公开(公告)号：US20250051822A1

公开(公告)日：2025-02-13

申请号：US18720123

申请日：2022-12-19

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Christopher Sauer ,  Mathis Appelbaum ,  Stefan Jenewein ,  Sebastian Schoof ,  Christian Riedele

IPC: C12P21/02 ,  C12N9/38 ,  C12N15/75

Abstract: The present invention relates to a method for producing at least one polypeptide of interest in a Bacillus host cell, said method comprising the steps of a) providing a Bacillus host cell comprising a polynucleotide encoding the at least one polypeptide of interest, b) cultivating the Bacillus host cell under conditions which allow for intracellular accumulation of said at least one polypeptide of interest, thereby producing said at least one polypeptide of interest, c) stop of aeration and addition of feed, and d) incubating the cells for at least hours. Moreover, the present invention relates to a Bacillus host cell comprising a polynucleotide encoding at least one polypeptide of interest lacking a functional secretion sequence, wherein the ispA gene has been inactivated in the Bacillus host cell.

公开(公告)号：US20220275355A1

公开(公告)日：2022-09-01

申请号：US17615160

申请日：2020-06-09

Applicant: BASF SE

Inventor： Katrin Treier-Marxen ,  Sebastian Schoof ,  Andreas Schaedler ,  Stephan Freyer ,  Michael Helmut Kopf

IPC: C12N9/28 ,  C12N1/20 ,  C07K1/14

Abstract: The present invention relates to a method for recovering a protein of interest from a fermentation broth by adding a salt of a divalent cation and increasing the pH.

公开(公告)号：US20230331775A1

公开(公告)日：2023-10-19

申请号：US18026860

申请日：2021-09-21

Applicant: BASF SE

Inventor： Sebastian Schoof ,  Ronja Muenkel

IPC: C07K1/34 ,  C12N1/20

CPC classification number: C07K1/34 ,  C12N1/205 ,  C12R2001/10

Abstract: The present invention relates to a method for recovering a protein of interest from a bacterial fermentation broth, wherein the bacterial cells in the fermentation broth exhibit a high degree of lysis, comprising the steps of adding to the fermentation broth comprising the protein of interest at least one flocculant in an amount of 0.5 to 50 g/l of the fermentation broth; separating the protein of interest from at least the bacterial cells by filtration, preferably by dead-end filtration or microfiltration; and thereby obtaining the protein of interest in the filtrate; wherein the bacterial cells are Bacillus cells. Further, the invention relates to the use of a flocculant for recovering a protein of interest from a bacterial fermentation broth comprising a high degree of lysed cells.

公开(公告)号：US12146171B2

公开(公告)日：2024-11-19

申请号：US17615160

申请日：2020-06-09

Applicant: BASF SE

Inventor： Katrin Treier-Marxen ,  Sebastian Schoof ,  Andreas Schaedler ,  Stephan Freyer ,  Michael Helmut Kopf

IPC: C12N9/00 ,  C07K1/14 ,  C12N1/20 ,  C12N9/28 ,  C12R1/10

Abstract: The present invention relates to a method for recovering a protein of interest from a fermentation broth by adding a salt of a divalent cation and increasing the pH.

公开(公告)号：US20230323329A1

公开(公告)日：2023-10-12

申请号：US18023130

申请日：2021-09-03

Applicant: BASF SE

Inventor： Sebastian Schoof ,  Thomas Kaeding ,  Ronja Muenkel

IPC: C12N9/54 ,  C12N9/14 ,  C12P21/02

CPC classification number: C12N9/54 ,  C12P21/02 ,  C12Y304/21062 ,  C12N9/14

Abstract: The present invention relates to a method for separating a molecule of interest from an antifoaming agent in a fermentation broth comprising said molecule of interest and said antifoaming agent. The method comprises the steps of filtering the fermentation broth at a temperature in the range of 1° C. to 15° C. above the cloud point of the antifoaming agent, and thereby obtaining a first fraction of the fermentation broth comprising the molecule of interest and a second fraction of the fermentation broth comprising the antifoaming agent, wherein the antifoaming agent is a polyalkylene glycol based antifoaming agent. The invention further relates to a process for purifying a molecule of interest from a fermentation broth comprising said method.