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公开(公告)号:US6096172A
公开(公告)日:2000-08-01
申请号:US174606
申请日:1998-10-19
申请人: Barbara Foley , Natalia Briones
发明人: Barbara Foley , Natalia Briones
IPC分类号: G01N33/53 , B01J19/00 , B01J19/12 , C07B61/00 , C12M1/00 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/543 , G01N33/553 , G01N33/566 , G01N37/00 , C07C1/00 , C12N11/00
CPC分类号: B82Y30/00 , B01J19/0046 , G01N33/54353 , G01N33/553 , B01J2219/00427 , B01J2219/005 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00711 , B01J2219/00713 , B01J2219/00716 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C40B40/06 , C40B40/10 , C40B60/14
摘要: A method of bonding bio-molecules to a test site including providing a substrate having a test site defined on a surface thereof, providing a solution containing a plurality of probe molecules and bonding material, directing light from a light source onto the test site so as to cause the bonding material to bond the probe molecules to the test site.
摘要翻译: 一种将生物分子粘合到测试部位的方法,包括提供在其表面上具有测试部位的基底,提供含有多个探针分子和粘合材料的溶液,将来自光源的光引导到测试部位,以便 以使结合材料将探针分子结合到测试部位。
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公开(公告)号:US20050003392A1
公开(公告)日:2005-01-06
申请号:US10805559
申请日:2004-03-19
申请人: Susana Salceda , Kai Wu , Natalia Briones , Qing Bai , Yanxiang Cao
发明人: Susana Salceda , Kai Wu , Natalia Briones , Qing Bai , Yanxiang Cao
摘要: The present invention relates to the amplification of nucleic acids, preferably from mRNA. A primer and promoter are added to a target sequence to be amplified and then the target is amplified in an in vitro transcription reaction and the product of this reaction is used as template for subsequent rounds of amplification. Polyadenylated control transcripts are added to the nucleic acid sample prior to the first step of amplification to monitor the efficiency of the amplification and labeling reactions.
摘要翻译: 本发明涉及核酸,优选mRNA的扩增。 将引物和启动子加入待扩增的靶序列中,然后在体外转录反应中扩增靶,并将该反应的产物用作随后的扩增循环。 将多腺苷酸化的对照转录物在扩增的第一步骤之前加入到核酸样品中,以监测扩增和标记反应的效率。
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