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公开(公告)号：US08283524B2
公开(公告)日：2012-10-09
申请号：US12436171
申请日：2009-05-06
申请人： Andre R. Abad , Hua Dong , Susan B. Lo , Mark Nelson , Xiaomei Shi , Clement W. K. Waine , Takashi Yamamoto
发明人： Andre R. Abad , Hua Dong , Susan B. Lo , Mark Nelson , Xiaomei Shi , Clement W. K. Waine , Takashi Yamamoto
CPC分类号： C12N15/8286 , C07K14/32 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
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公开(公告)号：US20100269221A1
公开(公告)日：2010-10-21
申请号：US12436171
申请日：2009-05-06
申请人： Andre R. Abad , Hua Dong , Susan B. Lo , Mark Nelson , Xiaomei Shi , Clement W.K. Waine , Takashi Yamamoto
发明人： Andre R. Abad , Hua Dong , Susan B. Lo , Mark Nelson , Xiaomei Shi , Clement W.K. Waine , Takashi Yamamoto
IPC分类号： A01H1/00 , A01H5/00 , C12N15/82 , C07H21/04 , C12N1/21 , C12N5/10 , C12N15/63 , C12N15/79 , C07K14/00 , A01N61/00 , C12P21/02 , A01P7/04
CPC分类号： C12N15/8286 , C07K14/32 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
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公开(公告)号：US08148077B2
公开(公告)日：2012-04-03
申请号：US11780884
申请日：2007-07-20
申请人： Andre R. Abad , Hua Dong , Susan B. Lo , Billy F. McCutchen , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Susan B. Lo , Billy F. McCutchen , Xiaomei Shi
IPC分类号： C12P19/34
CPC分类号： C12N15/8286 , C12Q1/6813 , Y02A40/162 , C12Q2531/113
摘要： Methods and compositions for identifying novel genes that share regions of homology with known genes from target groups of genes of interest are provided. The methods comprise systematically designing oligonucleotide primers that are specific for regions of homology within the nucleotide sequences of a target group of known genes and performing successive rounds of PCR amplification of nucleic acid material from an organism of interest. The PCR steps are intended to identify and amplify nucleic acids comprising both known and novel genes. Nucleic acid molecules comprising known genes are detected and eliminated from further consideration by dot blot analysis using oligonucleotide probes specific for the known genes in the target group. Potentially novel genes are subjected to further sequence analysis to confirm novelty and assayed for biological activity. Compositions of the present invention include novel polynucleotides, and variants and fragments thereof, that comprise novel genes and the polypeptides encoded thereby.
摘要翻译： 提供了用于鉴定与目标基因组的已知基因具有同源性区域的新基因的方法和组合物。 所述方法包括系统地设计对已知基因的靶组的核苷酸序列内的同源区特异性的寡核苷酸引物，并且从感兴趣的生物体进行连续循环的核酸材料的PCR扩增。 PCR步骤旨在鉴定和扩增包含已知和新基因的核酸。 通过使用针对靶组中已知基因特异的寡核苷酸探针的斑点印迹分析，进一步考虑检测并消除包含已知基因的核酸分子。 将潜在的新基因进行进一步的序列分析以证实新颖性并测定生物活性。 本发明的组合物包括新型多核苷酸及其变体和片段，其包含新基因和由其编码的多肽。
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公开(公告)号：US08878007B2
公开(公告)日：2014-11-04
申请号：US13413707
申请日：2012-03-07
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi , Thomas C. Wolfe
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi , Thomas C. Wolfe
CPC分类号： C12N15/8279 , A01N63/02 , C07K14/325
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
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公开(公告)号：US08319019B2
公开(公告)日：2012-11-27
申请号：US12616298
申请日：2009-11-11
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
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公开(公告)号：US08129594B2
公开(公告)日：2012-03-06
申请号：US12477393
申请日：2009-06-03
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
CPC分类号： C12N15/8286 , C07K14/32 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
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7.发明授权公开(公告)号：US07812129B1
公开(公告)日：2010-10-12
申请号：US12402637
申请日：2009-03-12
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译： 本发明提供从编码具有杀虫活性的昆虫的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其包括鳞翅目昆虫害虫。 本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，DNA构建体和转化的微生物的分离的核酸和包含实施方案的核酸的植物。 这些组合物可用于防治害虫，特别是植物害虫的方法。
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公开(公告)号：US08933299B2
公开(公告)日：2015-01-13
申请号：US13211466
申请日：2011-08-17
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
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公开(公告)号：US08802934B2
公开(公告)日：2014-08-12
申请号：US13209473
申请日：2011-08-15
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi , Thomas C. Wolfe
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi , Thomas C. Wolfe
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
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公开(公告)号：US20120297503A1
公开(公告)日：2012-11-22
申请号：US13555393
申请日：2012-07-23
申请人： Andre R. Abad , Hua Dong , Deirdre M. Kapka-Kitzman , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Deirdre M. Kapka-Kitzman , Sue B. Lo , Xiaomei Shi
IPC分类号： C07K14/32 , C12N1/21 , C12N5/10 , A01H1/06 , A01N37/46 , A01P7/04 , C12P21/02 , A01H5/00 , C12N15/31 , A01H5/10
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
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