes involved in the phenotype. The method may be used to identify genes involved in various disease states.

    摘要翻译： 用于鉴定涉及细胞，组织或生物体的表型的一种或多种基因的方法，包括以下步骤：将表现出表型的细胞，组织或生物与反义寡核苷酸文库接触，并进行初步表型测定以确定哪些反义寡核苷酸 在图书馆中减弱表型。 这些反义寡核苷酸对应于表型参与的基因。 该方法可用于鉴定参与各种疾病状态的基因。