公开(公告)号：US08609371B2

公开(公告)日：2013-12-17

申请号：US12540050

申请日：2009-08-12

申请人： Bryan Julien ,  Richard P. Burlingame

发明人： Bryan Julien ,  Richard P. Burlingame

IPC分类号： C12P21/00 ,  C12P19/34 ,  C12P5/00 ,  C07H21/04

CPC分类号： C12N9/1085 ,  C12N15/1079 ,  C12P5/002 ,  C12P5/007

摘要： The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

摘要翻译： 本发明涉及变体角鲨烯合酶，包括酿酒酵母角鲨烯合酶，以及编码这些变体酶的核酸分子。 这些变体酶以比野生型酶低的速率产生角鲨烯，允许更多的法呢基焦磷酸盐用于生产异戊二烯化合物，同时仍然产生足够的角鲨烯以允许酿酒酵母细胞生长而不需要由甾醇补充 如麦角甾醇。 因此，这些变体酶非常适合有效生产类异戊二烯。

公开(公告)号：US08673618B2

公开(公告)日：2014-03-18

申请号：US12908483

申请日：2010-10-20

申请人： Alexander V. Gusakov ,  Tatyana N. Salanovich ,  Alexey I. Antonov ,  Boris B. Ustinov ,  Oleg N. Okunev ,  Richard P. Burlingame ,  Mark A. Emalfarb ,  Marco A. Baez ,  Arkady P. Sinitsyn

发明人： Alexander V. Gusakov ,  Tatyana N. Salanovich ,  Alexey I. Antonov ,  Boris B. Ustinov ,  Oleg N. Okunev ,  Richard P. Burlingame ,  Mark A. Emalfarb ,  Marco A. Baez ,  Arkady P. Sinitsyn

IPC分类号： C12N1/15

CPC分类号： C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16

摘要： This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

摘要翻译： 本发明提供使用新鉴定和分离的C.运气型酶的新型酶组合物，包括CBH Ib CBH IIb，EG II，EG VI，β-葡糖苷酶和木聚糖酶II结合先前鉴定的酶CBH 1a，CBH IIa（以前描述为 Endo 43）和EG V.这些酶组合物表现出将木质纤维素生物质（例如，通过有机溶剂预处理的Avicel，棉花，Douglas冷杉木材）转化为葡萄糖的极高的能力。 两者具有纤维素结合模块（CBM）的CBH 1a和IIb显示出与来自棉花水解的相同真菌的三种主要内切葡聚糖酶（EG II，EG V，EG VI）的显着协同作用，以及与每种 其他。 酶组合物在木质纤维素生物质的水解中是有效的。

公开(公告)号：US07892812B2

公开(公告)日：2011-02-22

申请号：US09284152

申请日：1997-09-30

申请人： Mark Aaron Emalfarb ,  Irina Vladimirovna Solovjeva ,  Arie Ben-Bassat ,  Richard P. Burlingame ,  Vladimir Mikhaylovich Chernoglazov ,  Oleg Nicolaevich Ocounev ,  Philip T. Olson ,  Arkady Panteleimonovich Sinitsyn

发明人： Mark Aaron Emalfarb ,  Irina Vladimirovna Solovjeva ,  Arie Ben-Bassat ,  Richard P. Burlingame ,  Vladimir Mikhaylovich Chernoglazov ,  Oleg Nicolaevich Ocounev ,  Philip T. Olson ,  Arkady Panteleimonovich Sinitsyn

IPC分类号： C09B67/00 ,  C12N9/00 ,  C12N9/42 ,  C12N1/00 ,  D21C1/00

CPC分类号： D06P5/158 ,  C11D3/38645 ,  C11D11/0017 ,  C12N9/2437 ,  C12R1/645 ,  C12Y302/01004 ,  D06L4/40 ,  D06M16/003 ,  D06M2101/06 ,  D06P5/02

摘要： A computer-based method and apparatus for the analysis specification and support of work processes. The system is designed to support multiple interdependent decisions, at least some of which require collaboration among multiple participants (116). Work processes are modeled using an application framework (99) used to develop abstract, decision (100) process models. The decision (100) process models are used as a pattern to instantiate concrete process models that incorporate the work defined by the abstract process. The process model is then used to instantiate project models that incorporate the required work from the process. The project models are used to direct and guide the behavior of the participants (116) in the work process.

摘要翻译： 本发明涉及中和和/或碱性纤维素酶的新型组合物和从Chrysosporium培养物，特别是Chrysosporium lucknowense获得中性和/或碱性纤维素酶组合物的方法。 本发明还提供了产生能够产生中性和/或碱性纤维素酶的Chrysosporium突变体的突变体和方法。 本发明还涉及编码包含中性和/或碱性纤维素酶组合物的酶的基因。 此外，本发明提供培养Chrysosporium以产生中性和/或碱性纤维素酶的方法。 本发明的中性和/或碱性纤维素酶组合物可以用于各种工艺，包括洗衣服，洗涤剂工艺，脱墨，增色，脱色和纸和纸浆的生物漂白以及处理废物流。 本发明还涉及具有葡聚糖酶和纤维二糖水解酶活性的纤维素酶的分离和纯化，并且可用于石洗应用。

公开(公告)号：US07883872B2

公开(公告)日：2011-02-08

申请号：US11487547

申请日：2006-07-13

申请人： Alexander V. Gusakov ,  Tatyana N. Salanovich ,  Alexey I. Antonov ,  Boris B. Ustinov ,  Oleg N. Okunev ,  Richard P. Burlingame ,  Mark A. Emalfarb ,  Marco A. Baez ,  Arkady P. Sinitsyn

发明人： Alexander V. Gusakov ,  Tatyana N. Salanovich ,  Alexey I. Antonov ,  Boris B. Ustinov ,  Oleg N. Okunev ,  Richard P. Burlingame ,  Mark A. Emalfarb ,  Marco A. Baez ,  Arkady P. Sinitsyn

IPC分类号： C12P19/16 ,  C12P19/20

CPC分类号： C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16

摘要： This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

摘要翻译： 本发明提供使用新鉴定和分离的C.幸运感酶的新型酶组合物，其包括CBH Ib CBH IIb，EG II，EG VI，葡萄糖苷酶和木聚糖酶II与先前鉴定的酶CBH 1a，CBH IIa（先前描述） 作为Endo 43）和EG V.这些酶组合物显示出将木质纤维素生物质（例如，通过有机溶剂预处理的Avicel，棉花，道格拉斯冷杉木材）转化为葡萄糖的极高的能力。 两者具有纤维素结合模块（CBM）的CBH 1a和IIb显示出与来自棉花水解的相同真菌的三种主要内切葡聚糖酶（EG II，EG V，EG VI）的显着协同作用，以及与每种 其他。 酶组合物在木质纤维素生物质的水解中是有效的。

公开(公告)号：US06372457B1

公开(公告)日：2002-04-16

申请号：US09115475

申请日：1998-07-15

申请人： Alan Berry ,  Richard P. Burlingame ,  James R. Millis

发明人： Alan Berry ,  Richard P. Burlingame ,  James R. Millis

IPC分类号： C12P1900

CPC分类号： C07H5/06 ,  C07H11/04 ,  C12N9/1096 ,  C12N15/70 ,  C12P19/26 ,  C12Y206/01016

摘要： The present invention relates to a method and materials for producing glucosamine by fermentation of a genetically modified microorganism. Included in the present invention are genetically modified microorganisms useful in the present method for producing glucsamine, as well as recombinant nucleic acid molecules and the proteins produces by such recombinant nucleic acid molecules.

摘要翻译： 本发明涉及通过发酵转基因微生物生产葡糖胺的方法和材料。 本发明中包括用于制备葡糖胺的本发明方法中的遗传修饰的微生物，以及由这些重组核酸分子产生的重组核酸分子和蛋白质。

公开(公告)号：US5811381A

公开(公告)日：1998-09-22

申请号：US731170

申请日：1996-10-10

申请人： Mark Aaron Emalfarb ,  Arie Ben-Bassat ,  Richard P. Burlingame ,  Vladimir Mikhaylovich Chernoglazov ,  Oleg Nicolaevich Okounev ,  Philip T. Olson ,  Arkady Panteleimonovich Sinitsyn ,  Irina Vladimirovna Solovjeva

发明人： Mark Aaron Emalfarb ,  Arie Ben-Bassat ,  Richard P. Burlingame ,  Vladimir Mikhaylovich Chernoglazov ,  Oleg Nicolaevich Okounev ,  Philip T. Olson ,  Arkady Panteleimonovich Sinitsyn ,  Irina Vladimirovna Solovjeva

IPC分类号： C12N15/09 ,  C07K14/37 ,  C11D3/386 ,  C11D11/00 ,  C12N1/14 ,  C12N1/15 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N5/10 ,  C12N9/24 ,  C12N9/42 ,  C12N15/56 ,  C12Q1/68 ,  C12R1/645 ,  D06L3/11 ,  D06M16/00 ,  D06P5/02 ,  D06P5/15 ,  D21C9/10 ,  A61K38/47

CPC分类号： D06P5/158 ,  C11D11/0017 ,  C11D3/38645 ,  C12N9/2437 ,  C12R1/645 ,  C12Y302/01004 ,  D06L3/11 ,  D06M16/003 ,  D06P5/02 ,  D06M2101/06

摘要： The subject invention relates to novel compositions of neutral and/or alkaline cellulase and methods for obtaining neutral and/or alkaline cellulase compositions from Chrysosporium cultures, in particular Chrysosporium lucknowense. This invention also provides mutants and methods of generating mutants of Chrysosporium capable of producing neutral and/or alkaline cellulose. This invention also relates to the genes encoding the enzymes comprising the neutral and/or alkaline cellulase composition. In addition, this invention provides methods of culturing Chrysosporium to produce neutral and/or alkaline cellulases. The neutral and/or alkaline cellulase compositions of the subject invention can be used in a variety of processes including stone washing of clothing, detergent processes, deinking and biobleaching of paper & pulp and treatment of waste streams.

摘要翻译： 本发明涉及中和和/或碱性纤维素酶的新型组合物和从Chrysosporium培养物，特别是Chrysosporium lucknowense获得中性和/或碱性纤维素酶组合物的方法。 本发明还提供了能产生中性和/或碱性纤维素的Chrysosporium突变体的突变体和方法。 本发明还涉及编码包含中性和/或碱性纤维素酶组合物的酶的基因。 此外，本发明提供培养Chrysosporium以产生中性和/或碱性纤维素酶的方法。 本发明的中性和/或碱性纤维素酶组合物可以用于各种工艺，包括洗衣服，洗涤剂工艺，纸和纸浆的脱墨和生物漂白以及废物流的处理。

公开(公告)号：US08916363B2

公开(公告)日：2014-12-23

申请号：US12908454

申请日：2010-10-20

申请人： Alexander V. Gusakov ,  Tatyana N. Salanovich ,  Alexey I. Antonov ,  Boris B. Ustinov ,  Oleg N. Okunev ,  Richard P. Burlingame ,  Mark A. Emalfarb ,  Marco A. Baez ,  Arkady P. Sinitsyn

发明人： Alexander V. Gusakov ,  Tatyana N. Salanovich ,  Alexey I. Antonov ,  Boris B. Ustinov ,  Oleg N. Okunev ,  Richard P. Burlingame ,  Mark A. Emalfarb ,  Marco A. Baez ,  Arkady P. Sinitsyn

IPC分类号： C12P19/22 ,  C12P19/14 ,  C12N9/42

CPC分类号： C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16

摘要： This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

摘要翻译： 本发明提供使用新鉴定和分离的C.幸运感酶的新型酶组合物，其包括CBH Ib CBH IIb，EG II，EG VI，葡萄糖苷酶和木聚糖酶II与先前鉴定的酶CBH 1a，CBH IIa（先前描述） 作为Endo 43）和EG V.这些酶组合物显示出将木质纤维素生物质（例如，通过有机溶剂预处理的Avicel，棉花，道格拉斯冷杉木材）转化为葡萄糖的极高的能力。 两者具有纤维素结合模块（CBM）的CBH 1a和IIb显示出与来自棉花水解的相同真菌的三种主要内切葡聚糖酶（EG II，EG V，EG VI）的显着协同作用，以及与每种 其他。 酶组合物在木质纤维素生物质的水解中是有效的。

公开(公告)号：US20120246767A1

公开(公告)日：2012-09-27

申请号：US13317839

申请日：2011-10-28

申请人： Jean Davin Amick ,  Eunyoung Park ,  Bryan N. Julien ,  Richard P. Burlingame

发明人： Jean Davin Amick ,  Eunyoung Park ,  Bryan N. Julien ,  Richard P. Burlingame

IPC分类号： C12N9/00 ,  C12N15/63 ,  C12N1/21 ,  C12P7/26 ,  C12N5/10 ,  A01H5/00 ,  C12P5/00 ,  C12N15/52 ,  C12N1/19

CPC分类号： C12N9/88 ,  C07H21/04 ,  C07K14/415 ,  C12N15/81 ,  C12N15/82 ,  C12N15/8243 ,  C12N15/86

摘要： Provided are modified valencene synthase polypeptides and methods of using the modified valencene synthase polypeptides. Also provided are methods for producing modified terpene synthases.

摘要翻译： 提供了修饰的戊烯合酶多肽和使用修饰的戊烯合酶多肽的方法。 还提供了用于生产改性萜烯合酶的方法。

公开(公告)号：US5856148A

公开(公告)日：1999-01-05

申请号：US797727

申请日：1997-02-11

申请人： Richard P. Burlingame

发明人： Richard P. Burlingame

IPC分类号： C12N9/04 ,  C12N15/53

CPC分类号： C12N9/0006

摘要： Engineered DNA encoding 3-phosphoglycerate dehydrogenase (PGD) has reduced sensitivity to inhibition by serine in comparison to wild-type PGD. The DNA encodes PGD which has at least some level of enzymatic activity useful for biosynthesis, and which retains that activity at higher serine levels than does the (unmodified) wild-type PGD. The PGD has the amino acid sequence of this above-described engineered DNA. Expression vectors contain this engineered DNA and regulatory DNA positioned and oriented for expression of the engineered DNA in a host expression system. Cells contain these expression vectors, and a method for producing serine or a serine-derived product includes culturing such cells.

摘要翻译： 与野生型PGD相比，编码3-磷酸甘油酸脱氢酶（PGD）的工程DNA降低了对丝氨酸抑制的敏感性。 DNA编码PGD，其具有至少一些可用于生物合成的酶活性水平，并且在野生型（未修饰的）野生型PGD中保留比丝氨酸水平更高的活性。 PGD​​具有上述工程DNA的氨基酸序列。 表达载体含有这种工程化的DNA和调节DNA，其定位并定向于在宿主表达系统中表达工程化的DNA。 细胞含有这些表达载体，制备丝氨酸或丝氨酸衍生产物的方法包括培养这些细胞。

公开(公告)号：US5618716A

公开(公告)日：1997-04-08

申请号：US244491

申请日：1994-05-24

申请人： Richard P. Burlingame

发明人： Richard P. Burlingame

IPC分类号： C12N1/19 ,  C12N1/21 ,  C12N9/04 ,  C12N15/09 ,  C12N15/53 ,  C12P13/06 ,  C12P13/22 ,  C12R1/19 ,  C12R1/865

CPC分类号： C12N9/0006 ,  C12P13/06

摘要： Engineered DNA encoding 3-phosphoglycerate dehydrogenate (PGD) has reduced sensitivity to inhibition by serine in comparison to wild-type PGD. The DNA encodes PGD which has at least some level of enzymatic activity useful for biosynthesis, and which retains that activity at higher serine levels than does the (unmodified) wild-type PGD. The PGD has the amino acid sequence of this above-described engineered DNA. Expression vectors contain this engineered DNA and regulatory DNA positioned and oriented for expression of the engineered DNA in a host expression system. Cells contain these expression vectors, and a method for producing serine or a serine-derived product includes culturing such cells.

摘要翻译： PCT No.PCT / EP92 / 01873 Sec。 371日期：1994年5月24日 102（e）日期1994年5月24日PCT提交1992年8月17日PCT公布。 出版物WO93 / 12235 日期1993年6月24日编号3-磷酸甘油酸脱氢酶（PGD）的工程DNA比野生型PGD降低了对丝氨酸抑制的敏感性。 DNA编码PGD，其具有至少一些可用于生物合成的酶活性水平，并且在野生型（未修饰的）野生型PGD中保留比丝氨酸水平更高的活性。 PGD​​具有上述工程DNA的氨基酸序列。 表达载体含有这种工程化的DNA和调节DNA，其定位并定向于在宿主表达系统中表达工程化的DNA。 细胞含有这些表达载体，制备丝氨酸或丝氨酸衍生产物的方法包括培养这些细胞。