公开(公告)号：US07282351B2

公开(公告)日：2007-10-16

申请号：US10270377

申请日：2002-10-15

Applicant: Peter L. Hudson ,  Craig A. Rosen ,  Wei-Wu He

Inventor： Peter L. Hudson ,  Craig A. Rosen ,  Wei-Wu He

IPC: C07H21/04 ,  C12N5/10 ,  C12N15/63 ,  C12P21/02

CPC classification number: C07K16/22 ,  A61K38/00 ,  A61K39/00 ,  C07K14/475 ,  C07K14/575 ,  Y10S530/854

Abstract: The present invention relates to human PGF polypeptides and DNA (RNA) encoding such polypeptides. Also provided is a procedure for producing such polypeptides by recombinant techniques, and antibodies and antagonist/inhibitors against such polypeptides. Also provided are methods of using such polypeptides therapeutically for treating prostate cancer, to promote tissue regeneration and to facilitate wound healing. Also provided is a diagnostic assay to detect prostate cancer and benign prostatic hyperplasia.

Abstract translation: 本发明涉及人PGF多肽和编码这种多肽的DNA（RNA）。 还提供了通过重组技术产生这种多肽的方法，以及针对这种多肽的抗体和拮抗剂/抑制剂。 还提供了使用这些多肽治疗性治疗前列腺癌，促进组织再生和促进伤口愈合的方法。 还提供了用于检测前列腺癌和良性前列腺增生的诊断测定法。

公开(公告)号：US06537760B1

公开(公告)日：2003-03-25

申请号：US09319570

申请日：1999-08-04

Applicant: Andreas Bergmann ,  Joachim Struck

Inventor： Andreas Bergmann ,  Joachim Struck

IPC: G01N3353

CPC classification number: G01N33/76 ,  G01N33/686 ,  Y10S435/963 ,  Y10S435/965 ,  Y10S435/975 ,  Y10S436/811 ,  Y10S530/854

Abstract: In a competitive receptor binding assay for detecting TSH-receptor auto-antibodies in a biological sample, the sample is reacted in a reaction mixture which contains (i) a TSH-receptor or TSH-receptor preparation; (ii) a primary competitor, for example labelled TSH; and (iii) an agent for separating a complex composed of the TSH-receptor and the elements bound thereto of the reaction mixture from the liquid phase. According to the invention, the reaction is carried out in the presence of at least one monoclonal or polyclonal antibody specific against a partial peptide sequence of the TSH eceptor. This specific antibody is used to immobilize a complex of TSH-receptor and primary competitor and/or as secondary competitor for another part of the TSH-receptor auto-antibodies expected in a sample. The primary or secondary competitors are or can be selectively labelled.

Abstract translation: 在用于检测生物样品中TSH-受体自身抗体的竞争性受体结合测定中，样品在含有（i）TSH受体或TSH-受体制剂的反应混合物中反应; （ii）主要竞争对手，例如标签为TSH; 和（iii）用于从液相中分离由TSH-受体组成的络合物和与反应混合物结合的元素的试剂。 根据本发明，反应在至少一种针对TSH受体的部分肽序列特异性的单克隆或多克隆抗体的存在下进行。 该特异性抗体用于固定TSH受体和主要竞争物的复合物和/或作为样品中预期的另一部分TSH-受体自身抗体的次要竞争物。 主要或次要竞争对手是或可以选择性标签。

公开(公告)号：US5998153A

公开(公告)日：1999-12-07

申请号：US313200

申请日：1994-11-08

Applicant: James R. Baker, Jr. ,  Ronald J. Koenig

Inventor： James R. Baker, Jr. ,  Ronald J. Koenig

IPC: A61K38/00 ,  A61K39/00 ,  C12N9/08 ,  G01N33/564 ,  G01N33/573 ,  A61K38/43

CPC classification number: C12N9/0065 ,  G01N33/564 ,  G01N33/573 ,  A61K38/00 ,  A61K39/00 ,  G01N2800/24 ,  Y10S435/975 ,  Y10S436/811 ,  Y10S530/854

Abstract: Specific epitopic regions of thyroid peroxidase (TPO), a thyroid specific membrane autoantigen, have been identified within amino acid residues 456 to 933, 517 to 630, and 633 to 933 of the protein (SEQ ID NO:1), with at least one distinct binding region within TPO located from amino acid residues 592 to 613 (SEQ ID NO:3). The identification and production of these localized epitopes/epitopic regions provide specific diagnostic reagents for autoimmune thyroid disease.

Abstract translation: PCT No.PCT / US93 / 03837 Sec。 371日期：1994年11月8日 102（e）日期1994年11月8日PCT提交1993年4月22日PCT公布。 公开号WO93 / 23073 日期1997年11月25日已经在蛋白质（SEQ ID NO：1）的456至933,517至630和633至933的氨基酸残基内鉴定了甲状腺过氧化物酶（TPO），甲状腺特异性膜自身抗原的特异性表位区域， ，在TPO内至少有一个与氨基酸残基592至613（SEQ ID NO：3）不同的结合区。 鉴定和产生这些局部表位/表位区为自身免疫性甲状腺疾病提供了特异性的诊断试剂。

公开(公告)号：US5994102A

公开(公告)日：1999-11-30

申请号：US411607

申请日：1995-10-02

Applicant: Peter L. Hudson ,  Craig A. Rosen ,  Wei Wu He

Inventor： Peter L. Hudson ,  Craig A. Rosen ,  Wei Wu He

IPC: A61K39/00 ,  C07K14/575 ,  C07K16/22 ,  C12N1/21 ,  C12N15/12 ,  C07K14/475 ,  C12N15/63

CPC classification number: C07K16/22 ,  C07K14/475 ,  C07K14/575 ,  A61K38/00 ,  A61K39/00 ,  Y10S530/854

Abstract: The present invention relates to polynucleotides encoding human PGF polypeptides, variant polynucleotides encoding analog polypeptides, and variant polynucleotides useful as probes for polynucleotides encoding human PGF polypeptides. Also provided is a procedure for producing such polypeptides by recombinant techniques.

Abstract translation: PCT No.PCT / US94 / 14578 Sec。 371 1995年10月2日日期 102（e）日期1995年10月2日PCT 1994年12月15日PCT PCT。 出版物WO96 / 日期：1996年6月20日本发明涉及编码人PGF多肽的多核苷酸，编码类似物多肽的变体多核苷酸和用作编码人PGF多肽的多核苷酸的探针的变体多核苷酸。 还提供了通过重组技术产生此类多肽的方法。

公开(公告)号：US5449766A

公开(公告)日：1995-09-12

申请号：US208531

申请日：1994-03-09

Applicant: Joan Vaughan ,  Wolfgang H. Fischer ,  Jean E. F. Rivier ,  Jean-Louis M. Nahon ,  Francoise G. Presse ,  Wylie W. Vale, Jr.

Inventor： Joan Vaughan ,  Wolfgang H. Fischer ,  Jean E. F. Rivier ,  Jean-Louis M. Nahon ,  Francoise G. Presse ,  Wylie W. Vale, Jr.

IPC: C12N15/09 ,  A61K38/00 ,  A61K38/04 ,  A61P35/00 ,  C07K7/06 ,  C07K7/08 ,  C07K7/64 ,  C07K14/00 ,  C07K14/575 ,  C12N15/12 ,  C12N15/16 ,  C07H12/00 ,  C07H21/00 ,  C07K1/00 ,  C12P21/06

CPC classification number: C07K7/08 ,  C07K7/64 ,  A61K38/00 ,  Y10S530/827 ,  Y10S530/854

Abstract: Mammalian melanin-concentrating hormone (MCH) is isolated from rat tissue, purified and characterized. These MCH peptides are useful for treating skin disorders, for suppressing the proliferation of skin tumor cells, such as melanomas in mammals, and for modulating the secretion of ACTH. Generally, peptides are provided which have the following formula: ##STR1## or which are naturally occurring homologs of the peptide with said formula. The peptides which are the naturally occurring MCH homologs of mammalian species other than rat can also be obtained using the materials disclosed, as demonstrated specifically with human MCH, which is found to have the same structure as rat MCH. Also disclosed are the amino acid sequences of, and the nucleotide sequences of the cDNAs which encode, the putative precursors of rat MCH and human MCH. These precursors may also include one or more biologically active peptides N-terminally of the mature MCH's. Among these peptides, which are thought to be formed from the MCH precursors, are the peptide with the sequence H-Glu-Ile-Gly-Asp-Glu-Glu-Asn-Ser-Ala-Lys-Phe-Pro-Ile-NH.sub.2, which is cross-reactive with antibodies against alpha-MSH and CRF, and the peptide with the sequence H-Gly-X.sub.NGE -Phe-Pro-Ala-Glu-Asn-Gly-Val-Gln-Asn-Thr-Glu-Ser-Thr-Gln-Glu-OH, wherein X.sub.NGE is Pro-Ala-Val or Ser-Val-Ala, which is cross-reactive with antibodies against GRF.

Abstract translation: 从大鼠组织中分离哺乳动物黑色素浓缩激素（MCH），进行纯化和表征。 这些MCH肽可用于治疗皮肤病症，抑制皮肤肿瘤细胞增殖，如哺乳动物的黑素瘤，以及调节ACTH的分泌。 通常，提供具有下式的肽：＆lt; IMAGE＆gt;或具有所述式的肽的天然存在同系物。 作为除大鼠之外的哺乳动物物种的天然存在的MCH同源物的肽也可以使用公开的材料获得，如用人MCH特异性证实的，其被发现具有与大鼠MCH相同的结构。 还公开了编码大鼠MCH和人MCH的推定前体的cDNA的氨基酸序列和cDNA的核苷酸序列。 这些前体还可以包括成熟MCH的N-末端的一种或多种生物活性肽。 在这些被认为由MCH前体形成的肽中，具有序列H-Glu-Ile-Gly-Asp-Glu-Glu-Asn-Ser-Ala-Lys-Phe-Pro-Ile-NH 2的肽 ，其与抗α-MSH和CRF的抗体具有交叉反应性，并且具有序列H-Gly-XNGE-Phe-Pro-Ala-Glu-Asn-Gly-Val-Gln-Asn-Thr-Glu-Ser -Thr-Gln-Glu-OH，其中XNGE是Pro-Ala-Val或Ser-Val-Ala，其与抗GRF的抗体交叉反应。

公开(公告)号：US4826680A

公开(公告)日：1989-05-02

申请号：US922001

申请日：1986-10-22

Applicant: Karl-Heinz Jaeger

Inventor： Karl-Heinz Jaeger

IPC: A61K35/26 ,  A61K38/00 ,  A61P31/12 ,  A61P37/04 ,  A61K37/02 ,  A61K31/525 ,  C07K7/48

CPC classification number: A61K35/26 ,  Y10S530/854

Abstract: A pharmaceutical composition is described, which contains a fraction consisting of low molecular proteins and/or oligo peptides, and a yellow fraction containing riboflavine, which is associated with organ specific oligo peptides, which fractions are obtainable by fractionation of thymus extract. This composition has a significant immuno stimulating activity, and can be used for the treatment of immuno deficiency diseases, as for example, for T-cell-deficiencies.

Abstract translation: 描述了一种药物组合物，其含有由低分子蛋白质和/或寡肽组成的级分，以及与器官特异性寡肽相关的含有核黄素的黄色级分，所述级分可通过分离胸腺提取物获得。 该组合物具有显着的免疫刺激活性，可用于治疗免疫缺陷疾病，例如T细胞缺陷。

公开(公告)号：US4717717A

公开(公告)日：1988-01-05

申请号：US927627

申请日：1986-11-05

Applicant: Amy L. Finkenaur

Inventor： Amy L. Finkenaur

IPC: A61K9/00 ,  A61K38/18 ,  A61K45/08 ,  A61K47/38 ,  A61L15/32 ,  A61L17/00 ,  C07K13/00 ,  A61K35/22 ,  A61K37/36

CPC classification number: A61K38/1808 ,  A61K47/38 ,  A61K9/0048 ,  A61L15/32 ,  A61L17/005 ,  A61L2300/414 ,  A61L2300/802 ,  Y10S530/808 ,  Y10S530/834 ,  Y10S530/854

Abstract: Medicinal compositions containing epidermal growth factor are stabilized against loss of biological activity in the presence of moisture by including in said compositions a stabilizing amount of water-soluble cellulose polymer, preferably methylcellulose or a hydroxy alkylcellulose such as hydroxypropyl methylcellulose.

Abstract translation: 含有表皮生长因子的药物组合物通过在所述组合物中包含稳定量的水溶性纤维素聚合物，优选甲基纤维素或羟基烷基纤维素如羟丙基甲基纤维素来稳定，防止在水分存在下丧失生物活性。

公开(公告)号：US4503035A

公开(公告)日：1985-03-05

申请号：US465979

申请日：1983-02-14

Applicant: Sidney Pestka ,  Menachem Rubinstein

Inventor： Sidney Pestka ,  Menachem Rubinstein

IPC: C07K1/20 ,  C07K14/56 ,  C07K14/665 ,  A61K45/02 ,  C07C103/52 ,  C12P21/00

CPC classification number: C07K14/56 ,  C07K1/20 ,  C07K14/665 ,  Y10S435/811 ,  Y10S530/828 ,  Y10S530/829 ,  Y10S530/854 ,  Y10S930/142

Abstract: An improved process for purifying proteins, particularly proteins having molecular weights in excess of 12,000 involves novel applications of high performance liquid chromatography on a preparative scale to provide homogeneous end product in excellent yield. In an embodiment of the process, human interferon is produced as a homogeneous protein.

Abstract translation: 用于纯化蛋白质，特别是分子量超过12,000的蛋白质的改进方法包括在制备规模上的高效液相色谱的新型应用，以提供优异的产率的均质终产物。 在该方法的一个实施方案中，人干扰素被产生为均匀的蛋白质。

公开(公告)号：US4427783A

公开(公告)日：1984-01-24

申请号：US330402

申请日：1981-12-14

Applicant: Edward Newman ,  A. Hirotoshi Nishikawa ,  Herbert E. Spiegel ,  Julia Symington

Inventor： Edward Newman ,  A. Hirotoshi Nishikawa ,  Herbert E. Spiegel ,  Julia Symington

IPC: G01N33/74 ,  G01N33/54 ,  G01N33/56

CPC classification number: G01N33/74 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/971 ,  Y10S435/972 ,  Y10S436/804 ,  Y10S436/811 ,  Y10S436/817 ,  Y10S530/806 ,  Y10S530/854

Abstract: An improved immunoassay for the polypeptide hormone thymosin .alpha..sub.1 is described. The assay employs an improved antibody which is elicited by an immunogen which has been prepared by coupling [Tyr.sup.1 ]-thymosin .alpha..sub.1 to an immunogenic carrier protein using a bifunctional diazonium coupling agent.

Abstract translation: 描述了多肽激素胸腺素α1的改进的免疫测定。 该测定采用由免疫原引发的改进的抗体，其通过使用双功能重氮偶联剂将[Tyr1] - 胸腺素α1与免疫原性载体蛋白偶联而制备。

公开(公告)号：US4371462A

公开(公告)日：1983-02-01

申请号：US341216

申请日：1982-01-21

Applicant: Randy I. Hecht

Inventor： Randy I. Hecht

IPC: A61K35/30 ,  A61K38/00 ,  A61K38/22 ,  A61K38/24 ,  A61K38/27 ,  C07K14/575 ,  C07K14/61 ,  A61K35/55 ,  C07G7/00

CPC classification number: C07K14/61 ,  C07K14/575 ,  A61K38/00 ,  Y10S530/854

Abstract: Mixtures containing an anterior pituitary hormone such as growth hormone or prolactin are treated to produce relatively pure hormone by a process comprising eluting the mixture through an ion-exchange column followed by fractionation of the eluent on an isoelectric focusing column. Fractions at the isoelectric point of the specific anterior pituitary hormone can be pooled, dialyzed with sterile water, and then lyophilized. The identity of the resulting product can be confirmed by electrophoretic analysis on sodium dodecylsulfate gel, immunodiffusion on an Ouchterlony plate, or both.

Abstract translation: 含有垂体前叶激素例如生长激素或催乳素的混合物通过包括通过离子交换柱洗脱该混合物然后在等电聚焦柱上分离洗脱液的方法处理以产生相对纯的激素。 可以将特定垂体前叶激素的等电点的级分合并，用无菌水透析，然后冻干。 所得产物的身份可以通过十二烷基硫酸钠凝胶的电泳分析，在Ouchterlony板上的免疫扩散或两者来确认。