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1.发明申请公开(公告)号：US20190126275A1
公开(公告)日：2019-05-02
申请号：US16095375
申请日：2017-04-21
Inventor： Laimonas Kelbauskas , Honor Glenn , Jeff Houkal , Clifford Anderson , Yanqing Tian , Fengyu Su , Deirdre Meldrum
Abstract: A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation is provided. In certain aspects, an integrated microfluidic device for multi-parameter metabolic and other phenotypic profiling of live biological cells is useable with: 1) multicellular clusters or small biopsy tissue samples, 2) cultures of the constituent cells obtained after cluster/tissue dissociation, and 3) the same constituent single cells in isolation. The approach enables study of the effects of multicellular complexity, such as in response to treatment, pathogens, stress, or other factors concerning disease origination and progression. Measurements may be performed on single cells or multicellular populations or tissues in the same assay at the same time.
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公开(公告)号：US11136614B2
公开(公告)日：2021-10-05
申请号：US15757712
申请日：2016-10-07
Inventor： Jacob Messner , Clifford Anderson , Honor Glenn , Kristen Lee , Mark Richards , Laimonas Kelbauskas , Kimberly Bussey , Deirdre Meldrum
IPC: C12Q1/24 , C12M1/00 , C12M1/32 , G01N33/569
Abstract: Methods for seeding live cells onto spatially defined regions of a substrate including multiple features (e.g., microwells or other microenvironments) utilize a stencil embodied in a hole-defining sacrificial film. A sacrificial film devoid of holes may be applied over features of a substrate, and a hole generating mechanism (e.g., hot needle or laser) aligned with features may be used to define holes in the film. Alternatively, holes may be predefined in a sacrificial film to form a stencil, and the stencil may be assembled to the substrate with the holes registered with features thereof. Thereafter, cells are seeded through holes in the film. Seeded cells are subject to incubation, further processing, and/or performance of one or more assays, and the hole-defining sacrificial film (stencil) may be removed.
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公开(公告)号：US10940476B2
公开(公告)日：2021-03-09
申请号：US16095375
申请日：2017-04-21
Inventor： Laimonas Kelbauskas , Honor Glenn , Jeff Houkal , Clifford Anderson , Yanqing Tian , Fengyu Su , Deirdre Meldrum
Abstract: A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation is provided. In certain aspects, an integrated microfluidic device for multi-parameter metabolic and other phenotypic profiling of live biological cells is useable with: 1) multicellular clusters or small biopsy tissue samples, 2) cultures of the constituent cells obtained after cluster/tissue dissociation, and 3) the same constituent single cells in isolation. The approach enables study of the effects of multicellular complexity, such as in response to treatment, pathogens, stress, or other factors concerning disease origination and progression. Measurements may be performed on single cells or multicellular populations or tissues in the same assay at the same time.
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公开(公告)号：US20180334700A1
公开(公告)日：2018-11-22
申请号：US15757712
申请日：2016-10-07
Inventor： Jacob Messner , Clifford Anderson , Honor Glenn , Kristen Lee , Mark Richards , Laimonas Kelbauskas , Kimberly Bussey , Deirdre Meldrum
IPC: C12Q1/24 , G01N33/569
CPC classification number: C12Q1/24 , C12M1/00 , C12M23/12 , G01N33/56966
Abstract: Methods for seeding live cells onto spatially defined regions of a substrate including multiple features (e.g., microwells or other microenvironments) utilize a stencil embodied in a hole-defining sacrificial film. A sacrificial film devoid of holes may be applied over features of a substrate, and a hole generating mechanism (e.g., hot needle or laser) aligned with features may be used to define holes in the film. Alternatively, holes may be predefined in a sacrificial film to form a stencil, and the stencil may be assembled to the substrate with the holes registered with features thereof. Thereafter, cells are seeded through holes in the film. Seeded cells are subject to incubation, further processing, and/or performance of one or more assays, and the hole-defining sacrificial film (stencil) may be removed.
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