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    MOLECULAR ARCHITECTURE ON MAGNETIC PARTICLES FOR AFFINITY ASSAYS WITH LOW NON-SPECIFIC BINDING
    1.
    发明申请
    MOLECULAR ARCHITECTURE ON MAGNETIC PARTICLES FOR AFFINITY ASSAYS WITH LOW NON-SPECIFIC BINDING 审中-公开
    具有低非特异性结合的亲和力测定用磁性颗粒的分子结构

    公开(公告)号：US20140127722A1

    公开(公告)日：2014-05-08

    申请号：US14128031

    申请日：2012-06-26

    申请人： Andrea Ranzoni ,  Gwenola Sabatte ,  Menno Willem Jose Prins

    发明人： Andrea Ranzoni ,  Gwenola Sabatte ,  Menno Willem Jose Prins

    IPC分类号： G01N33/543

    CPC分类号： G01N33/54333 ,  G01N33/54326 ,  G01N33/54393

    摘要： The present invention relates to a coating for preventing non-specific binding of molecules to a surface layer comprising an affinity molecule, said coating comprises single layer comprising non-affine spacer molecules forming a mesh; or a shell structure, wherein said shell structure comprises a first layer comprising one or more affinity molecules and further a second layer, which is coupled to the first layer, and wherein said first and second layer comprise non-affine spacer molecules forming a mesh. The present invention further relates to a particle, a flat structure or a matrix comprising such a coating. In a further aspect the present invention relates to the use of the coating, coated particle, coated flat structure or coated matrix for detection and/or purification of a specific target biomolecule from a sample and/or determination of the concentration of the specific target biomolecule. The present invention further relates to an assay for the detection of specific target biomolecules from a sample and/or determination of the concentration of specific target biomolecules comprising the use of such coating, coated particle, coated flat structure or coated matrix, in particular coated nanoparticles.

    摘要翻译： 本发明涉及一种用于防止分子与包含亲和分子的表面层的非特异性结合的涂层，所述涂层包括单层，其包含形成网的非仿射间隔分子; 或壳结构，其中所述壳结构包括包含一个或多个亲和分子的第一层，以及第二层，其耦合到第一层，并且其中所述第一层和第二层包含形成网的非仿射间隔分子。 本发明还涉及包含这种涂层的颗粒，平面结构或基体。 在另一方面，本发明涉及涂层，包被颗粒，涂覆的扁平结构或包被基质用于从样品中检测和/或纯化特异性目标生物分子和/或确定特异性靶标生物分子浓度的用途 。 本发明还涉及用于从样品中检测特异性目标生物分子和/或确定特异性靶生物分子的浓度的测定法，其包括使用这种涂层，涂覆的颗粒，涂覆的平面结构或涂覆的基质，特别是涂覆的纳米颗粒 。

    MULTI EPITOPE ASSAY
    3.
    发明申请
    MULTI EPITOPE ASSAY 有权
    多重抗生素测定

    公开(公告)号：US20130095495A1

    公开(公告)日：2013-04-18

    申请号：US13704258

    申请日：2011-06-14

    申请人： Gwenola Sabatte ,  Menno Willem Jose Prins ,  Toon Hendrik Evers ,  Wilhelmina Maria Hardeman ,  Joukje Garrelina Orsel

    发明人： Gwenola Sabatte ,  Menno Willem Jose Prins ,  Toon Hendrik Evers ,  Wilhelmina Maria Hardeman ,  Joukje Garrelina Orsel

    IPC分类号： G01N33/53

    CPC分类号： G01N33/5306 ,  G01N33/54306 ,  G01N33/54333

    摘要： The present invention is related to a method for detection of a biological target in an affinity assay, the method comprising the steps of providing a biological sample volume containing the biological target, adding a first capturing moiety to the biological sample volume comprising the biological target, wherein the first capturing moiety is adhered to a particle, concentration of the captured biological target into an elution volume that is smaller than the biological sample volume in step a), cleavage of the first capturing moiety or the biological target from the particle and direct or indirect detection and/or quantification of the biological target in a sandwich or competitive affinity assay format, wherein the biological target is associated with at least one capturing moiety, preferably at least two capturing moieties.

    摘要翻译： 本发明涉及亲和测定中生物靶标检测方法，该方法包括以下步骤：提供含生物靶的生物样品体积，向包含生物靶标的生物样品体积添加第一捕获部分， 其中所述第一捕获部分被粘附到颗粒上，所捕获的生物靶的浓度在步骤a）中小于生物样品体积的洗脱体积，从所述颗粒切割所述第一捕获部分或所述生物靶，并直接或 间接检测和/或以三明治或竞争亲和测定格式定量生物学靶标，其中生物靶标与至少一个捕获部分，优选至少两个捕获部分相关联。

    ROTATING MAGNETIC FIELD FOR IMPROVED DETECTION IN CLUSTER ASSAYS
    4.
    发明申请
    ROTATING MAGNETIC FIELD FOR IMPROVED DETECTION IN CLUSTER ASSAYS 有权
    旋转磁场用于集群测量中的改进检测

    公开(公告)号：US20110156701A1

    公开(公告)日：2011-06-30

    申请号：US13060804

    申请日：2009-09-04

    申请人： Andrea Ranzoni ,  Menno Willem Jose Prins ,  Mikhail Mikhaylovich Ovsyanko

    发明人： Andrea Ranzoni ,  Menno Willem Jose Prins ,  Mikhail Mikhaylovich Ovsyanko

    IPC分类号： G01R33/02 ,  C40B30/00

    CPC分类号： G01N33/54333 ,  G01N27/745

    摘要： In a method of performing a cluster assay, a suspension (14) of superparamagnetic particles in a fluid to be analyzed is provided, wherein the superparamagnetic particles are coated with a bioactive agent. The particles are then allowed to form clusters due to an analyte present within the fluid. Subsequently, clusters of superparamagnetic particles are selectively actuated by applying a rotating magnetic field, wherein the amplitude of the magnetic field varies over time. Finally, the selectively actuated clusters are detected. An apparatus for performing a cluster assay comprises means for accommodating a sample (12) and means for applying a rotating magnetic field (11), the magnetic field being adapted for selectively actuating clusters of superparamagnetic particles. The apparatus further comprises means for detecting the selectively actuated clusters.

    摘要翻译： 在进行聚集测定的方法中，提供了待分析流体中超顺磁性颗粒的悬浮液（14），其中超顺磁性颗粒用生物活性剂涂覆。 然后由于存在于流体内的分析物，使颗粒形成簇。 随后，通过施加旋转磁场来选择性地致动超顺磁性颗粒簇，其中磁场的振幅随时间而变化。 最后，检测到选择性地致动的簇。 用于进行聚集测定的装置包括用于容纳样品（12）的装置和用于施加旋转磁场（11）的装置，所述磁场适于选择性地致动超顺磁颗粒簇。 该装置还包括用于检测选择性致动的簇的装置。

    Rotating magnetic field for improved detection in cluster assays
    5.
    发明授权
    Rotating magnetic field for improved detection in cluster assays 有权
    旋转磁场用于集群测定中的改进检测

    公开(公告)号：US08981772B2

    公开(公告)日：2015-03-17

    申请号：US13060804

    申请日：2009-09-04

    申请人： Andrea Ranzoni ,  Menno Willem Jose Prins ,  Mikhail Mikhaylovich Ovsyanko

    发明人： Andrea Ranzoni ,  Menno Willem Jose Prins ,  Mikhail Mikhaylovich Ovsyanko

    IPC分类号： G01R33/02 ,  G01N33/553 ,  G01N33/543 ,  G01N27/74

    CPC分类号： G01N33/54333 ,  G01N27/745

    摘要： In a method of performing a cluster assay, a suspension (14) of superparamagnetic particles in a fluid to be analyzed is provided, wherein the superparamagnetic particles are coated with a bioactive agent. The particles are then allowed to form clusters due to an analyte present within the fluid. Subsequently, clusters of superparamagnetic particles are selectively actuated by applying a rotating magnetic field, wherein the amplitude of the magnetic field varies over time. Finally, the selectively actuated clusters are detected. An apparatus for performing a cluster assay comprises means for accommodating a sample (12) and means for applying a rotating magnetic field (11), the magnetic field being adapted for selectively actuating clusters of superparamagnetic particles. The apparatus further comprises means for detecting the selectively actuated clusters.

    摘要翻译： 在进行聚集测定的方法中，提供了待分析流体中超顺磁性颗粒的悬浮液（14），其中超顺磁性颗粒用生物活性剂涂覆。 然后由于存在于流体内的分析物，使颗粒形成簇。 随后，通过施加旋转磁场来选择性地致动超顺磁性颗粒簇，其中磁场的振幅随时间而变化。 最后，检测到选择性地致动的簇。 用于进行聚集测定的装置包括用于容纳样品（12）的装置和用于施加旋转磁场（11）的装置，所述磁场适于选择性地致动超顺磁颗粒簇。 该装置还包括用于检测选择性地致动的簇的装置。

    DETECTION OF CLUSTERS OF MAGNETIC PARTICLES
    7.
    发明申请
    DETECTION OF CLUSTERS OF MAGNETIC PARTICLES 审中-公开
    检测磁性颗粒群

    公开(公告)号：US20140120632A1

    公开(公告)日：2014-05-01

    申请号：US14125618

    申请日：2012-06-18

    申请人： Andrea Ranzoni ,  Menno Willem Jose Prins

    发明人： Andrea Ranzoni ,  Menno Willem Jose Prins

    IPC分类号： G01N27/74

    CPC分类号： G01N27/745 ,  G01N21/1717 ,  G01N21/51 ,  G01N2021/1727 ,  G01N2446/00

    摘要： The invention relates to a method and a sensor device (100) for the detection of clusters (C) of magnetic particles (MP) in a sample volume (111), particularly of clusters (C) consisting of two magnetic particles (MP) with different binding sites that are bound to a target molecule in a sandwich configuration. Output light (L2) originating from an interaction of input light (L1) with clusters (C) of magnetic particles (MP) is detected. Moreover, the magnetic particles (MP, C) are actuated by a magnetic actuation field (B), wherein said actuation is at least once interrupted by a pause. In this way a high output signal can be achieved that properly reflects the amount of specifically bound clusters (C).

    摘要翻译： 本发明涉及一种用于检测样品体积（111）中的磁性颗粒（MP）的簇（C）的方法和传感器装置（100），特别是用两个磁性颗粒（MP）组成的簇（C） 与夹层结构中的靶分子结合的不同结合位点。 检测源自输入光（L1）与磁性粒子（MP）的簇（C）的相互作用的输出光（L2）。 此外，磁性颗粒（MP，C）由磁性致动场（B）致动，其中所述致动至少一次被暂停中断。 以这种方式，可以实现适当地反映特定结合簇（C）的量的高输出信号。

    DETECTION OF DIFFERENT TARGET COMPONENTS BY CLUSTER FORMATION
    8.
    发明申请
    DETECTION OF DIFFERENT TARGET COMPONENTS BY CLUSTER FORMATION 审中-公开
    通过集群形成检测不同的目标组件

    公开(公告)号：US20120208296A1

    公开(公告)日：2012-08-16

    申请号：US13391108

    申请日：2010-08-16

    申请人： Andrea Ranzoni ,  Mikhail Mikhaylovich Ovsyanko ,  Menno Willem Jose Prins

    发明人： Andrea Ranzoni ,  Mikhail Mikhaylovich Ovsyanko ,  Menno Willem Jose Prins

    IPC分类号： G01N27/00 ,  G01N21/75

    CPC分类号： G01N33/54333 ,  G01N27/745 ,  G01N33/54306 ,  G01N33/54366

    摘要： The invention relates to a method, a test kit, and an apparatus (100) for detecting a plurality of different species of target components (T1, T2) in a sample. This is achieved by using a plurality of classes of label particles (1, 2), wherein at least one of the label particles (1, 2) in each class is a magnetic particle, and wherein label particles (1, 2) from each class can bind to each other via the same class-specific species of target component. Clusters of label particles (1, 2) can then form in which binding to specific target components (T1, T2) is accompanied by characteristic properties, for example magnetic susceptibilities of the associated label particles (1, 2). The selective actuation of such clusters by a magnetic field (B) with at least one oscillating component and a variable field amplitude together with the detection of such selectively actuated clusters will hence allow to specifically detect the clusters. This provides information about the different target components (T1, T2) in the sample.

    摘要翻译： 本发明涉及用于检测样品中多种不同种类的目标成分（T1，T2）的方法，测试试剂盒和装置（100）。 这通过使用多种类型的标签颗粒（1,2）来实现，其中每个类别中的标签颗粒（1,2）中的至少一个是磁性颗粒，并且其中来自每个的标签颗粒（1,2） 类可以通过目标组件的相同类特定物种彼此结合。 然后可以形成与特定靶组分（T1，T2）结合的标记颗粒（1,2）簇，其特征在于特征性质，例如相关标记颗粒（1,2）的磁化率。 具有至少一个振荡分量和可变场振幅的磁场（B）对这种簇的选择性驱动与这种选择性致动的簇的检测一起将因此允许特定地检测簇。 这提供了有关样本中不同目标组件（T1，T2）的信息。