公开(公告)号：US20210388037A1

公开(公告)日：2021-12-16

申请号：US17293240

申请日：2019-11-18

Applicant: BASF SE

Inventor： Jingping Zhong ,  Xuqiu Tan ,  Melissa Ann Scranton

IPC: C07K14/39 ,  C12N15/81 ,  C12N1/16 ,  C12N9/20 ,  C12N9/24 ,  C12N9/30

Abstract: The present invention relates to a leader peptide which promotes the secretion of recombinant proteins and a nucleic acid sequence encoding the leader peptide as well as expression cassettes, vectors and host cells comprising this leader sequence. Also disclosed is a method for producing a protein using this leader peptide.

公开(公告)号：US20220002738A1

公开(公告)日：2022-01-06

申请号：US17293109

申请日：2019-11-18

Applicant: Jingping ZHONG ,  BASF SE

Inventor： Jingping Zhong ,  Xuqiu Tan ,  Melissa Ann Scranton ,  Daphne Li

IPC: C12N15/81 ,  C12P21/02 ,  C12N1/16 ,  C12N15/113

Abstract: The present invention relates to a recombinant yeast cell for high yield protein expression. The invention further relates to cell culture involving the recombinant yeast cell, a method for preparing protein involving culturing the recombinant yeast cell and a use of the recombinant yeast cell.

公开(公告)号：US20210115422A1

公开(公告)日：2021-04-22

申请号：US17051937

申请日：2019-04-24

Applicant: BASF SE

Inventor： Asfia Qureshi ,  Adrienne Huston Davenport ,  Xuqiu Tan ,  Oliver Spangenberg ,  Amanda Rae Logue ,  Janina Berndt ,  Tong Li ,  Stefan Seemayer ,  Hans Wolfgang Hoeffken ,  Jingping Zhong

IPC: C12N9/28 ,  A21D8/04

Abstract: Engineered variant polypeptides having amylase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. The genetically engineered amylase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, and ethanol production.

公开(公告)号：US20240084084A1

公开(公告)日：2024-03-14

申请号：US18512485

申请日：2023-11-17

Applicant: BASF SE

Inventor： Xuqiu Tan ,  Ruorong Cai ,  Jingping Zhong ,  Melissa Ann Scranton ,  Michael Speer

IPC: C08J5/18 ,  B29C48/00 ,  B29C48/08 ,  B29C65/02 ,  B65B9/02 ,  C08L23/08

CPC classification number: C08J5/18 ,  B29C48/022 ,  B29C48/08 ,  B29C65/02 ,  B65B9/023 ,  C08L23/0815 ,  B29K2023/06 ,  B29L2031/7128

Abstract: A promoter operably linked to a gene encoding a protein is disclosed. The promoter drives expression of the protein in a yeast cell in the absence of methanol. Also disclosed are vectors, host cells and expression systems that include the promoter, as well as methods of using the promoter to express proteins in yeast.

公开(公告)号：US11866714B2

公开(公告)日：2024-01-09

申请号：US15734682

申请日：2019-06-03

Applicant: BASF SE

Inventor： Xuqiu Tan ,  Ruorong Cai ,  Jingping Zhong ,  Melissa Ann Scranton ,  Michael Speer

IPC: C12N15/81 ,  C07K14/39

CPC classification number: C12N15/815 ,  C07K14/39

Abstract: A promoter operably linked to a gene encoding a protein is disclosed. The promoter drives expression of the protein in a yeast cell in the absence of methanol. Also disclosed are vectors, host cells and expression systems that include the promoter, as well as methods of using the promoter to express proteins in yeast.