-
公开(公告)号：US11327004B2
公开(公告)日：2022-05-10
申请号：US16082170
申请日：2017-03-02
Inventor： Deirdre Meldrum , Roger Johnson , Laimonas Kelbauskas , Jeff Houkal , Brian Ashcroft , Dean Smith , Hong Wang , Shih-Hui Joseph Chao , Rishabh Shetty , Jakrey Myers , Iniyan Soundappa Elango
Abstract: Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-involves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.
-
公开(公告)号：US20200058140A1
公开(公告)日：2020-02-20
申请号：US16487535
申请日：2018-03-02
Inventor： Deirdre Meldrum , Roger Johnson , Laimonas Kelbauskas , Jeff Houkal , Brian Ashcroft , Dean Smith , Hong Wang , Shih-Hui (Joseph) Chao , Rishabh Shetty , Jakrey Myers , Iniyan Soundappa Elango
Abstract: Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-in-volves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.
-
公开(公告)号：US20200178967A1
公开(公告)日：2020-06-11
申请号：US16631040
申请日：2018-08-29
Inventor： Deirdre Meldrum , Fengyu Su , Laimonas Kelbauskas , Vivek Nandakumar , Yanqing Tian , Roger Johnson
Abstract: A thixotropic and biocompatible gel, formed by combining of 4-arm polyethylene glycol with fumed silica (PEG-silica). The thixotropicity of the gel is affected by the ratio of PEG to silica. To study the gels biocompatibility, the cell suspension in PBS or another medium was added to the PEG-silica gel via mixing to uniformly disperse the cells in the gel and then placed in an incubator before performing fluorescence live-dead assays. Duration of cell viability (cell life time) in gels was measured to be up to several days, depending on gel composition. Due to their optical and fluidic properties the gels are compatible with live cell imaging including 3D computed tomography and offer a means for moving the cells in a highly controllable manner by applying and removing pressure on the gels.
-
公开(公告)号：US11315292B2
公开(公告)日：2022-04-26
申请号：US16487535
申请日：2018-03-02
Inventor： Deirdre Meldrum , Roger Johnson , Laimonas Kelbauskas , Jeff Houkal , Brian Ashcroft , Dean Smith , Hong Wang , Shih-Hui (Joseph) Chao , Rishabh Shetty , Jakrey Myers , Iniyan Soundappa Elango
IPC: G06T11/00 , G06T7/194 , G06T7/30 , G01N15/02 , G01N15/14 , G06T5/00 , G06T5/50 , G06T17/00 , G01N15/10
Abstract: Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-in-volves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.
-
-
-
Search Results
4
records
(took 0.02 seconds)
