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    Vectors and methods for immunization or therapeutic protocols
    1.
    发明授权
    Vectors and methods for immunization or therapeutic protocols 失效
    载体和免疫方法或治疗方案

    公开(公告)号：US06821957B2

    公开(公告)日：2004-11-23

    申请号：US09965101

    申请日：2001-09-26

    申请人： Arthur M. Krieg ,  Heather L. Davis ,  Tong Wu ,  Schorr Joachim

    发明人： Arthur M. Krieg ,  Heather L. Davis ,  Tong Wu ,  Schorr Joachim

    IPC分类号： A61K4800

    CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/292 ,  A61K39/39 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/55561 ,  A61K2039/57 ,  C12N2710/10343 ,  C12N2730/10134

    摘要： The present invention shows that DNA vaccine vectors can be improved by removal of CpG-N motifs and optional addition of CpG-S motifs. In addition, for high and long-lasting levels of expression, the optimized vector should include a promoter/enhancer that is not down-regulated by the cytokines induced by the immunostimulatory CpG motifs. Vectors and methods of use for immununostimulation are provided herein. The invention also provides improved gene therapy vectors by determining the CpG-N and CpG-S motifs present in the construct, removing stimulatory CpG (CpG-S) motifs and/or inserting neutralizing CpG (CpG-N) motifs, thereby producing a nucleic acid construct providing enhanced expression of the therapeutic polypeptide. Methods of use for such vectors are also included herein.

    摘要翻译： 本发明显示可以通过除去CpG-N基序和任选添加CpG-S基序来改善DNA疫苗载体。 此外，对于高和长期的表达水平，优化的载体应包括不被免疫刺激性CpG基序诱导的细胞因子下调的启动子/增强子。 本文提供了用于免疫刺激的载体和方法。 本发明还通过确定构建体中存在的CpG-N和CpG-S基序，除去刺激性CpG（CpG-S）基序和/或插入中和CpG（CpG-N）基序，从而产生核酸 提供治疗性多肽的增强的表达。 这些载体的使用方法也包括在本文中。

    Vectors and methods for immunization or therapeutic protocols
    2.
    发明授权
    Vectors and methods for immunization or therapeutic protocols 失效
    载体和免疫方法或治疗方案

    公开(公告)号：US06339068B1

    公开(公告)日：2002-01-15

    申请号：US09082649

    申请日：1998-05-20

    申请人： Arthur M. Krieg ,  Heather L. Davis ,  Tong Wu ,  Joachim Schorr

    发明人： Arthur M. Krieg ,  Heather L. Davis ,  Tong Wu ,  Joachim Schorr

    IPC分类号： A61K4800

    CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/292 ,  A61K39/39 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/55561 ,  A61K2039/57 ,  C12N2710/10343 ,  C12N2730/10134

    摘要： The present invention shows that DNA vaccine vectors can be improved by removal of CpG-N motifs and optional addition of CpG-S motifs. In addition, for high and long-lasting levels of expression, the optimized vector should include a promoter/enhancer that is not down-regulated by the cytokines induced by the immunostimulatory CpG motifs. Vectors and methods of use for immunostimulation are provided herein. The invention also provides improved gene therapy vectors by determining the CpG-N and CpG-S motifs present in the construct, removing stimulatory CpG (CpG-S) motifs and/or inserting neutralizing CpG (CpG-N) motifs, thereby producing a nucleic acid construct providing enhanced expression of the therapeutic polypeptide. Methods of use for such vectors are also included herein.

    摘要翻译： 本发明显示可以通过除去CpG-N基序和任选添加CpG-S基序来改善DNA疫苗载体。 此外，对于高和长期的表达水平，优化的载体应包括不被免疫刺激性CpG基序诱导的细胞因子下调的启动子/增强子。 本文提供了用于免疫刺激的载体和方法。 本发明还通过确定构建体中存在的CpG-N和CpG-S基序，除去刺激性CpG（CpG-S）基序和/或插入中和CpG（CpG-N）基序，从而产生核酸 提供治疗性多肽的增强的表达。 这些载体的使用方法也包括在本文中。