公开(公告)号：US20220372586A1

公开(公告)日：2022-11-24

申请号：US17879871

申请日：2022-08-03

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander Green ,  Fan Hong ,  Soma Chaudhary ,  Anli Tang ,  Hao Yan

IPC: C12Q1/6897 ,  C12Q1/6858

Abstract: Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.

公开(公告)号：US20240043853A1

公开(公告)日：2024-02-08

申请号：US18311587

申请日：2023-05-03

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander Green ,  Fan Hong

IPC: C12N15/67 ,  C12Q1/6825 ,  C12Q1/6897

CPC classification number: C12N15/67 ,  C12Q1/6825 ,  C12Q1/6897

Abstract: Provided herein are highly sensitive compositions and methods for detection of at least one specific nucleic acid molecule in a sample. The presence of a specific nucleic acid provides a positive indicator of a pathogenic agent, contaminant, non-canonical bases, and/or wild-type or mutated genes in a sample or a cell. Applications for which the compositions and methods are particularly well suited include point-of-care disease diagnosis or cellular RNA imaging.

公开(公告)号：US11680269B2

公开(公告)日：2023-06-20

申请号：US16322719

申请日：2017-08-01

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander Green ,  Fan Hong

IPC: C12N15/67 ,  C12Q1/6825 ,  C12Q1/6897

CPC classification number: C12N15/67 ,  C12Q1/6825 ,  C12Q1/6897

Abstract: Provided herein are highly sensitive compositions and methods for detection of at least one specific nucleic acid molecule in a sample. The presence of a specific nucleic acid provides a positive indicator of a pathogenic agent, contaminant, non-canonical bases, and/or wild-type or mutated genes in a sample or a cell. Applications for which the compositions and methods are particularly well suited include point-of-care disease diagnosis or cellular RNA imaging.

公开(公告)号：US11434540B2

公开(公告)日：2022-09-06

申请号：US16349752

申请日：2017-11-15

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Alexander Green ,  Fan Hong ,  Soma Chaudhary ,  Anli Tang ,  Hao Yan

IPC: C12Q1/6858 ,  C12Q1/6897

Abstract: Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.

公开(公告)号：US20200071777A1

公开(公告)日：2020-03-05

申请号：US16349752

申请日：2017-11-15

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Alexander Green ,  Fan Hong ,  Soma Chaudhary ,  Anli Tang ,  Hao Yan

IPC: C12Q1/6897 ,  C12Q1/6858

Abstract: Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.