公开(公告)号：US20060240462A1

公开(公告)日：2006-10-26

申请号：US11409369

申请日：2006-04-21

申请人： Alison Todd ,  Tanya Applegate ,  Tram Doan ,  Paul Young

发明人： Alison Todd ,  Tanya Applegate ,  Tram Doan ,  Paul Young

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6816 ,  C12Q2531/119

摘要： Provided herein are methods for the combined amplification and detection of one or a plurality of target nucleic acid molecules. The methods encompass the use of an antisense strand of a catalytic nucleic acid in a primer for amplification such that an amplicon produced thereby includes an active catalytic nucleic acid capable of indicating the presence of the target sequences through the modification of a reporter substrate. Devices and kits are also provided. DNA molecules for practicing the methods are also provided. The DNA molecules comprise at least a first portion complementary to at least a first portion of a target nucleic acid sequence, a second portion complementary to an antisense sequence of a second portion of the target nucleic acid sequence, and a third portion comprising an antisense sequence of a catalytic nucleic acid; the third portion positioned between the first and second portions of said DNA molecule.

摘要翻译： 本文提供了用于组合扩增和检测一个或多个靶核酸分子的方法。 所述方法包括在引物中使用催化核酸的反义链进行扩增，使得由此产生的扩增子包含能够通过报道底物修饰来指示靶序列存在的活性催化核酸。 还提供设备和套件。 还提供了用于实践该方法的DNA分子。 DNA分子包含至少与靶核酸序列的至少第一部分互补的第一部分，与靶核酸序列的第二部分的反义序列互补的第二部分和包含反义序列的第三部分 的催化核酸; 位于所述DNA分子的第一和第二部分之间的第三部分。

公开(公告)号：US20070231810A1

公开(公告)日：2007-10-04

申请号：US11544926

申请日：2006-10-06

申请人： Alison Todd ,  Donald Birkett ,  Tram Doan ,  Elisa Mokany

发明人： Alison Todd ,  Donald Birkett ,  Tram Doan ,  Elisa Mokany

IPC分类号： C12Q1/68 ,  C07K14/00

CPC分类号： C07H21/04 ,  C12N15/111 ,  C12N2310/12 ,  C12N2320/10 ,  C12N2330/30 ,  C12Q1/6811 ,  C12Q1/6818 ,  C12Q1/682 ,  C12Q1/6823 ,  C12Q2537/125 ,  C12Q2525/161 ,  C12Q2521/337 ,  C12Q2525/205

摘要： The present invention relates to Multicomponent Nucleic Acid Enzymes (MNAzymes) and methods for their use. MNAzymes comprise two or more oligonucleotide components which self-assemble in the presence of one or more MNAzyme assembly facilitator molecules to form a catalytically active structure. Compositions for making MNAzymes, and collections of MNAzymes are provided. Also provided are methods for using MNAzymes for the detection, identification and/or quantification of one or more targets. The methods can be practiced in solution-based assays or in assays where one or more reaction components are attached to a support structure. The methods allow for multiplexing the MNAzyme detection to detect multiple targets in a single reaction. Also provided are kits for making the compositions, and for practicing the methods provided herein.

摘要翻译： 本发明涉及多组分核酸酶（MNAzymes）及其使用方法。 MNAzymes包含两种或更多种在一种或多种MNAzyme组装促进剂分子存在下自组装以形成催化活性结构的寡核苷酸组分。 提供制作MNAzymes的组合物，以及MNAzymes的集合。 还提供了使用MNAzymes检测，鉴定和/或定量一个或多个靶标的方法。 该方法可以在基于溶液的测定或在其中一个或多个反应组分连接到支持结构的测定中实施。 该方法允许复用MNAzyme检测以在单个反应中检测多个靶标。 还提供了用于制备组合物和用于实施本文提供的方法的试剂盒。

公开(公告)号：US20060210990A1

公开(公告)日：2006-09-21

申请号：US10563195

申请日：2004-07-05

申请人： Alison Todd ,  Caroline Fuery ,  Tanya Applegate

发明人： Alison Todd ,  Caroline Fuery ,  Tanya Applegate

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  C12Q2523/113 ,  C12Q2521/539 ,  C12Q2537/164

摘要： There is described a method for detecting alkylated cytosine in double stranded DNA employing one or more enzymes that differentially modify alkylated cytosine and cytosine. At least one region of the DNA is converted to single stranded DNA and the enzyme is reacted with a target region in the single stranded DNA. The presence or level of alkylated cytosine in the target region is detected by determining the level of enzymatic modification of the target region by the enzyme.

摘要翻译： 描述了使用差异修饰烷基化胞嘧啶和胞嘧啶的一种或多种酶检测双链DNA中烷基化胞嘧啶的方法。 将DNA的至少一个区域转化为单链DNA，并且酶与单链DNA中的靶区域反应。 通过酶测定靶区域的酶修饰水平来检测目标区域中烷基化胞嘧啶的存在或水平。