公开(公告)号：US06905851B1

公开(公告)日：2005-06-14

申请号：US10030726

申请日：2000-06-29

申请人： Antónia Jekkel ,  Gábor Ambrus ,  Éva Ilkóy ,  Ildikó Horváth ,  Attila Kónya ,  István Mihály Szabó ,  Zsuzsanna Nagy ,  Gyula Horváth ,  Júlia Mózes ,  István Barta ,  György Somogyi ,  János Salát ,  Sándor Boros

发明人： Antónia Jekkel ,  Gábor Ambrus ,  Éva Ilkóy ,  Ildikó Horváth ,  Attila Kónya ,  István Mihály Szabó ,  Zsuzsanna Nagy ,  Gyula Horváth ,  Júlia Mózes ,  István Barta ,  György Somogyi ,  János Salát ,  Sándor Boros

IPC分类号： C12P7/62 ,  C07C69/30 ,  C12P1/04 ,  C12P7/42 ,  C12P17/06 ,  C12R1/29 ,  C12R1/31 ,  C12P17/42

CPC分类号： C12R1/29 ,  C12P7/42 ,  C12P17/06

摘要： The present invention relates to a new microbial process for the preparation of compound of formula (I) from a compound of general formula (II) wherein R stands for an alkali metal or ammonium ion, by the submerged culture of a strain which is able to 6β-hydroxylate the compound of formula (II) in aerobic fermentation and by the separation and purification of the product of formula (I) formed in the course of the bioconversion. The latter comprises the cultivation of a Micromonospora strain which is able to 6β-hydroxylate a compound of general formula (II)—wherein R is as defined above—at 25-32° C. on a nutrient medium containing available carbon—and nitrogen sources and mineral salts, thereafter feeding the substrate to be transformed into the developing culture, then hydroxilating the substrate until finishing of the bioconversion, then separating the compound of formula (I) from the culture broth and, if desired, purifying the same.

摘要翻译： 本发明涉及一种由通式（II）化合物制备式（I）化合物的新型微生物方法，其中R代表碱金属或铵离子，通过浸没培养的能够 在好氧发酵中通过分解和纯化在生物转化过程中形成的式（I）的产物，使式（II）化合物6β-羟基化。 后者包括培养微孢子虫菌株，其能够在含有可用碳源和氮源的营养培养基上在25-32℃下将通式（II）化合物（其中R如上定义）6β-羟基化 然后将待转化的底物喂入显影培养物，然后将底物氢化，直到生物转化完成，然后从培养液中分离式（I）化合物，如果需要，将其纯化。