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公开(公告)号：US08058410B2
公开(公告)日：2011-11-15
申请号：US11919258
申请日：2006-04-25
申请人： Alois Jungbauer , Rainer Hahn , Waltraud Kaar , Michael Seifert , Bernhard Auer , Clemens Achmüller , Philipp Wechner
发明人： Alois Jungbauer , Rainer Hahn , Waltraud Kaar , Michael Seifert , Bernhard Auer , Clemens Achmüller , Philipp Wechner
摘要： Disclosed is an affinity matrix comprising a solid phase and an affinity ligand comprising peptide bonds coupled to this solid phase, wherein the affinity ligand comprising peptide bond is selected from the following group of ligands: a) peptides comprising the formula X1X2X3X4, wherein X1 to X4 are amino acid residues and at least two of X1 to X4 is W, Y or F; b) peptides comprising the formula X5X6X7X8, wherein X5 to X8 are amino acid residues, at least one of X5 to X8 is W, and at least one of X5 to X8 is E or D; and c) poly-amino acids consisting of an amino acid monomer of the group consisting of R, K, E and D and an amino acid monomer of the group consisting of Y, F and W, preferably poly-KY, poly-KF, poly-KW, poly-RY, poly-RF, poly-RW, poly-EY, poly-DY, poly-EF, poly-EW, poly-DF and poly-DW, with the proviso that the peptides according to a) and b) have a maximum length of 35 amino acid residues and that the poly-amino acids according to c) have a minimum length of 20 amino acid residues.
摘要翻译： 公开了包含固相和包含与该固相偶联的肽键的亲和配体的亲和基质，其中包含肽键的亲和配体选自以下配体组：a）包含式X1X2X3X4的肽，其中X1至X4 是氨基酸残基，X1至X4中的至少两个为W，Y或F; b）包含式X5X6X7X8的肽，其中X5至X8为氨基酸残基，X5至X8中的至少一个为W，X5至X8中的至少一个为E或D; 和c）由R，K，E和D组成的组的氨基酸单体和由Y，F和W组成的组的氨基酸单体组成的聚氨基酸，优选聚KY，聚-KF， poly-KW，poly-RY，poly-RF，poly-RW，poly-EY，poly-DY，poly-EF，poly-EW，poly-DF和poly-DW，条件是根据a） 和b）具有35个氨基酸残基的最大长度，并且根据c）的多氨基酸具有20个氨基酸残基的最小长度。
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公开(公告)号：US20090306343A1
公开(公告)日：2009-12-10
申请号：US11919258
申请日：2006-04-25
申请人： Alois Jungbauer , Rainer Hahn , Waltraud Kaar , Michael Seifert , Bernhard Auer , Clemens Achmuller , Philipp Wechner
发明人： Alois Jungbauer , Rainer Hahn , Waltraud Kaar , Michael Seifert , Bernhard Auer , Clemens Achmuller , Philipp Wechner
摘要： Disclosed is an affinity matrix comprising a solid phase and an affinity ligand comprising peptide bonds coupled to this solid phase, wherein the affinity ligand comprising peptide bond is selected from the following group of ligands: a) peptides comprising the formula X1X2X3X4, wherein X1 to X4 are amino acid residues and at least two of X1 to X4 is W, Y or F; b) peptides comprising the formula X5X6X7X8, wherein X5 to X8 are amino acid residues, at least one of X5 to X8 is W, and at least one of X5 to X8 is E or D; and c) poly-amino acids consisting of an amino acid monomer of the group consisting of R, K, E and D and an amino acid monomer of the group consisting of Y, F and W, preferably poly-KY, poly-KF, poly-KW, poly-RY, poly-RF, poly-RW, poly-EY, poly-DY, poly-EF, poly-EW, poly-DF and poly-DW, with the proviso that the peptides according to a) and b) have a maximum length of 35 amino acid residues and that the poly-amino acids according to c) have a minimum length of 20 amino acid residues.
摘要翻译： 公开了包含固相和包含与该固相偶联的肽键的亲和配体的亲和基质，其中包含肽键的亲和配体选自以下配体组：a）包含式X1X2X3X4的肽，其中X1至X4 是氨基酸残基，X1至X4中的至少两个为W，Y或F; b）包含式X5X6X7X8的肽，其中X5至X8为氨基酸残基，X5至X8中的至少一个为W，X5至X8中的至少一个为E或D; 和c）由R，K，E和D组成的组的氨基酸单体和由Y，F和W组成的组的氨基酸单体组成的聚氨基酸，优选聚KY，聚-KF， poly-KW，poly-RY，poly-RF，poly-RW，poly-EY，poly-DY，poly-EF，poly-EW，poly-DF和poly-DW，条件是根据a） 和b）具有35个氨基酸残基的最大长度，并且根据c）的多氨基酸具有20个氨基酸残基的最小长度。
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3.发明授权Production of recombinant proteins by autoproteolytic cleavage of a fusion protein 有权通过自身蛋白水解切割融合蛋白来生产重组蛋白公开(公告)号：US08372959B2
公开(公告)日：2013-02-12
申请号：US13348607
申请日：2012-01-11
摘要： Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprises the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein the steps are conducted on one affinity chromatography system.
摘要翻译： 公开了使用包含感兴趣的多肽的融合多肽和N末端具有表达自身蛋白水解功能的多肽来产生具有均质N末端的异源多肽的方法，所述方法包括以下步骤：a） 通过亲和层析系统以可溶性，自动蛋白水解无活性形式的融合多肽，b）融合多肽的折叠，从而激活融合多肽的自体蛋白水解功能，并导致感兴趣的异源多肽切割，以及c）随后将异源 多肽，其中所述步骤在一个亲和层析系统上进行。
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4.发明授权Production of recombinant proteins by autoproteolytic cleavage of a fusion protein 有权通过自身蛋白水解切割融合蛋白来生产重组蛋白公开(公告)号：US08163890B2
公开(公告)日：2012-04-24
申请号：US11919257
申请日：2006-04-25
摘要： Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprising the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein said steps are conducted on one affinity chromatography system.
摘要翻译： 公开了使用包含感兴趣的多肽的融合多肽和N末端具有表达自身蛋白水解功能的多肽来产生具有均质N末端的异源多肽的方法，所述方法包括以下步骤：a） 通过亲和层析系统以可溶性，自动蛋白水解无活性形式的融合多肽，b）融合多肽的折叠，从而激活融合多肽的自体蛋白水解功能，并导致感兴趣的异源多肽切割，以及c）随后将异源 多肽，其中所述步骤在一个亲和层析系统上进行。
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公开(公告)号：US20090203069A1
公开(公告)日：2009-08-13
申请号：US11919257
申请日：2006-04-25
摘要： Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprising the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein said steps are conducted on one affinity chromatography system.
摘要翻译： 公开了使用包含感兴趣的多肽的融合多肽和N末端具有表达自身蛋白水解功能的多肽来产生具有均质N末端的异源多肽的方法，所述方法包括以下步骤：a） 通过亲和层析系统以可溶性，自动蛋白水解无活性形式的融合多肽，b）融合多肽的折叠，从而激活融合多肽的自体蛋白水解功能，并导致感兴趣的异源多肽切割，以及c）随后将异源 多肽，其中所述步骤在一个亲和层析系统上进行。
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公开(公告)号：US09901597B2
公开(公告)日：2018-02-27
申请号：US13980894
申请日：2012-02-03
申请人： Waltraud Kaar , Alfred Zochling , Karin Ahrer
发明人： Waltraud Kaar , Alfred Zochling , Karin Ahrer
摘要： A method for inactivation or removal of coagulation factors FII, FVII, FVIIa, FIX, FIXa, FX, FXI and FXIa in or from protein containing solutions obtained from blood, blood plasma, plasma fractions or by recombinant means wherein the protein containing solution is contacted with an organic acid or its salt while being stirred.
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公开(公告)号：US20140007547A1
公开(公告)日：2014-01-09
申请号：US13980894
申请日：2012-02-03
申请人： Waltraud Kaar , Alfred Zochling , Karin Ahrer
发明人： Waltraud Kaar , Alfred Zochling , Karin Ahrer
摘要： A method for inactivation or removal of coagulation factors FII, FVII, FVIIa, FIX, FIXa, FX, FXI and FXIa in or from protein containing solutions obtained from blood, blood plasma, plasma fractions or by recombinant means wherein the protein containing solution is contacted with an organic acid or its salt while being stirred.
摘要翻译： 在血液，血浆，血浆级分中获得的含蛋白质溶液中或通过重组方法灭活或除去凝血因子FII，FVII，FVIIa，FIX，FIXa，FX，FXI和FXIa的方法，其中所述含蛋白质溶液被接触 与有机酸或其盐同时搅拌。
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公开(公告)号：US20100273983A1
公开(公告)日：2010-10-28
申请号：US12594821
申请日：2008-04-04
摘要： A method is provided for purifying peptides by selective precipitation of contaminating proteins, such as host cell proteins and cleaved fusion partners. Also provided is a method of cleaving fusion proteins in cell lysates.
摘要翻译： 提供了通过选择性沉淀污染性蛋白质（例如宿主细胞蛋白质和切割的融合配偶体）来纯化肽的方法。 还提供了在细胞裂解物中切割融合蛋白的方法。
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