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公开(公告)号：US20180318835A1
公开(公告)日：2018-11-08
申请号：US16035464
申请日：2018-07-13
Inventor： Rhett Martineau , Jeff Houkal , Shih-Hui Chao , Weimin Gao , Shufang Ci , Deirdre Meldrum
CPC classification number: B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L7/52 , B01L2200/0605 , B01L2200/0642 , B01L2200/0647 , B01L2200/0684 , B01L2300/044 , B01L2300/0636 , B01L2300/0681 , B01L2300/0803 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/0883 , B01L2300/161 , B01L2400/049 , B01L2400/0683
Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.
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公开(公告)号：US11315292B2
公开(公告)日：2022-04-26
申请号：US16487535
申请日：2018-03-02
Inventor： Deirdre Meldrum , Roger Johnson , Laimonas Kelbauskas , Jeff Houkal , Brian Ashcroft , Dean Smith , Hong Wang , Shih-Hui (Joseph) Chao , Rishabh Shetty , Jakrey Myers , Iniyan Soundappa Elango
IPC: G06T11/00 , G06T7/194 , G06T7/30 , G01N15/02 , G01N15/14 , G06T5/00 , G06T5/50 , G06T17/00 , G01N15/10
Abstract: Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-in-volves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.
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公开(公告)号：US10940476B2
公开(公告)日：2021-03-09
申请号：US16095375
申请日：2017-04-21
Inventor： Laimonas Kelbauskas , Honor Glenn , Jeff Houkal , Clifford Anderson , Yanqing Tian , Fengyu Su , Deirdre Meldrum
Abstract: A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation is provided. In certain aspects, an integrated microfluidic device for multi-parameter metabolic and other phenotypic profiling of live biological cells is useable with: 1) multicellular clusters or small biopsy tissue samples, 2) cultures of the constituent cells obtained after cluster/tissue dissociation, and 3) the same constituent single cells in isolation. The approach enables study of the effects of multicellular complexity, such as in response to treatment, pathogens, stress, or other factors concerning disease origination and progression. Measurements may be performed on single cells or multicellular populations or tissues in the same assay at the same time.
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公开(公告)号：US20160237476A1
公开(公告)日：2016-08-18
申请号：US15135150
申请日：2016-04-21
Inventor： Deirdre Meldrum , Shih-Hui (Joseph) Chao , Thai Tran , Laimonas Kelbauskas , Jeff Houkal , Andrew Hatch , Weimin Gao , David Richardson
IPC: C12Q1/68
CPC classification number: C12Q1/6806 , B01L3/0293 , B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , C12M47/06 , C12Q1/6841 , C12Q1/6848 , C12Q1/686 , G01N1/286 , G01N2001/2886 , C12Q2547/101
Abstract: Systems and methods for in situ laser lysis for analysis of biological tissue (live, fixed, frozen or otherwise preserved) at single cell resolution in 3D. For example, a system and method for lysing individual cells in situ, including the steps of capturing a tissue sample comprising a cellular content, subjecting the tissue sample to a stream of continuous fluid flow, lysing a selected area of the tissue sample with a laser, thereby releasing at least a portion of the cellular content from the tissue sample, recovering at least one target molecule from the cellular content in the stream, and processing at least one target molecule is provided. The system collects cellular contents, performs highly multiplexed (RT-qPCR or RNA-seq), and sequentially (cell-by-cell) reconstructs a 3D spatial map of mRNA expression of the tissue with a large number of genes. A 3D spatial map of the DNA, RNA, and/or proteins can be generated for each cell in the tissue.
Abstract translation: 用于原位激光裂解的系统和方法，用于分析3D中单细胞分辨率的生物组织（活，固定，冷冻或以其他方式保存）。 例如，用于原位裂解单个细胞的系统和方法，包括以下步骤：捕获包含细胞内容物的组织样品，使组织样品经受连续流体流的流动，用激光裂解组织样品的选定区域 从而从组织样品释放至少一部分细胞含量，从流中的细胞含量回收至少一种靶分子，并且提供至少一种靶分子的加工。 系统收集细胞内容，进行高度多重化（RT-qPCR或RNA-seq），并依次（逐个细胞）重构具有大量基因的组织的mRNA表达的3D空间图。 可以为组织中的每个细胞产生DNA，RNA和/或蛋白质的3D空间图。
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公开(公告)号：US10221443B2
公开(公告)日：2019-03-05
申请号：US15135150
申请日：2016-04-21
Inventor： Deirdre Meldrum , Shih-Hui (Joseph) Chao , Thai Tran , Laimonas Kelbauskas , Jeff Houkal , Andrew Hatch , Weimin Gao , David Richardson
IPC: C12Q1/68 , C12Q1/6806 , C12M1/00 , G01N1/28 , C12Q1/6848 , B01L3/02 , B01L3/00 , C12Q1/6841 , C12Q1/686
Abstract: Systems and methods for in situ laser lysis for analysis of biological tissue (live, fixed, frozen or otherwise preserved) at single cell resolution in 3D. For example, a system and method for lysing individual cells in situ, including the steps of capturing a tissue sample comprising a cellular content, subjecting the tissue sample to a stream of continuous fluid flow, lysing a selected area of the tissue sample with a laser, thereby releasing at least a portion of the cellular content from the tissue sample, recovering at least one target molecule from the cellular content in the stream, and processing at least one target molecule is provided. The system collects cellular contents, performs highly multiplexed (RT-qPCR or RNA-seq), and sequentially (cell-by-cell) reconstructs a 3D spatial map of mRNA expression of the tissue with a large number of genes. A 3D spatial map of the DNA, RNA, and/or proteins can be generated for each cell in the tissue.
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Patent Agency Ranking
公开(公告)号：US10022718B2
公开(公告)日：2018-07-17
申请号：US14922696
申请日：2015-10-26
Inventor： Rhett Martineau , Jeff Houkal , Shih-Hui Chao , Weimin Gao , Shufang Ci , Deirdre Meldrum
Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.
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公开(公告)号：US11327004B2
公开(公告)日：2022-05-10
申请号：US16082170
申请日：2017-03-02
Inventor： Deirdre Meldrum , Roger Johnson , Laimonas Kelbauskas , Jeff Houkal , Brian Ashcroft , Dean Smith , Hong Wang , Shih-Hui Joseph Chao , Rishabh Shetty , Jakrey Myers , Iniyan Soundappa Elango
Abstract: Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-involves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.
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公开(公告)号：US20200058140A1
公开(公告)日：2020-02-20
申请号：US16487535
申请日：2018-03-02
Inventor： Deirdre Meldrum , Roger Johnson , Laimonas Kelbauskas , Jeff Houkal , Brian Ashcroft , Dean Smith , Hong Wang , Shih-Hui (Joseph) Chao , Rishabh Shetty , Jakrey Myers , Iniyan Soundappa Elango
Abstract: Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-in-volves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.
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公开(公告)号：US10471426B2
公开(公告)日：2019-11-12
申请号：US16035464
申请日：2018-07-13
Inventor： Rhett Martineau , Jeff Houkal , Shih-Hui Chao , Weimin Gao , Shufang Ci , Deirdre Meldrum
Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.
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10.发明申请公开(公告)号：US20190126275A1
公开(公告)日：2019-05-02
申请号：US16095375
申请日：2017-04-21
Inventor： Laimonas Kelbauskas , Honor Glenn , Jeff Houkal , Clifford Anderson , Yanqing Tian , Fengyu Su , Deirdre Meldrum
Abstract: A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation is provided. In certain aspects, an integrated microfluidic device for multi-parameter metabolic and other phenotypic profiling of live biological cells is useable with: 1) multicellular clusters or small biopsy tissue samples, 2) cultures of the constituent cells obtained after cluster/tissue dissociation, and 3) the same constituent single cells in isolation. The approach enables study of the effects of multicellular complexity, such as in response to treatment, pathogens, stress, or other factors concerning disease origination and progression. Measurements may be performed on single cells or multicellular populations or tissues in the same assay at the same time.
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