公开(公告)号：US4508828A

公开(公告)日：1985-04-02

申请号：US477253

申请日：1983-03-21

申请人： Arnold W. Lindall ,  James J. Elting

发明人： Arnold W. Lindall ,  James J. Elting

IPC分类号： G01N33/78 ,  G01N33/56 ,  G01N33/58 ,  G01N33/60

CPC分类号： G01N33/78 ,  Y10S436/804 ,  Y10S436/808 ,  Y10S436/817 ,  Y10S436/824 ,  Y10S436/825

摘要： A method is disclosed for extraction, concentration, and bioassay measurement of the concentration of bioactive parathyroid peptides in biological or other fluids. Parathyroid peptides are extracted from the fluid by a support-antibody matrix specific to the N-terminal region. The bioassay is directed to the extracted parathyroid peptides. The volume of the medium containing the extracted parathyroid peptides and tissue, cells or tissue extracts with adenylate cyclase-coupled parathyroid hormone receptors may be chosen to be significantly less than the volume of biological or other fluid being assayed.

摘要翻译： 公开了用于提取，浓缩和生物测定生物或其它流体中生物活性甲状旁腺肽浓度的方法。 通过特异于N-末端区域的载体 - 抗体基质从流体中提取甲状旁腺肽。 生物测定指向提取的甲状旁腺肽。 含有提取的甲状旁腺肽和组织，含腺苷酸环化酶偶联甲状旁腺激素受体的细胞或组织提取物的培养基的体积可以选择为显着小于被测定的生物或其他流体的体积。

公开(公告)号：US4341755A

公开(公告)日：1982-07-27

申请号：US169492

申请日：1980-07-15

申请人： Arnold W. Lindall

发明人： Arnold W. Lindall

IPC分类号： G01N33/53 ,  G01N33/78 ,  G01N33/56

CPC分类号： G01N33/78

摘要： A method for the radioimmunoassay of parathyroid hormone in mammalian serum. The method is an improvement on previous double antibody radioimmunoassays in that spurious assay results caused by the nonspecific interaction of serum proteins with the labeled peptide is eliminated by the labeling of a specific portion of either human or bovine parathyroid molecule. The 65-84 portion of human or bovine parathyroid hormone, as radioactively labeled, is incorporated in the assay as the labeled peptide. A chicken antibody with a high affinity for the 65-84 portion of human parathyroid hormone is incorporated in the assay as the first antibody. The invention further pertains to compounds useful in practicing the method, namely X.sup.64 -hPTH.sup.65-84 and Y-X.sup.64 -hPTH.sup.65-84 wherein X is either histidyl, tyramyl, histamyl, or tyrosyl, and wherein Y is either .sup.125 I or .sup.131 I.

摘要翻译： 哺乳动物血清中甲状旁腺激素放射免疫测定的方法。 该方法是对先前的双抗体放射免疫测定的改进，因为通过标记人或牛甲状旁腺分子的特定部分来消除由血清蛋白与标记肽的非特异性相互作用引起的杂散测定结果。 作为放射性标记的人或牛甲状旁腺激素的65-84部分作为标记肽掺入测定中。 将作为人甲状旁腺激素65-84部分的高亲和力的鸡抗体作为第一抗体并入测定中。 本发明还涉及可用于实施该方法的化合物，即X64-hPTH65-84和Y-X64-hPTH65-84，其中X为组氨酰，酪氨酰，组氨酰或酪氨酰，其中Y为125 I或131 I。

公开(公告)号：US5470307A

公开(公告)日：1995-11-28

申请号：US214051

申请日：1994-03-16

申请人： Arnold W. Lindall

发明人： Arnold W. Lindall

IPC分类号： A61L29/14 ,  A61L29/16 ,  A61M25/00 ,  A61N5/06 ,  A61M1/00

CPC分类号： A61N5/062 ,  A61L29/14 ,  A61L29/16 ,  A61N5/0601 ,  A61L2300/22 ,  A61L2300/222 ,  A61L2300/23 ,  A61L2300/25 ,  A61L2300/252 ,  A61L2300/258 ,  A61L2300/602 ,  A61M2025/0057

摘要： A catheter having a therapeutic agent chemically bonded to a substrate on its exterior surface using a linker which photolytically releases the agent upon exposure to light energy at an appropriate wavelength. The linker is attached to the substrate via a complementary chemical group, with the opposing end containing an aromatic ring with a nitro group in the ortho position relative to a methyl or ethyl group which is functionalized to accept a complementary bond to the therapeutic agent. The substrate may include materials such as glass, polyamide, polyester, polyolefin, polypropylene, polyurethane, or latex. The therapeutic agent may include peptides, proteins, steroids, carbohydrates, nucleotides, or other aliphatic or heterocyclic products, and may be bonded to a molecular lattice or meshwork to accommodate a high molecular concentration per unit area and the inclusion of ancillary compounds such as markers or secondary emitters.

摘要翻译： 一种具有治疗剂的导管，其使用在暴露于适当波长的光能下光解释放试剂的接头与其外表面上的基底化学键合。 接头通过互补的化学基团连接到基底上，相对端含有相对于被官能化以接受与治疗剂的互补键的甲基或乙基的邻位的硝基的芳环。 基材可以包括诸如玻璃，聚酰胺，聚酯，聚烯烃，聚丙烯，聚氨酯或胶乳的材料。 治疗剂可以包括肽，蛋白质，类固醇，碳水化合物，核苷酸或其它脂族或杂环产物，并且可以结合到分子晶格或网状物以适应每单位面积的高分子浓度，并且包含辅助化合物如标记 或二次发射器。

公开(公告)号：US4369138A

公开(公告)日：1983-01-18

申请号：US302734

申请日：1981-09-16

申请人： Arnold W. Lindall

发明人： Arnold W. Lindall

IPC分类号： G01N33/78 ,  C07C103/52

CPC分类号： G01N33/78 ,  Y10S530/854

摘要： A method for the radioimmunoassay of parathyroid hormone in mammalian serum. The method is an improvement on previous double antibody radioimmunoassays in that spurious assay results caused by the nonspecific interaction of serum proteins with the labeled peptide is eliminated by the labeling of a specific portion of either human or bovine parathyroid molecule. The 65-84 portion of human or bovine parathyroid hormone, as radioactively labeled, is incorporated in the assay as the labeled peptide. A chicken antibody with a high affinity for the 65-84 portion of human parathyroid hormone is incorporated in the assay as the first antibody. The invention further pertains to compounds useful in practicing the method, namely X.sup.64 -hPTH.sup.65-84 and Y-X.sup.64 -hPTH.sup.65-84 wherein X is either histidyl, tyramyl, histamyl, or tyrosyl, and wherein Y is either .sup.125 I or .sup.131 I.