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    MULTICOMPONENT NUCLEIC ACID ENZYMES WITH CLEAVAGE, LIGASE OR OTHER ACTIVITY AND METHODS FOR THEIR USE
    7.
    发明申请
    MULTICOMPONENT NUCLEIC ACID ENZYMES WITH CLEAVAGE, LIGASE OR OTHER ACTIVITY AND METHODS FOR THEIR USE 审中-公开
    具有CLEAVAGE，LIGASE或其他活性的多功能核酸酶及其使用方法

    公开(公告)号：US20120101267A1

    公开(公告)日：2012-04-26

    申请号：US11697021

    申请日：2007-04-05

    申请人： Alison Velyian Todd ,  Elisa Mokany ,  Donald John Birkett ,  Tram Bich Doan ,  Paul Ean Young

    发明人： Alison Velyian Todd ,  Elisa Mokany ,  Donald John Birkett ,  Tram Bich Doan ,  Paul Ean Young

    IPC分类号： C07H21/00 ,  C09K3/00 ,  G01N33/50 ,  C07H21/04 ,  C12Q1/68

    CPC分类号： C12Q1/6811 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q2525/205 ,  C12Q2525/161 ,  C12Q2521/337

    摘要： The present invention relates to Multicomponent Nucleic Acid Enzymes (MNAzymes) and methods for their use. MNAzymes comprise two or more oligonucleotide components which self-assemble in the presence of one or more MNAzyme assembly facilitator molecules to form a catalytically active structure which may have cleavage, ligase or other enzymatic activity. Compositions for making MNAzymes and collections of MNAzymes are provided, together with uses thereof. Also provided are methods for using MNAzymes for the detection, identification and/or quantification of one or more targets. The methods can be practiced in solution-based assays or in assays where one or more reaction components are attached to a support structure. The methods allow for multiplexing the MNAzyme detection to detect multiple targets in a single reaction. Also provided are kits for making the compositions, and for practicing the methods provided herein.

    摘要翻译： 本发明涉及多组分核酸酶（MNAzymes）及其使用方法。 MNAzymes包含两种或更多种在一种或多种MNAzyme组装促进剂分子存在下自组装以形成可能具有切割，连接酶或其它酶活性的催化活性结构的寡核苷酸组分。 提供用于制备MNAzymes和MNAzymes的组合的组合及其用途。 还提供了使用MNAzymes检测，鉴定和/或定量一个或多个靶标的方法。 该方法可以在基于溶液的测定或在其中一个或多个反应组分连接到支持结构的测定中实施。 该方法允许复用MNAzyme检测以在单个反应中检测多个靶标。 还提供了用于制备组合物和用于实施本文提供的方法的试剂盒。

    Multicomponent nucleic acid enzymes and methods for their use
    10.
    发明申请
    Multicomponent nucleic acid enzymes and methods for their use 有权
    多组分核酸酶及其使用方法

    公开(公告)号：US20070231810A1

    公开(公告)日：2007-10-04

    申请号：US11544926

    申请日：2006-10-06

    申请人： Alison Todd ,  Donald Birkett ,  Tram Doan ,  Elisa Mokany

    发明人： Alison Todd ,  Donald Birkett ,  Tram Doan ,  Elisa Mokany

    IPC分类号： C12Q1/68 ,  C07K14/00

    CPC分类号： C07H21/04 ,  C12N15/111 ,  C12N2310/12 ,  C12N2320/10 ,  C12N2330/30 ,  C12Q1/6811 ,  C12Q1/6818 ,  C12Q1/682 ,  C12Q1/6823 ,  C12Q2537/125 ,  C12Q2525/161 ,  C12Q2521/337 ,  C12Q2525/205

    摘要： The present invention relates to Multicomponent Nucleic Acid Enzymes (MNAzymes) and methods for their use. MNAzymes comprise two or more oligonucleotide components which self-assemble in the presence of one or more MNAzyme assembly facilitator molecules to form a catalytically active structure. Compositions for making MNAzymes, and collections of MNAzymes are provided. Also provided are methods for using MNAzymes for the detection, identification and/or quantification of one or more targets. The methods can be practiced in solution-based assays or in assays where one or more reaction components are attached to a support structure. The methods allow for multiplexing the MNAzyme detection to detect multiple targets in a single reaction. Also provided are kits for making the compositions, and for practicing the methods provided herein.

    摘要翻译： 本发明涉及多组分核酸酶（MNAzymes）及其使用方法。 MNAzymes包含两种或更多种在一种或多种MNAzyme组装促进剂分子存在下自组装以形成催化活性结构的寡核苷酸组分。 提供制作MNAzymes的组合物，以及MNAzymes的集合。 还提供了使用MNAzymes检测，鉴定和/或定量一个或多个靶标的方法。 该方法可以在基于溶液的测定或在其中一个或多个反应组分连接到支持结构的测定中实施。 该方法允许复用MNAzyme检测以在单个反应中检测多个靶标。 还提供了用于制备组合物和用于实施本文提供的方法的试剂盒。