公开(公告)号：US20150147800A1

公开(公告)日：2015-05-28

申请号：US14584289

申请日：2014-12-29

Applicant: BASF SE

Inventor： Bernhard Hauer ,  Nina Schneider ,  Krzysztof Okrasa ,  Jason Micklefield ,  David Leys ,  Colin Levy

IPC: C12N9/88

CPC classification number: C12N9/88 ,  C07K14/235 ,  C07K2299/00 ,  C12N9/90 ,  C12P7/40 ,  C12P7/42 ,  C12Q1/00 ,  C12Y401/01075 ,  C12Y401/01076 ,  G01N2333/235 ,  G01N2500/00

Abstract: The present invention relates to a method for the enzymatic decarboxylation of malonic acid (propanedioic acid) derivatives catalyzed by enzymes structurally and/or functionally related to arylmalonate decarboxylase (AMDase) as isolated from microorganisms of the genus Bordetella. The present invention also relates to novel enzymes with a decarboxylase activity, useful for performing the claimed method, mutants thereof, corresponding coding sequences and expression systems, methods of preparing said novel enzymes, and screening methods for obtaining further suitable enzymes also having said decarboxylase activity.

Abstract translation: 本发明涉及由结构和/或与芳基丙二酸酯脱羧酶（AMDase）功能相关的酶催化的由在博德特氏菌属微生物分离的酶催化的丙二酸（丙二酸）衍生物的酶脱羧的方法。 本发明还涉及具有脱羧酶活性的新型酶，可用于实施要求保护的方法，其突变体，相应的编码序列和表达系统，制备所述新型酶的方法，以及筛选获得还具有所述脱羧酶活性的合适酶的筛选方法 。