公开(公告)号：US20220275355A1

公开(公告)日：2022-09-01

申请号：US17615160

申请日：2020-06-09

Applicant: BASF SE

Inventor： Katrin Treier-Marxen ,  Sebastian Schoof ,  Andreas Schaedler ,  Stephan Freyer ,  Michael Helmut Kopf

IPC: C12N9/28 ,  C12N1/20 ,  C07K1/14

Abstract: The present invention relates to a method for recovering a protein of interest from a fermentation broth by adding a salt of a divalent cation and increasing the pH.

公开(公告)号：US12146171B2

公开(公告)日：2024-11-19

申请号：US17615160

申请日：2020-06-09

Applicant: BASF SE

Inventor： Katrin Treier-Marxen ,  Sebastian Schoof ,  Andreas Schaedler ,  Stephan Freyer ,  Michael Helmut Kopf

IPC: C12N9/00 ,  C07K1/14 ,  C12N1/20 ,  C12N9/28 ,  C12R1/10

Abstract: The present invention relates to a method for recovering a protein of interest from a fermentation broth by adding a salt of a divalent cation and increasing the pH.

公开(公告)号：US20240191175A1

公开(公告)日：2024-06-13

申请号：US18443753

申请日：2024-02-16

Applicant: BASF SE

Inventor： Katrin Treier-Marxen ,  Petra Deckert ,  Andreas Schaedler ,  Vaidotas Navickas ,  Volker Wengert ,  Thomas Kaeding

IPC: C12N1/02 ,  B01D61/14 ,  B01D61/16 ,  C07K1/14 ,  C07K1/30 ,  C12P21/02

CPC classification number: C12N1/02 ,  B01D61/147 ,  B01D61/16 ,  C07K1/145 ,  C07K1/30 ,  C12P21/02 ,  B01D2311/04 ,  B01D2311/18 ,  B01D2315/16

Abstract: The present invention is directed to a method of purifying a protein of interest from the particulate matter of a fermentation broth comprising the step of purifying the protein of interest from the particulate matter of the fermentation broth, wherein the step of purifying the protein of interest from the particulate matter of the fermentation broth comprises one or more washing steps, which comprises contacting the particulate matter with a washing solution comprising one or more conditions that favor the solubilization of the protein of interest and/or the desorption of the protein of interest from the particulate matter.

公开(公告)号：US20190024038A1

公开(公告)日：2019-01-24

申请号：US16060626

申请日：2016-12-08

Applicant: BASF SE

Inventor： Katrin Treier-Marxen ,  Petra Deckert ,  Andreas Schaedler ,  Vaidotas Navickas ,  Volker Wengert ,  Thomas Kaeding

IPC: C12N1/02 ,  B01D61/14 ,  B01D61/16 ,  C07K1/14 ,  C07K1/30 ,  C12P21/02

Abstract: The present invention is directed to a method of purifying a protein of interest from the particulate matter of a fermentation broth comprising the step of purifying the protein of interest from the particulate matter of the fermentation broth, wherein the step of purifying the protein of interest from the particulate matter of the fermentation broth comprises one or more washing steps, which comprises contacting the particulate matter with a washing solution comprising one or more conditions that favor the solubilization of the protein of interest and/or the desorption of the protein of interest from the particulate matter.