公开(公告)号：US08871069B2

公开(公告)日：2014-10-28

申请号：US13154088

申请日：2011-06-06

申请人： Amy H. Chu ,  Huan-Ping Wu ,  Boru Zhu

发明人： Amy H. Chu ,  Huan-Ping Wu ,  Boru Zhu

IPC分类号： G01N27/327 ,  G01N33/543 ,  C12Q1/00

CPC分类号： C12Q1/004 ,  G01N33/5438

摘要： A biosensor system for determining the concentration of an analyte in a sample is disclosed that includes a reaction means for selectively performing a redox reaction of an analyte, and a measurement means for measuring a rate of the redox reaction of the analyte. The reaction means includes a binder, a buffer salt, a mediator including at most 20% (w/w) of an inorganic, non-transition metal salt, and an enzyme system. The measurement means includes at least two conductors. The measurement means measures an output signal value from the reaction means at a maximum kinetic performance within at most 7 seconds of introducing a sample to the reaction means, where the output signal value is responsive to the concentration of the analyte in the sample, and the measurement means determines at least one ΔS value responsive to at least one error parameter. The measurement means further determines the analyte concentration in the sample from a compensation equation including at least one reference correlation and the at least one ΔS value, where the compensation equation has a R2 value of at least 0.5.

摘要翻译： 公开了一种用于确定样品中分析物浓度的生物传感器系统，其包括用于选择性地进行分析物的氧化还原反应的反应装置和用于测量分析物的氧化还原反应速率的测量装置。 反应装置包括粘合剂，缓冲盐，包含至多20％（w / w）的无机非过渡金属盐和酶体系的介体。 测量装置包括至少两个导体。 测量装置在将样品引入反应装置的最多动力学性能的最大动力学性能下测量来自反应装置的输出信号值，其中输出信号值响应样品中分析物的浓度， 测量装置根据至少一个误差参数确定至少一个＆Dgr S值。 测量装置进一步从包括至少一个参考相关性和至少一个＆Dgr S的值的补偿方程确定样品中的分析物浓度，其中补偿方程的R2值至少为0.5。

公开(公告)号：US20110297540A1

公开(公告)日：2011-12-08

申请号：US13154088

申请日：2011-06-06

申请人： Amy H. Chu ,  Huan-Ping Wu ,  Boru Zhu

发明人： Amy H. Chu ,  Huan-Ping Wu ,  Boru Zhu

IPC分类号： G01N27/327 ,  G01N33/50

CPC分类号： C12Q1/004 ,  G01N33/5438

摘要： A biosensor system for determining the concentration of an analyte in a sample is disclosed that includes a reaction means for selectively performing a redox reaction of an analyte, and a measurement means for measuring a rate of the redox reaction of the analyte. The reaction means includes a binder, a buffer salt, a mediator including at most 20% (w/w) of an inorganic, non-transition metal salt, and an enzyme system. The measurement means includes at least two conductors. The measurement means measures an output signal value from the reaction means at a maximum kinetic performance within at most 7 seconds of introducing a sample to the reaction means, where the output signal value is responsive to the concentration of the analyte in the sample, and the measurement means determines at least one ΔS value responsive to at least one error parameter. The measurement means further determines the analyte concentration in the sample from a compensation equation including at least one reference correlation and the at least one ΔS value, where the compensation equation has a R2 value of at least 0.5.

摘要翻译： 公开了一种用于确定样品中分析物浓度的生物传感器系统，其包括用于选择性地进行分析物的氧化还原反应的反应装置和用于测量分析物的氧化还原反应速率的测量装置。 反应装置包括粘合剂，缓冲盐，包含至多20％（w / w）的无机非过渡金属盐和酶体系的介体。 测量装置包括至少两个导体。 测量装置在将样品引入反应装置的最多动力学性能的最大动力学性能下测量来自反应装置的输出信号值，其中输出信号值响应样品中分析物的浓度， 测量装置根据至少一个误差参数确定至少一个＆Dgr S值。 测量装置进一步从包括至少一个参考相关性和至少一个＆Dgr S的值的补偿方程确定样品中的分析物浓度，其中补偿方程的R2值至少为0.5。

公开(公告)号：US08470604B2

公开(公告)日：2013-06-25

申请号：US13210738

申请日：2011-08-16

申请人： Huan-Ping Wu ,  Steven C. Charlton ,  Amy H. Chu ,  Andrew J. Edelbrock ,  Sung-Kwon Jung ,  Dijia Huang

发明人： Huan-Ping Wu ,  Steven C. Charlton ,  Amy H. Chu ,  Andrew J. Edelbrock ,  Sung-Kwon Jung ,  Dijia Huang

IPC分类号： G01N27/333 ,  G01N33/66

CPC分类号： C12Q1/54 ,  A61B5/14532 ,  A61B5/1455 ,  A61B5/1486 ,  A61B5/1495 ,  C12Q1/004 ,  G01N27/3271 ,  G01N27/3272

摘要： A biosensor system determines an analyte concentration of a biological sample using an electrochemical process without Cottrell decay. The biosensor system generates an output signal having a transient decay, where the output signal is not inversely proportional to the square root of the time. The transient decay is greater or less than the −0.5 decay constant of a Cottrell decay. The transient decay may result from a relatively short incubation period, relatively small sample reservoir volumes, relatively small distances between electrode surfaces and the lid of the sensor strip, and/or relatively short excitations in relation to the average initial thickness of the reagent layer. The biosensor system determines the analyte concentration from the output signal having a transient decay.

摘要翻译： 生物传感器系统使用没有Cottrell衰变的电化学过程来确定生物样品的分析物浓度。 生物传感器系统产生具有瞬时衰减的输出信号，其中输出信号不与时间的平方根成反比。 瞬态衰减大于或小于Cottrell衰变的-0.5衰减常数。 瞬时衰减可能由相对较短的潜伏期，相对小的样品储存容量，电极表面与传感器条的盖之间的相对小的距离和/或相对于试剂层的平均初始厚度的相对短的激发而产生。 生物传感器系统从具有瞬时衰减的输出信号确定分析物浓度。

公开(公告)号：US20190106728A1

公开(公告)日：2019-04-11

申请号：US16221017

申请日：2018-12-14

申请人： Huan-Ping Wu ,  Steven C. Charlton ,  Amy H. Chu ,  Andrew J. Edelbrock ,  Sung-Kwon Jung ,  Dijia Huang

发明人： Huan-Ping Wu ,  Steven C. Charlton ,  Amy H. Chu ,  Andrew J. Edelbrock ,  Sung-Kwon Jung ,  Dijia Huang

IPC分类号： C12Q1/54 ,  G01N27/327 ,  C12Q1/00 ,  A61B5/1486 ,  A61B5/145

摘要： A biosensor system determines an analyte concentration of a biological sample using an electrochemical process without Cottrell decay. The biosensor system generates an output signal having a transient decay, where the output signal is not inversely proportional to the square root of the time. The transient decay is greater or less than the −0.5 decay constant of a Cottrell decay. The transient decay may result from a relatively short incubation period, relatively small sample reservoir volumes, relatively small distances between electrode surfaces and the lid of the sensor strip, and/or relatively short excitations in relation to the average initial thickness of the reagent layer. The biosensor system determines the analyte concentration from the output signal having a transient decay.

公开(公告)号：US20120031776A1

公开(公告)日：2012-02-09

申请号：US13210738

申请日：2011-08-16

申请人： Huan-Ping Wu ,  Steven C. Charlton ,  Amy H. Chu ,  Andrew J. Edelbrock ,  Sung-Kwon Jung ,  Dijia Huang

发明人： Huan-Ping Wu ,  Steven C. Charlton ,  Amy H. Chu ,  Andrew J. Edelbrock ,  Sung-Kwon Jung ,  Dijia Huang

IPC分类号： G01N27/333 ,  G01N33/66

CPC分类号： C12Q1/54 ,  A61B5/14532 ,  A61B5/1455 ,  A61B5/1486 ,  A61B5/1495 ,  C12Q1/004 ,  G01N27/3271 ,  G01N27/3272

摘要： A biosensor system determines an analyte concentration of a biological sample using an electrochemical process without Cottrell decay. The biosensor system generates an output signal having a transient decay, where the output signal is not inversely proportional to the square root of the time. The transient decay is greater or less than the −0.5 decay constant of a Cottrell decay. The transient decay may result from a relatively short incubation period, relatively small sample reservoir volumes, relatively small distances between electrode surfaces and the lid of the sensor strip, and/or relatively short excitations in relation to the average initial thickness of the reagent layer. The biosensor system determines the analyte concentration from the output signal having a transient decay.

摘要翻译： 生物传感器系统使用没有Cottrell衰变的电化学过程来确定生物样品的分析物浓度。 生物传感器系统产生具有瞬时衰减的输出信号，其中输出信号不与时间的平方根成反比。 瞬态衰减大于或小于Cottrell衰变的-0.5衰减常数。 瞬时衰减可能由相对较短的潜伏期，相对小的样品储存容量，电极表面与传感器条的盖之间的相对小的距离和/或相对于试剂层的平均初始厚度的相对短的激发而产生。 生物传感器系统从具有瞬时衰减的输出信号确定分析物浓度。

公开(公告)号：US08026104B2

公开(公告)日：2011-09-27

申请号：US11875942

申请日：2007-10-21

申请人： Huan-Ping Wu ,  Steven C. Charlton ,  Amy H. Chu ,  Andrew J. Edelbrock ,  Sung-Kwon Jung ,  Dijia Huang

发明人： Huan-Ping Wu ,  Steven C. Charlton ,  Amy H. Chu ,  Andrew J. Edelbrock ,  Sung-Kwon Jung ,  Dijia Huang

IPC分类号： G01N27/00 ,  G01N27/413

CPC分类号： C12Q1/54 ,  A61B5/14532 ,  A61B5/1455 ,  A61B5/1486 ,  A61B5/1495 ,  C12Q1/004 ,  G01N27/3271 ,  G01N27/3272

摘要： A biosensor system determines an analyte concentration of a biological sample using an electrochemical process without Cottrell decay. The biosensor system generates an output signal having a transient decay, where the output signal is not inversely proportional to the square root of the time. The transient decay is greater or less than the −0.5 decay constant of a Cottrell decay. The transient decay may result from a relatively short incubation period, relatively small sample reservoir volumes, relatively small distances between electrode surfaces and the lid of the sensor strip, and/or relatively short excitations in relation to the average initial thickness of the reagent layer. The biosensor system determines the analyte concentration from the output signal having a transient decay.

摘要翻译： 生物传感器系统使用没有Cottrell衰变的电化学过程来确定生物样品的分析物浓度。 生物传感器系统产生具有瞬时衰减的输出信号，其中输出信号不与时间的平方根成反比。 瞬态衰减大于或小于Cottrell衰变的-0.5衰减常数。 瞬时衰减可能由相对较短的潜伏期，相对小的样品储存容量，电极表面与传感器条的盖之间的相对小的距离和/或相对于试剂层的平均初始厚度的相对短的激发而产生。 生物传感器系统从具有瞬时衰减的输出信号确定分析物浓度。

公开(公告)号：US20130334066A1

公开(公告)日：2013-12-19

申请号：US13904208

申请日：2013-05-29

申请人： Huan-Ping Wu ,  Steven C. Charlton ,  Amy H. Chu ,  Andrew J. Edelbrock ,  Sung-Kwon Jung ,  Dijia Huang

发明人： Huan-Ping Wu ,  Steven C. Charlton ,  Amy H. Chu ,  Andrew J. Edelbrock ,  Sung-Kwon Jung ,  Dijia Huang

IPC分类号： G01N27/327

CPC分类号： C12Q1/54 ,  A61B5/14532 ,  A61B5/1455 ,  A61B5/1486 ,  A61B5/1495 ,  C12Q1/004 ,  G01N27/3271 ,  G01N27/3272

摘要： A biosensor system determines an analyte concentration of a biological sample using an electrochemical process without Cottrell decay. The biosensor system generates an output signal having a transient decay, where the output signal is not inversely proportional to the square root of the time. The transient decay is greater or less than the −0.5 decay constant of a Cottrell decay. The transient decay may result from a relatively short incubation period, relatively small sample reservoir volumes, relatively small distances between electrode surfaces and the lid of the sensor strip, and/or relatively short excitations in relation to the average initial thickness of the reagent layer. The biosensor system determines the analyte concentration from the output signal having a transient decay.

摘要翻译： 生物传感器系统使用没有Cottrell衰变的电化学过程来确定生物样品的分析物浓度。 生物传感器系统产生具有瞬时衰减的输出信号，其中输出信号不与时间的平方根成反比。 瞬态衰减大于或小于Cottrell衰变的-0.5衰减常数。 瞬时衰减可能由相对较短的潜伏期，相对小的样品储存容量，电极表面与传感器条的盖之间的相对小的距离和/或相对于试剂层的平均初始厚度的相对短的激发而产生。 生物传感器系统从具有瞬时衰减的输出信号确定分析物浓度。

公开(公告)号：US09164076B2

公开(公告)日：2015-10-20

申请号：US13117872

申请日：2011-05-27

申请人： Dijia Huang ,  Huan-Ping Wu

发明人： Dijia Huang ,  Huan-Ping Wu

IPC分类号： G01N33/48 ,  G01N33/487

CPC分类号： G01N27/3274 ,  G01N33/48714

摘要： A biosensor system determines analyte concentration from analytic and/or secondary output signals. The biosensor system adjusts a correlation for determining analyte concentrations from output signals with one or more index functions extracted from the output signals. The index functions determine at least one slope deviation or normalized slope deviation from one or more error parameters. The slope-adjusted correlation between analyte concentrations and output signals may be used to determine analyte concentrations having improved accuracy and/or precision from output signals including components attributable to bias.

摘要翻译： 生物传感器系统从分析和/或次要输出信号确定分析物浓度。 生物传感器系统通过从输出信号提取的一个或多个指数函数来调整从输出信号确定分析物浓度的相关性。 索引函数确定与一个或多个错误参数的至少一个斜率偏差或归一化斜率偏差。 可以使用分析物浓度和输出信号之间的斜率调整的相关性来确定包括归因于偏压的成分的输出信号具有改进的精度和/或精度的分析物浓度。

公开(公告)号：US09034160B2

公开(公告)日：2015-05-19

申请号：US13401222

申请日：2012-02-21

申请人： Huan-Ping Wu

发明人： Huan-Ping Wu

IPC分类号： G01N27/26 ,  G01N33/487 ,  G01N33/50 ,  C12Q1/00 ,  G01N27/327 ,  G01N27/406 ,  G01N35/00

CPC分类号： G01N27/327 ,  G01N27/3273 ,  G01N27/4065 ,  G01N35/0092

摘要： A sensor system, device, and methods for determining the concentration of an analyte in a sample is described. Input signals including multiple duty cycles of sequential excitation pulses and relaxations are input to the sample. One or more signals output from the sample within 300 ms of the input of an excitation pulse may be correlated with the analyte concentration of the sample to improve the accuracy and/or precision of the analysis. Determining the analyte concentration of the sample from these rapidly measured output values may reduce analysis errors arising from the hematocrit effect, mediator background, and other error sources.

摘要翻译： 描述了用于确定样品中分析物浓度的传感器系统，装置和方法。 包括连续激励脉冲和松弛的多个占空比的输入信号被输入到样品。 在激励脉冲的输入的300ms内从样品输出的一个或多个信号可以与样品的分析物浓度相关联，以提高分析的精度和/或精度。 从这些快速测量的输出值确定样品的分析物浓度可能会降低由血细胞比容影响，介质背景和其他误差源引起的分析误差。

公开(公告)号：US08973422B2

公开(公告)日：2015-03-10

申请号：US13107363

申请日：2011-05-13

申请人： Huan-Ping Wu ,  Christine D. Nelson

发明人： Huan-Ping Wu ,  Christine D. Nelson

IPC分类号： G01N27/327 ,  G01N27/26 ,  G01N35/00 ,  G01N35/10

CPC分类号： G01N27/4163 ,  G01N27/327 ,  G01N27/3274 ,  G01N35/00029 ,  G01N35/1016 ,  G01N2035/00108 ,  Y10T436/10

摘要： A biosensor has an underfill detection system that determines whether a sample of a biological fluid is large enough for an analysis of one or more analytes. The underfill detection system applies an excitation signal to the sample, which generates an output signal in response to the excitation signal. The underfill detection system switches the amplitude of the excitation signal. The transition of the excitation signal to a different amplitude changes the output signal when the sample is not large enough for an accurate and/or precise analysis. The underfill detection system measures and compares the output signal with one or more underfill thresholds to determine whether an underfill condition exists.

摘要翻译： 生物传感器具有底部填充检测系统，其确定生物流体的样品是否足够大以分析一种或多种分析物。 底部填充检测系统向样品施加激励信号，其产生响应于激发信号的输出信号。 底部填充检测系统切换激励信号的幅度。 当样品不够大以进行准确和/或精确的分析时，激发信号向不同幅度的转变会改变输出信号。 底部填充检测系统测量并比较输出信号与一个或多个底部填充阈值，以确定是否存在底部填充条件。