公开(公告)号：US20210388037A1

公开(公告)日：2021-12-16

申请号：US17293240

申请日：2019-11-18

Applicant: BASF SE

Inventor： Jingping Zhong ,  Xuqiu Tan ,  Melissa Ann Scranton

IPC: C07K14/39 ,  C12N15/81 ,  C12N1/16 ,  C12N9/20 ,  C12N9/24 ,  C12N9/30

Abstract: The present invention relates to a leader peptide which promotes the secretion of recombinant proteins and a nucleic acid sequence encoding the leader peptide as well as expression cassettes, vectors and host cells comprising this leader sequence. Also disclosed is a method for producing a protein using this leader peptide.

公开(公告)号：US20210095266A1

公开(公告)日：2021-04-01

申请号：US17050117

申请日：2019-04-24

Applicant: BASF SE

Inventor： Katie Kline ,  Adrienne Huston Davenport ,  Xuqiu Tan ,  Oliver Spangenberg ,  Claudia Esper ,  Amy Migliori

IPC: C12N9/20 ,  C11D3/386

Abstract: Engineered variant polypeptides having lipase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. The genetically engineered lipase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, biodiesel and ethanol production.

公开(公告)号：US20230212544A1

公开(公告)日：2023-07-06

申请号：US18086034

申请日：2022-12-21

Applicant: BASF SE

Inventor： Hugo Urbina ,  Tong Li ,  Jared Dennis ,  Xuqiu Tan ,  Adrienne Huston Davenport ,  Jochen Kutscher ,  Kristine W. Chang

IPC: C12N9/26

CPC classification number: C12N9/2425 ,  C12Y302/01002

Abstract: Variant polypeptides having beta-amylase activity and methods of making and using the enzymes in baking, detergents, personal care products, in the processing of textiles, in pulp and paper processing, in the production of ethanol, lignocellulosic ethanol, or syrups; as viscosity breakers in oilfield and mining industries.

公开(公告)号：US11542487B2

公开(公告)日：2023-01-03

申请号：US16757993

申请日：2018-10-17

Applicant: BASF SE

Inventor： Hugo Urbina ,  Tong Li ,  Jared Dennis ,  Xuqiu Tan ,  Adrienne Huston Davenport ,  Jochen Kutscher ,  Kristine W. Chang

IPC: C12N9/26

Abstract: Variant polypeptides having beta-amylase activity and methods of making and using the enzymes in baking, detergents, personal care products, in the processing of textiles, in pulp and paper processing, in the production of ethanol, lignocellulosic ethanol, or syrups; as viscosity breakers in oilfield and mining industries.

公开(公告)号：US20220162576A1

公开(公告)日：2022-05-26

申请号：US17442489

申请日：2020-03-24

Applicant: BASF SE

Inventor： Stefan Jenewein ,  Zachary David Miles ,  Asfia Qureshi ,  Jared Dennis ,  Tong Li ,  Xuqiu Tan ,  Priya Anand ,  Jesper Nielsen

IPC: C12N9/28

Abstract: Genetically engineered enzymes having amylase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. Fragments of parent amylase enzymes activity and methods of using the fragments for making genetically engineered enzymes having amylase activity. The genetically engineered amylase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, brewing, and ethanol production.

公开(公告)号：US20220002738A1

公开(公告)日：2022-01-06

申请号：US17293109

申请日：2019-11-18

Applicant: Jingping ZHONG ,  BASF SE

Inventor： Jingping Zhong ,  Xuqiu Tan ,  Melissa Ann Scranton ,  Daphne Li

IPC: C12N15/81 ,  C12P21/02 ,  C12N1/16 ,  C12N15/113

Abstract: The present invention relates to a recombinant yeast cell for high yield protein expression. The invention further relates to cell culture involving the recombinant yeast cell, a method for preparing protein involving culturing the recombinant yeast cell and a use of the recombinant yeast cell.

公开(公告)号：US20210115422A1

公开(公告)日：2021-04-22

申请号：US17051937

申请日：2019-04-24

Applicant: BASF SE

Inventor： Asfia Qureshi ,  Adrienne Huston Davenport ,  Xuqiu Tan ,  Oliver Spangenberg ,  Amanda Rae Logue ,  Janina Berndt ,  Tong Li ,  Stefan Seemayer ,  Hans Wolfgang Hoeffken ,  Jingping Zhong

IPC: C12N9/28 ,  A21D8/04

Abstract: Engineered variant polypeptides having amylase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. The genetically engineered amylase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, and ethanol production.

公开(公告)号：US12110489B2

公开(公告)日：2024-10-08

申请号：US16954092

申请日：2018-12-18

Applicant: BASF SE

Inventor： Jared Dennis ,  Xuqiu Tan ,  Robin Cai

IPC: C12N15/10 ,  C12N1/20 ,  C12N15/70

CPC classification number: C12N15/1031 ,  C12N1/20 ,  C12N15/70 ,  C12N2800/101

Abstract: Disclosed herein is an improved Gene Site Saturation Mutagenesis (GSSM) method for producing a plurality of modified polynucleotides and/or polypeptides, creating specific changes to a gene, and reassembling mutations or changes at one or more sites.

公开(公告)号：US11634698B2

公开(公告)日：2023-04-25

申请号：US16954276

申请日：2018-12-10

Applicant: BASF SE

Inventor： Xuqiu Tan ,  Kenneth Barrett ,  Jared Dennis ,  Dongmei Ren ,  Corey Dodge ,  Bin Zhang

IPC: C12N9/22 ,  A23K20/147 ,  A23K20/189 ,  A23L29/00 ,  A23L33/18 ,  C11D3/386

Abstract: Disclosed herein are polypeptides having nuclease activity. Some of the polypeptides having nuclease activity were generated by an improved gene site mutagenesis (“GSSM”) method or the tailored multi-site combinatorial assembly (“TMCA”) method. Also disclosed are compositions and kits comprising the polypeptides having nuclease activity, and methods for making and using these polypeptides, compositions and kits.

公开(公告)号：US20220170001A1

公开(公告)日：2022-06-02

申请号：US17442675

申请日：2020-03-24

Applicant: BASF SE

Inventor： Asfia Qureshi ,  Zachary David Miles ,  Xuqiu Tan ,  Tong Li ,  Jared Dennis ,  Stefan Jenewein ,  Priya Anand ,  Jesper Nielsen

IPC: C12N9/26 ,  C12N1/20 ,  C12N15/75 ,  A21D8/04

Abstract: Genetically engineered enzymes having amylase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. Fragments of parent amylase enzymes activity and methods of using the fragments for making genetically engineered enzymes having amylase activity. The genetically engineered amylase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, brewing, and ethanol production.