Methods of small sample amplification
    2.
    发明申请
    Methods of small sample amplification 审中-公开
    小样本扩增方法

    公开(公告)号:US20050003392A1

    公开(公告)日:2005-01-06

    申请号:US10805559

    申请日:2004-03-19

    IPC分类号: C12P19/34 C12Q1/68

    CPC分类号: C12P19/34 C12Q1/68

    摘要: The present invention relates to the amplification of nucleic acids, preferably from mRNA. A primer and promoter are added to a target sequence to be amplified and then the target is amplified in an in vitro transcription reaction and the product of this reaction is used as template for subsequent rounds of amplification. Polyadenylated control transcripts are added to the nucleic acid sample prior to the first step of amplification to monitor the efficiency of the amplification and labeling reactions.

    摘要翻译: 本发明涉及核酸,优选mRNA的扩增。 将引物和启动子加入待扩增的靶序列中,然后在体外转录反应中扩增靶,并将该反应的产物用作随后的扩增循环。 将多腺苷酸化的对照转录物在扩增的第一步骤之前加入到核酸样品中,以监测扩增和标记反应的效率。