公开(公告)号：US20230331775A1

公开(公告)日：2023-10-19

申请号：US18026860

申请日：2021-09-21

Applicant: BASF SE

Inventor： Sebastian Schoof ,  Ronja Muenkel

IPC: C07K1/34 ,  C12N1/20

CPC classification number: C07K1/34 ,  C12N1/205 ,  C12R2001/10

Abstract: The present invention relates to a method for recovering a protein of interest from a bacterial fermentation broth, wherein the bacterial cells in the fermentation broth exhibit a high degree of lysis, comprising the steps of adding to the fermentation broth comprising the protein of interest at least one flocculant in an amount of 0.5 to 50 g/l of the fermentation broth; separating the protein of interest from at least the bacterial cells by filtration, preferably by dead-end filtration or microfiltration; and thereby obtaining the protein of interest in the filtrate; wherein the bacterial cells are Bacillus cells. Further, the invention relates to the use of a flocculant for recovering a protein of interest from a bacterial fermentation broth comprising a high degree of lysed cells.

公开(公告)号：US20230323329A1

公开(公告)日：2023-10-12

申请号：US18023130

申请日：2021-09-03

Applicant: BASF SE

Inventor： Sebastian Schoof ,  Thomas Kaeding ,  Ronja Muenkel

IPC: C12N9/54 ,  C12N9/14 ,  C12P21/02

CPC classification number: C12N9/54 ,  C12P21/02 ,  C12Y304/21062 ,  C12N9/14

Abstract: The present invention relates to a method for separating a molecule of interest from an antifoaming agent in a fermentation broth comprising said molecule of interest and said antifoaming agent. The method comprises the steps of filtering the fermentation broth at a temperature in the range of 1° C. to 15° C. above the cloud point of the antifoaming agent, and thereby obtaining a first fraction of the fermentation broth comprising the molecule of interest and a second fraction of the fermentation broth comprising the antifoaming agent, wherein the antifoaming agent is a polyalkylene glycol based antifoaming agent. The invention further relates to a process for purifying a molecule of interest from a fermentation broth comprising said method.