LIGHT MICROSCOPY METHOD, DEVICE AND COMPUTER PROGRAM PRODUCT

    公开(公告)号:US20240346810A1

    公开(公告)日:2024-10-17

    申请号:US18619652

    申请日:2024-03-28

    Abstract: The disclosure relates to a light microscopy method comprising acquiring first light microscopic data of a sample in a first acquisition mode, recognizing an object in the sample from the first light microscopic data and assigning the object to an object class using a first artificial intelligence method, determining a confidence value for the recognized object, comparing the confidence value with a predetermined confidence value threshold if the confidence value is below the confidence value threshold, acquiring second light microscopic data in a second acquisition mode and verifying the assignment of the object to the object class based on the second light microscopic data, as well as a device and a computer program product for carrying out the method.

    PROCESSOR FOR LIFETIME-BASED UNMIXING IN FLUORESCENCE MICROSCOPY

    公开(公告)号:US20240290117A1

    公开(公告)日:2024-08-29

    申请号:US18586622

    申请日:2024-02-26

    Abstract: A processor for lifetime-based unmixing in fluorescence microscopy is configured to acquire an image having a plurality of pixels, each pixel providing information on photon count and photon arrival times, generate a phasor plot that is a vector space representation of the image, partition the image into image segments, evaluate the image segments according to total photon counts of the corresponding subsets of pixels, and execute a lifetime classification by selecting an image segment having a largest total photon count, determining a region of interest in the image encompassing the image segment, determining a phasor subset in the phasor plot corresponding to the region of interest, and generating a lifetime class including a set of image segments corresponding to the phasor subset. A plurality of lifetime classes is generated by iteratively executing the lifetime classification. The processor is configured to perform lifetime-based unmixing using the life-time classes.

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