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公开(公告)号：US11156619B2
公开(公告)日：2021-10-26
申请号：US16479373
申请日：2018-01-19
Inventor： Liqiang Zhang , Xiangxing Kong , Yanqing Tian , Deirdre Meldrum , Fengyu Su
IPC: G01N21/64 , G01N33/84 , C07D471/16 , C08F220/58 , C12Q1/02 , G01N31/22 , G01N33/50 , G01N21/77
Abstract: Fluorescent pH sensors are provided. The fluorescent pH sensor comprises a copolymer for sensing pH and a polymerized form of N-(2-hydroxypropyl)methacrylamide (HPMA) or 2-hydroxyethyl methacrylate (HEMA). The probe for sensing pH has formula (I): wherein R1 and R2 are as defined herein. The fluorescent pH sensors may be used in determining the pH of a sample and detecting extracellular pH in a sample. Methods for preparing the fluorescent pH sensors and the probe for sensing pH are also provided.
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公开(公告)号：US10590155B2
公开(公告)日：2020-03-17
申请号：US16314753
申请日：2017-07-14
Inventor： Xiangxing Kong , Yanqing Tian , Fengyu Su , Liqiang Zhang , Deirdre Meldrum
IPC: C07F9/6596 , G01N33/50 , G01N33/58 , G01N33/68
Abstract: Mitochondria-targeting potassium sensors and method(s) for making such sensors. The sensor shows a response to potassium and displays a 130-fold dynamic range of fluorescence intensity and high brightness. The sensors response to potassium concentrations was demonstrated to be unaffected by cellular pH value and/or concentrations of other ions. The sensors can be used for monitoring the mitochondrial potassium efflux/influx.
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3.发明申请公开(公告)号：US20190126275A1
公开(公告)日：2019-05-02
申请号：US16095375
申请日：2017-04-21
Inventor： Laimonas Kelbauskas , Honor Glenn , Jeff Houkal , Clifford Anderson , Yanqing Tian , Fengyu Su , Deirdre Meldrum
Abstract: A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation is provided. In certain aspects, an integrated microfluidic device for multi-parameter metabolic and other phenotypic profiling of live biological cells is useable with: 1) multicellular clusters or small biopsy tissue samples, 2) cultures of the constituent cells obtained after cluster/tissue dissociation, and 3) the same constituent single cells in isolation. The approach enables study of the effects of multicellular complexity, such as in response to treatment, pathogens, stress, or other factors concerning disease origination and progression. Measurements may be performed on single cells or multicellular populations or tissues in the same assay at the same time.
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公开(公告)号：US11364502B2
公开(公告)日：2022-06-21
申请号：US15774558
申请日：2016-11-14
Inventor： Deirdre Meldrum , Laimonas Kelbauskas , Wacey Teller , Meryl Rodrigues , Hong Wang , Ganquan Song , Yanqing Tian , Fengyu Su , Xiangxing Kong , Liqiang Zhang
Abstract: A microfluidic device includes a first substrate including at least one microfluidic channel and a plurality of microwells, as well as a cooperating second substrate defining multiple split-walled cell trap structures that are registered with and disposed within the plurality of microwells. A method for performing an assay includes flowing cells and a first aqueous medium into a plurality of microwells of a microfluidic device, wherein each microwell includes a cell trap structure configured to trap at least one cell. The method further comprises flowing a nonpolar fluid with low permeability for analytes of interest through a microfluidic channel to flush a portion of the first aqueous medium from the microfluidic channel while retaining another portion of the first aqueous medium and at least one cell within each microwell. Surface tension at a non-polar/polar medium interface prevents molecule exchange between interior and exterior portions of microwells.
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公开(公告)号：US10940476B2
公开(公告)日：2021-03-09
申请号：US16095375
申请日：2017-04-21
Inventor： Laimonas Kelbauskas , Honor Glenn , Jeff Houkal , Clifford Anderson , Yanqing Tian , Fengyu Su , Deirdre Meldrum
Abstract: A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation is provided. In certain aspects, an integrated microfluidic device for multi-parameter metabolic and other phenotypic profiling of live biological cells is useable with: 1) multicellular clusters or small biopsy tissue samples, 2) cultures of the constituent cells obtained after cluster/tissue dissociation, and 3) the same constituent single cells in isolation. The approach enables study of the effects of multicellular complexity, such as in response to treatment, pathogens, stress, or other factors concerning disease origination and progression. Measurements may be performed on single cells or multicellular populations or tissues in the same assay at the same time.
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Patent Agency Ranking
6.发明申请公开(公告)号：US20200047182A1
公开(公告)日：2020-02-13
申请号：US15774558
申请日：2016-11-14
Inventor： Deirdre Meldrum , Laimonas Kelbauskas , Wacey Teller , Meryl Rodrigues , Hong Wang , Ganquan Song , Yanqing Tian , Fengyu Su , Xiangxing Kong , Liqiang Zhang
IPC: B01L3/00
Abstract: A microfluidic device includes a first substrate including at least one microfluidic channel and a plurality of microwells, as well as a cooperating second substrate defining multiple split-walled cell trap structures that are registered with and disposed within the plurality of microwells. A method for performing an assay includes flowing cells and a first aqueous medium into a plurality of microwells of a microfluidic device, wherein each microwell includes a cell trap structure configured to trap at least one cell. The method further comprises flowing a nonpolar fluid with low permeability for analytes of interest through a microfluidic channel to flush a portion of the first aqueous medium from the microfluidic channel while retaining another portion of the first aqueous medium and at least one cell within each microwell. Surface tension at a non-polar/polar medium interface prevents molecule exchange between interior and exterior portions of microwells.
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公开(公告)号：US20190153005A1
公开(公告)日：2019-05-23
申请号：US16314753
申请日：2017-07-14
Inventor： Xiangxing Kong , Yanqing Tian , Fengyu Su , Liqiang Zhang , Deirdre Meldrum
IPC: C07F9/6596 , G01N33/50 , G01N33/58 , G01N33/68
CPC classification number: C07F9/6596 , A61K31/69 , C07D498/02 , C07D498/08 , C07D498/18 , G01N33/5079 , G01N33/582 , G01N33/6872
Abstract: Mitochondria-targeting potassium sensors and method(s) for making such sensors. The sensor shows a response to potassium and displays a 130-fold dynamic range of fluorescence intensity and high brightness. The sensors response to potassium concentrations was demonstrated to be unaffected by cellular pH value and/or concentrations of other ions. The sensors can be used for monitoring the mitochondrial potassium efflux/influx.
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公开(公告)号：US20200178967A1
公开(公告)日：2020-06-11
申请号：US16631040
申请日：2018-08-29
Inventor： Deirdre Meldrum , Fengyu Su , Laimonas Kelbauskas , Vivek Nandakumar , Yanqing Tian , Roger Johnson
Abstract: A thixotropic and biocompatible gel, formed by combining of 4-arm polyethylene glycol with fumed silica (PEG-silica). The thixotropicity of the gel is affected by the ratio of PEG to silica. To study the gels biocompatibility, the cell suspension in PBS or another medium was added to the PEG-silica gel via mixing to uniformly disperse the cells in the gel and then placed in an incubator before performing fluorescence live-dead assays. Duration of cell viability (cell life time) in gels was measured to be up to several days, depending on gel composition. Due to their optical and fluidic properties the gels are compatible with live cell imaging including 3D computed tomography and offer a means for moving the cells in a highly controllable manner by applying and removing pressure on the gels.
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公开(公告)号：US11369966B2
公开(公告)日：2022-06-28
申请号：US15760984
申请日：2016-09-16
Inventor： Clifford Anderson , Kristen Lee , Jacob Messner , Laimonas Kelbauskas , Benjamin Ueberroth , Yanqing Tian , Deirdre Meldrum
IPC: B01L3/00
Abstract: A multi-layer sealing structure for sealing a microwell array defined in or on a substrate includes at least one front compliant layer, a back compliant layer, and a flexural layer arranged between the at least one front compliant layer and the back compliant layer, wherein the at least one front compliant layer is closer than the back compliant layer to microwells of the microwell array. One or more front compliant layers may be optically reflective and/or may embody a sensor layer. The back compliant layer may include an adhesive or various types of rubber, and the flexural layer may include a polymeric material or metal. A multi-layer sealing structure may be separated from a microwell array by peeling. A multi-layer sealing structure allows local disruption of sealing where particle contaminants are present without compromising the sealing performance of an entire microwell array, and without requiring a large sealing force.
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公开(公告)号：US20180264468A1
公开(公告)日：2018-09-20
申请号：US15760984
申请日：2016-09-16
Inventor： Clifford Anderson , Kristen Lee , Jacob Messner , Laimonas Kelbauskas , Benjamin Ueberroth , Yanqing Tian , Deirdre Meldrum
IPC: B01L3/00
CPC classification number: B01L3/50853 , B01L2200/025 , B01L2200/0689 , B01L2200/141 , B01L2200/142 , B01L2300/0627 , B01L2300/0829 , B01L2300/123 , B01L2300/16
Abstract: A multi-layer sealing structure for sealing a microwell array defined in or on a substrate includes at least one front compliant layer, a back compliant layer, and a flexural layer arranged between the at least one front compliant layer and the back compliant layer, wherein the at least one front compliant layer is closer than the back compliant layer to microwells of the microwell array. One or more front compliant layers may be optically reflective and/or may embody a sensor layer. The back compliant layer may include an adhesive or various types of rubber, and the flexural layer may include a polymeric material or metal. A multi-layer sealing structure may be separated from a microwell array by peeling. A multi-layer sealing structure allows local disruption of sealing where particle contaminants are present without compromising the sealing performance of an entire microwell array, and without requiring a large sealing force.
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