公开(公告)号：US20210283253A1

公开(公告)日：2021-09-16

申请号：US16837858

申请日：2018-10-01

申请人： Aridis Pharmaceuticals, Inc.

发明人： Vu Truong-Le

IPC分类号： A61K39/40 ,  C07K16/12 ,  A61K31/496 ,  A61K38/12 ,  A61K31/546 ,  A61K31/427

摘要： A combination of an antibody and other therapeutic that work together in vivo against a pathogenic microbe. The combination can include the antibody with an antibiotic, and/or a therapeutic against a disease. The combination can attack the pathogenic microbe with an efficiency more than either of the components alone, with a synergistic effect, or an effect moderated by one or more modes of action not existing with administration of either the antibody or therapeutic alone.

公开(公告)号：US20240141385A1

公开(公告)日：2024-05-02

申请号：US17811414

申请日：2022-10-27

申请人： Aridis Pharmaceuticals, Inc.

发明人： Kathy Ngo ,  Jennifer Woo ,  Binh Pham ,  Vu Truong-Le

IPC分类号： C12N15/90 ,  C07K16/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/86

CPC分类号： C12N15/907 ,  C07K16/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/111 ,  C12N15/86 ,  C07K2317/14 ,  C12N2310/20 ,  C12N2750/14143 ,  C12N2800/30 ,  C12N2800/80

摘要： This invention provides methods and systems for enhancement of protein production from mammalian cell lines in a drug inducible manner. The methods described herein can be used to generate a protein production cell line wherein the gene coding the protein product of interest is inserted into specific safe harbor loci (SHL) within the cell's genome and the gene copy number is induced to amplify by the use of an antibiotic inducer. The method enables for the conditional activation of the drug inducible transposase. The drug inducible gene amplification method described herein effectively functions as a molecular dial: combining drug-inducible homologous recombination and conditional gene activation to fine-tune gene amplification in mammalian systems.

公开(公告)号：US20240271127A1

公开(公告)日：2024-08-15

申请号：US17784796

申请日：2020-12-15

申请人： Aridis Pharmaceuticals, Inc.

发明人： Kathy Ngo ,  Jennifer Woo ,  Binh Pham ,  Vu Truong-Le

IPC分类号： C12N15/11 ,  C07K16/00 ,  C12N5/071 ,  C12N9/22

CPC分类号： C12N15/111 ,  C07K16/00 ,  C12N5/0682 ,  C12N9/22 ,  C07K2317/14 ,  C12N2310/20

摘要： This application describes cells, systems, and molecular engineering methods using CRISPR/Cas complexes for targeted activation of endogenous master transcriptional regulatory elements (MTRE) such as PRDM1, XBP1 and IRF4, to generate high productivity antibody production in production cell lines such as CHO and NSO cells. These incorporate the inclusion of the Cas accessory proteins, design of multiple guide RNAs (gRNA), and unique multiplexing of these components using, e.g., lentiviral transfection to induce increased transcription and translation of antibody genes under the control of the MTRE. The methods result in synergies increasing monoclonal antibody production by these modified cell lines. While a significant increase in productivity is demonstrated by this method of activation, further increase in productivity can be accomplished by genetic transfer of additional copies of MTREs.

公开(公告)号：US20210322537A1

公开(公告)日：2021-10-21

申请号：US16307314

申请日：2017-06-06

申请人： Aridis Pharmaceuticals, Inc.

发明人： Van Nguyen ,  Phillip M. Lovalenti ,  Vu Truong-Le ,  Jeff Anderl ,  Satoshi Ohtake ,  Atul Saxena

IPC分类号： A61K39/15 ,  A61K9/70 ,  A61P31/14 ,  A61K9/00 ,  A61K47/32 ,  A61K47/10 ,  A61K39/112 ,  A61K47/02

摘要： Methods for the preparation of polymeric films which encase and preserve bioactive agents. In particular, the invention is directed to the preparation of oral thin films containing bioactive proteins or viruses. For example, methods and compositions are disclosed for preservation of rotavirus and antibodies in thin dry films.