公开(公告)号：US20190024038A1

公开(公告)日：2019-01-24

申请号：US16060626

申请日：2016-12-08

Applicant: BASF SE

Inventor： Katrin Treier-Marxen ,  Petra Deckert ,  Andreas Schaedler ,  Vaidotas Navickas ,  Volker Wengert ,  Thomas Kaeding

IPC: C12N1/02 ,  B01D61/14 ,  B01D61/16 ,  C07K1/14 ,  C07K1/30 ,  C12P21/02

Abstract: The present invention is directed to a method of purifying a protein of interest from the particulate matter of a fermentation broth comprising the step of purifying the protein of interest from the particulate matter of the fermentation broth, wherein the step of purifying the protein of interest from the particulate matter of the fermentation broth comprises one or more washing steps, which comprises contacting the particulate matter with a washing solution comprising one or more conditions that favor the solubilization of the protein of interest and/or the desorption of the protein of interest from the particulate matter.

公开(公告)号：US09493405B2

公开(公告)日：2016-11-15

申请号：US14941167

申请日：2015-11-13

Applicant: BASF SE

Inventor： Tim Jungkamp ,  Robert Baumann ,  Michael Bartsch ,  Gerd Haderlein ,  Hermann Luyken ,  Jens Scheidel ,  Tobias Aechtner ,  Peter Pfab ,  Petra Deckert ,  Wolfgang Siegel ,  Peter Bassler

IPC: C07C253/30 ,  C07C253/10

CPC classification number: C07C253/30 ,  C07C253/10 ,  Y02P20/582 ,  C07C255/07

Abstract: A process is described for preparing 3-pentenenitrile, characterized by the following process steps: (a) isomerizing a reactant stream which comprises 2-methyl-3-butenenitrile over at least one dissolved or dispersed isomerization catalyst to give a stream 1 which comprises the at least one isomerization catalyst, 2-methyl-3-butenenitrile, 3-pentenenitrile and (Z)-2-methyl-2-butenenitrile, (b) distilling stream 1 to obtain a stream 2 as the top product which comprises 2-methyl-3-butenenitrile, 3-pentenenitrile and (Z)-2-methyl-2-butenenitrile, and a stream 3 as the bottom product which comprises the at least one isomerization catalyst, (c) distilling stream 2 to obtain a stream 4 as the top product which, compared to stream 2, is enriched in (Z)-2-methyl-2-butenenitrile, based on the sum of all pentenenitriles in stream 2, and a stream 5 as the bottom product which, compared to stream 2, is enriched in 3-pentenenitrile and 2-methyl-3-butenenitrile, based on the sum of all pentenenitriles in stream 2, (d) distilling stream 5 to obtain a stream 6 as the bottom product which comprises 3-pentenenitrile and a stream 7 as the top product which comprises 2-methyl-3-butenenitrile.

公开(公告)号：US20240191175A1

公开(公告)日：2024-06-13

申请号：US18443753

申请日：2024-02-16

Applicant: BASF SE

Inventor： Katrin Treier-Marxen ,  Petra Deckert ,  Andreas Schaedler ,  Vaidotas Navickas ,  Volker Wengert ,  Thomas Kaeding

IPC: C12N1/02 ,  B01D61/14 ,  B01D61/16 ,  C07K1/14 ,  C07K1/30 ,  C12P21/02

CPC classification number: C12N1/02 ,  B01D61/147 ,  B01D61/16 ,  C07K1/145 ,  C07K1/30 ,  C12P21/02 ,  B01D2311/04 ,  B01D2311/18 ,  B01D2315/16

Abstract: The present invention is directed to a method of purifying a protein of interest from the particulate matter of a fermentation broth comprising the step of purifying the protein of interest from the particulate matter of the fermentation broth, wherein the step of purifying the protein of interest from the particulate matter of the fermentation broth comprises one or more washing steps, which comprises contacting the particulate matter with a washing solution comprising one or more conditions that favor the solubilization of the protein of interest and/or the desorption of the protein of interest from the particulate matter.