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公开(公告)号：US5508197A
公开(公告)日：1996-04-16
申请号：US279412
申请日：1994-07-25
CPC分类号： B01L7/52 , B01L2300/0893 , Y10S435/809
摘要： A thermal cycling system and method of use are described. The thermal cycling system is based on the-circulation of temperature-controlled water directly to the underside of thin-walled polycarbonate microtiter plates. The water flow is selected from a manifold fed by pumps from heated reservoirs. The plate wells are loaded with typically 15-20 .mu.l of reagent mix for the PCR process. Heat transfer through the thin polycarbonate is sufficiently rapid that the contents reach thermal equilibrium with the water in less than 15 seconds. Complete PCR amplification runs of 40 three-step cycles have been performed in as little as 14.5 minutes, with the results showing substantially enhanced specificity compared to conventional technology requiring run times in excess of 100 minutes. The plate clamping station is designed to be amenable to robotic loading and unloading of the system. It includes a heated lid, thus eliminating the need for mineral oil overlay of the reactants. The present system includes three or more plate holder stations, fed from common reservoirs but operating with independent switching cycles. The system can be modularly expanded.
摘要翻译： 描述了一种热循环系统和使用方法。 热循环系统基于将温度控制的水直接循环到薄壁聚碳酸酯微量滴定板的底部。 水流从加热储存器的泵供给的歧管中选择。 加载平板孔通常用于PCR过程的15-20μl试剂混合物。 通过薄聚碳酸酯的热传递足够快，使得内容物在少于15秒内与水达到热平衡。 在短短14.5分钟内进行了40个三步循环的完全PCR扩增，结果显示出与需要超过100分钟的运行时间的常规技术相比，显着提高了特异性。 夹板站设计为适合于系统的机器人装载和卸载。 它包括加热盖，因此不需要反应物的矿物油覆盖。 本系统包括三个或更多个板夹持器站，其由公共储存器供给，但是以独立的开关周期运行。 该系统可以模块化地扩展。
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公开(公告)号：US08411272B2
公开(公告)日：2013-04-02
申请号：US12534343
申请日：2009-08-03
申请人： Anthony D. A. Hansen
发明人： Anthony D. A. Hansen
CPC分类号： G01N21/4738 , G01N15/0625 , G01N21/59
摘要： An apparatus and method are presented for the analysis of materials. The apparatus includes two or more similar analyzers, with the output of the analyzers combined to provide improved measurements. The apparatus may be, for example, a differential photometric analyzer, such as the AETHALOMETER®. The apparatus and method includes providing flows to the analyzers such that the rate of accumulation per filter area differs for the two or more analyzers. The output of the apparatus or method may be a concentration, such as the concentration of black carbon particulates. Additionally, the output may be an optical measure of particulates that is useful for characterizing the source or history of the particulates.
摘要翻译： 提出了一种用于材料分析的装置和方法。 该装置包括两个或更多类似的分析器，其中分析器的输出组合以提供改进的测量。 该装置可以是例如差示光度分析仪，例如AETHALOMETER。 该装置和方法包括向分析器提供流量，使得对于两个或更多个分析器，每个过滤区域的累积速率不同。 装置或方法的输出可以是浓度，例如黑碳颗粒的浓度。 此外，输出可以是用于表征颗粒的来源或历史的微粒的光学测量。
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3.发明授权公开(公告)号：US08531671B1
公开(公告)日：2013-09-10
申请号：US12960344
申请日：2010-12-03
申请人： Anthony D. A. Hansen
发明人： Anthony D. A. Hansen
CPC分类号： G01N1/2205 , G01N1/2252 , G01N15/0625 , G01N2001/2255 , G01N2001/227 , G01N2015/0046
摘要： Apparatus and methods are described for determining particulate concentrations in an exhaust gas. One embodiment provides a method of analyzing particles in a gas using an apparatus including a probe and an analyzer. The method includes obtaining a sample of particle laden gas through the probe; obtaining ambient air through the probe; diluting the sample with the ambient air; and providing the diluted sample to the analyzer. Another embodiment provides an apparatus for analyzing particles in a gas. The apparatus includes a probe adapted to accept a gas or ambient air; a volume to dilute the gas with the ambient air; an analyzer responsive to particles in the gas; and electronics to operate the apparatus. The electronics instructs a user to first provide a sample and then provide ambient air into the volume, and then analyzes substantially all of the sample and ambient air for particles.
摘要翻译： 描述了用于确定废气中的颗粒浓度的装置和方法。 一个实施例提供了一种使用包括探针和分析器的装置分析气体中的颗粒的方法。 该方法包括通过探针获得载有颗粒的气体样品; 通过探头获得环境空气; 用环境空气稀释样品; 并将稀释的样品提供给分析仪。 另一个实施例提供一种用于分析气体中的颗粒的装置。 该装置包括适于接收气体或环境空气的探针; 用环境空气稀释气体的体积; 响应于气体中的颗粒的分析仪; 和电子设备来操作设备。 电子装置指示用户首先提供样品，然后向体积中提供环境空气，然后基本上分析所有样品和环境空气的颗粒。
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4.发明授权公开(公告)号：US09018583B2
公开(公告)日：2015-04-28
申请号：US13413580
申请日：2012-03-06
CPC分类号： G01N15/1012 , G01N15/0618 , G01N15/0625 , G01N21/274 , G01N21/59 , G01N2201/0627
摘要： A method is provided for diagnosing the operation of a photometric particle analyzer. The method may determine when the operation is degraded from normal operating conditions, automatically, and the result displayed locally as well as being transmitted to a remote observer. The present invention may be used by optical photometric particle analyzers, or by analyzers that measure other properties of particles collected on filters.
摘要翻译： 提供了用于诊断光度粒子分析仪的操作的方法。 该方法可以确定操作何时自动地从正常操作条件降级，并且本地显示的结果以及被发送到远程观察者。 本发明可以由光学测微粒子分析仪，或测量滤器上收集的颗粒的其它特性的分析仪使用。
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公开(公告)号：US08919402B2
公开(公告)日：2014-12-30
申请号：US12951369
申请日：2010-11-22
申请人： Anthony D. A. Hansen
发明人： Anthony D. A. Hansen
IPC分类号： B60C23/12
CPC分类号： B60C23/12
摘要： An apparatus and method are described for extracting useful work from pressure transients in a pneumatic tire to replace lost air from the tire. The apparatus and method operate continuously and without human intervention. When also used with a pressure relief valve, the apparatus and method permit the tires that are being driven to be maintained at a desired average pressure.
摘要翻译： 描述了一种用于从充气轮胎中的压力瞬变提取有用工作以替换来自轮胎的空气的装置和方法。 该装置和方法连续工作，无需人为干预。 当还与减压阀一起使用时，装置和方法允许被驱动的轮胎保持在期望的平均压力。
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公开(公告)号：US20100027013A1
公开(公告)日：2010-02-04
申请号：US12534343
申请日：2009-08-03
申请人： Anthony D. A. Hansen
发明人： Anthony D. A. Hansen
CPC分类号： G01N21/4738 , G01N15/0625 , G01N21/59
摘要： An apparatus and method are presented for the analysis of materials. The apparatus includes two or more similar analyzers, with the output of the analyzers combined to provide improved measurements. The apparatus may be, for example, a differential photometric analyzer, such as the AETHALOMETER®. The apparatus and method includes providing flows to the analyzers such that the rate of accumulation per filter area differs for the two or more analyzers. The output of the apparatus or method may be a concentration, such as the concentration of black carbon particulates. Additionally, the output may be an optical measure of particulates that is useful for characterizing the source or history of the particulates.
摘要翻译： 提出了一种用于材料分析的装置和方法。 该装置包括两个或更多类似的分析器，其中分析器的输出组合以提供改进的测量。 该装置可以是例如差示光度分析仪，例如AETHALOMETER。 该装置和方法包括向分析器提供流量，使得两个或多个分析器的每个过滤区域的累积速率不同。 装置或方法的输出可以是浓度，例如黑碳颗粒的浓度。 此外，输出可以是用于表征颗粒的来源或历史的微粒的光学测量。
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公开(公告)号：US06317639B1
公开(公告)日：2001-11-13
申请号：US09328054
申请日：1999-06-08
申请人： Anthony D. A. Hansen
发明人： Anthony D. A. Hansen
IPC分类号： G05B1500
摘要： Instrumentation systems and methods for monitoring an environmental condition at a location are disclosed. Embodiments of the invention include wireless communication elements which can transmit pictorial image data from a location being monitored to a server computer. Persons at one or more client computers can view a pictorial image generated from the pictorial image data from any suitable location at any suitable time.
摘要翻译： 公开了用于监测位置处的环境状况的仪表系统和方法。 本发明的实施例包括可将图形图像数据从被监视的位置传送到服务器计算机的无线通信元件。 一个或多个客户端计算机上的人可以在任何合适的时间从任何合适的位置观看从图形图像数据生成的图形图像。
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