公开(公告)号：US08916363B2

公开(公告)日：2014-12-23

申请号：US12908454

申请日：2010-10-20

申请人： Alexander V. Gusakov ,  Tatyana N. Salanovich ,  Alexey I. Antonov ,  Boris B. Ustinov ,  Oleg N. Okunev ,  Richard P. Burlingame ,  Mark A. Emalfarb ,  Marco A. Baez ,  Arkady P. Sinitsyn

发明人： Alexander V. Gusakov ,  Tatyana N. Salanovich ,  Alexey I. Antonov ,  Boris B. Ustinov ,  Oleg N. Okunev ,  Richard P. Burlingame ,  Mark A. Emalfarb ,  Marco A. Baez ,  Arkady P. Sinitsyn

IPC分类号： C12P19/22 ,  C12P19/14 ,  C12N9/42

CPC分类号： C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16

摘要： This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

摘要翻译： 本发明提供使用新鉴定和分离的C.幸运感酶的新型酶组合物，其包括CBH Ib CBH IIb，EG II，EG VI，葡萄糖苷酶和木聚糖酶II与先前鉴定的酶CBH 1a，CBH IIa（先前描述） 作为Endo 43）和EG V.这些酶组合物显示出将木质纤维素生物质（例如，通过有机溶剂预处理的Avicel，棉花，道格拉斯冷杉木材）转化为葡萄糖的极高的能力。 两者具有纤维素结合模块（CBM）的CBH 1a和IIb显示出与来自棉花水解的相同真菌的三种主要内切葡聚糖酶（EG II，EG V，EG VI）的显着协同作用，以及与每种 其他。 酶组合物在木质纤维素生物质的水解中是有效的。

公开(公告)号：US08673618B2

公开(公告)日：2014-03-18

申请号：US12908483

申请日：2010-10-20

申请人： Alexander V. Gusakov ,  Tatyana N. Salanovich ,  Alexey I. Antonov ,  Boris B. Ustinov ,  Oleg N. Okunev ,  Richard P. Burlingame ,  Mark A. Emalfarb ,  Marco A. Baez ,  Arkady P. Sinitsyn

发明人： Alexander V. Gusakov ,  Tatyana N. Salanovich ,  Alexey I. Antonov ,  Boris B. Ustinov ,  Oleg N. Okunev ,  Richard P. Burlingame ,  Mark A. Emalfarb ,  Marco A. Baez ,  Arkady P. Sinitsyn

IPC分类号： C12N1/15

CPC分类号： C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16

摘要： This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

摘要翻译： 本发明提供使用新鉴定和分离的C.运气型酶的新型酶组合物，包括CBH Ib CBH IIb，EG II，EG VI，β-葡糖苷酶和木聚糖酶II结合先前鉴定的酶CBH 1a，CBH IIa（以前描述为 Endo 43）和EG V.这些酶组合物表现出将木质纤维素生物质（例如，通过有机溶剂预处理的Avicel，棉花，Douglas冷杉木材）转化为葡萄糖的极高的能力。 两者具有纤维素结合模块（CBM）的CBH 1a和IIb显示出与来自棉花水解的相同真菌的三种主要内切葡聚糖酶（EG II，EG V，EG VI）的显着协同作用，以及与每种 其他。 酶组合物在木质纤维素生物质的水解中是有效的。

公开(公告)号：US07883872B2

公开(公告)日：2011-02-08

申请号：US11487547

申请日：2006-07-13

申请人： Alexander V. Gusakov ,  Tatyana N. Salanovich ,  Alexey I. Antonov ,  Boris B. Ustinov ,  Oleg N. Okunev ,  Richard P. Burlingame ,  Mark A. Emalfarb ,  Marco A. Baez ,  Arkady P. Sinitsyn

发明人： Alexander V. Gusakov ,  Tatyana N. Salanovich ,  Alexey I. Antonov ,  Boris B. Ustinov ,  Oleg N. Okunev ,  Richard P. Burlingame ,  Mark A. Emalfarb ,  Marco A. Baez ,  Arkady P. Sinitsyn

IPC分类号： C12P19/16 ,  C12P19/20

CPC分类号： C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16

摘要： This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

摘要翻译： 本发明提供使用新鉴定和分离的C.幸运感酶的新型酶组合物，其包括CBH Ib CBH IIb，EG II，EG VI，葡萄糖苷酶和木聚糖酶II与先前鉴定的酶CBH 1a，CBH IIa（先前描述） 作为Endo 43）和EG V.这些酶组合物显示出将木质纤维素生物质（例如，通过有机溶剂预处理的Avicel，棉花，道格拉斯冷杉木材）转化为葡萄糖的极高的能力。 两者具有纤维素结合模块（CBM）的CBH 1a和IIb显示出与来自棉花水解的相同真菌的三种主要内切葡聚糖酶（EG II，EG V，EG VI）的显着协同作用，以及与每种 其他。 酶组合物在木质纤维素生物质的水解中是有效的。

公开(公告)号：US06764699B2

公开(公告)日：2004-07-20

申请号：US10167511

申请日：2002-06-13

申请人： Manuel J. Rubio ,  Roberto Contreras ,  Marco A. Baez

发明人： Manuel J. Rubio ,  Roberto Contreras ,  Marco A. Baez

IPC分类号： A23L1105

CPC分类号： A21D2/02 ,  A21D8/042 ,  A21D13/42 ,  A23L7/107 ,  A23L7/117 ,  C12Y302/01004 ,  C12Y302/01032

摘要： Corn masa dough texture and tortilla flexibility are improved by adding to the nixtamalized corn flour, an enzyme blend comprising commercial hemicellulase and/or cellulase. The enzyme preparation has a positive effect on dough texture and improvement in tortilla elasticity and ductility during commercial storage. The combination of the enzyme blend and the method for dough and tortilla preparation can enhance conventional additives to delay corn tortilla hardening during storage.

摘要翻译： 通过加入含有商业半纤维素酶和/或纤维素酶的酶混合物，加入玉米淀粉来提高玉米酱质地和玉米饼的柔韧性。 酶制剂对面团质地有正面影响，商品储存期间玉米粉弹性和延展性的改善。 酶混合物和面团和玉米粉制备方法的组合可以增强常规添加剂，以在储存期间延迟玉米玉米饼硬化。