公开(公告)号：US20050124011A1

公开(公告)日：2005-06-09

申请号：US10896868

申请日：2004-07-23

申请人： Carol Morris ,  Mark Willcox ,  Shirley Bolis ,  Bradley Walsh ,  Ben Herbert ,  Mark Molloy ,  Andrew Gooley ,  Keith Williams

发明人： Carol Morris ,  Mark Willcox ,  Shirley Bolis ,  Bradley Walsh ,  Ben Herbert ,  Mark Molloy ,  Andrew Gooley ,  Keith Williams

IPC分类号： C07K14/47 ,  G01N33/574 ,  G01N33/53

CPC分类号： C07K14/47 ,  G01N33/57415 ,  G01N33/57434 ,  G01N33/57488

摘要： A method of screening or detecting non-ocular disease in an animal, the method comprising the steps of: (a) obtaining a tear sample from the animal; (b) separating biomolecules present in the tear sample; and (c) detecting for the presence or absence of one or more biomolecules such that the presence or absence of the one or more biomolecules being an indicator or marker of a disease state in the animal.

摘要翻译： 一种在动物中筛选或检测非眼部疾病的方法，所述方法包括以下步骤：（a）从动物获得泪液样品; （b）分离存在于泪液样品中的生物分子; 和（c）检测一种或多种生物分子的存在或不存在，使得一种或多种生物分子的存在或不存在是动物中疾病状态的指示剂或标志物。

公开(公告)号：US20080035483A1

公开(公告)日：2008-02-14

申请号：US11711808

申请日：2007-02-28

申请人： Ben Herbert ,  Andrew Gooley ,  Keith Williams

发明人： Ben Herbert ,  Andrew Gooley ,  Keith Williams

IPC分类号： B01D57/02

CPC分类号： C07K1/285 ,  G01N27/44747 ,  G01N27/44795

摘要： An improved method of separating a macromolecule by isoelectric focusing comprising subjecting the macromolecule to electrophoresis in an isoelectric-focusing medium including a substantially thiol-free reducing agent, preferably a trivalent phosphorous compound and more preferably tributyl phosphine, the improvement being the solubility and focusing of the macromolecule is enhanced compared to isoelectric focusing of the same macromolecule in a similar isoelectric-focusing medium containing a thiol-reducing agent.

摘要翻译： 通过等电聚焦分离大分子的改进方法包括使大分子在包括基本上不含硫醇的还原剂，优选三价磷化合物，更优选三丁基膦的等电聚焦介质中进行电泳，其改进是溶解度和聚焦 与含有硫醇还原剂的类似等电聚焦介质中的相同大分子的等电聚焦相比，大分子得到增强。

公开(公告)号：US06558522B1

公开(公告)日：2003-05-06

申请号：US09582260

申请日：2000-09-14

申请人： Keith Leslie Williams ,  Mark Molloy ,  Andrew Arthur Gooley ,  Nicolle Hannah Packer ,  Ben Herbert ,  Bradley Walsh ,  George Fernwood ,  David Garfin

发明人： Keith Leslie Williams ,  Mark Molloy ,  Andrew Arthur Gooley ,  Nicolle Hannah Packer ,  Ben Herbert ,  Bradley Walsh ,  George Fernwood ,  David Garfin

IPC分类号： B01D5702

CPC分类号： B01D57/02 ,  G01N27/44704 ,  G01N27/44782

摘要： An electrophoresis apparatus for the simultaneous electrophoretic separation of a plurality of component mixtures using a disposable tray having a plurality of parallel troughs shaped to retain an elongate strip of isoelectric focusing medium and sufficient liquid to immerse the elongate strip. A frame is provided to retain the tray in a horizontal position using a pair of rotatably mounted support members moving between an open and closed position. Support members are also connected to a plurality of electrodes aligned with each of the troughs so that the electrodes are positioned clear of the troughs in the open position and positioned in the troughs when in the closed position.

摘要翻译： 一种用于使用一次性盘的同时电泳分离多个组分混合物的电泳装置，所述一次性盘具有多个平行槽，所述多个平行槽被成形为保持等电聚焦介质的细长带和足够的液体以浸入细长条。 提供了一个框架，以使用一对可旋转地安装的支撑构件在打开和关闭位置之间移动来将托盘保持在水平位置。 支撑构件还连接到与每个槽对准的多个电极，使得电极在处于关闭位置时被定位成远离槽并且位于槽中。

公开(公告)号：US20120085646A1

公开(公告)日：2012-04-12

申请号：US13226170

申请日：2011-09-06

申请人： Ben Herbert ,  Andrew Arthur Gooley ,  Keith Leslie Williams

发明人： Ben Herbert ,  Andrew Arthur Gooley ,  Keith Leslie Williams

IPC分类号： C07K1/28 ,  B01D57/02

CPC分类号： C07K1/285 ,  G01N27/44747 ,  G01N27/44795

摘要： An improved method of separating a macromolecule by isoelectric focusing comprising subjecting the macromolecule to electrophoresis in an isoelectric-focusing medium including a substantially thiol-free reducing agent, preferably a trivalent phosphorous compound and more preferably tributyl phosphine, the improvement being the solubility and focusing of the macromolecule is enhanced compared to isoelectric focusing of the same macromolecule in a similar isoelectric-focusing medium containing a thiol-reducing agent.

摘要翻译： 通过等电聚焦分离大分子的改进方法包括使大分子在包括基本上不含硫醇的还原剂，优选三价磷化合物，更优选三丁基膦的等电聚焦介质中进行电泳，其改进是溶解度和聚焦 与含有硫醇还原剂的类似等电聚焦介质中的相同大分子的等电聚焦相比，大分子得到增强。

公开(公告)号：US06942774B1

公开(公告)日：2005-09-13

申请号：US10070914

申请日：2000-09-08

申请人： Ben Herbert

发明人： Ben Herbert

IPC分类号： G01N33/483 ,  B01D57/02 ,  B03C5/00 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/68 ,  G01N27/447 ,  G01N27/453

CPC分类号： G01N27/44704

摘要： An apparatus for rehydrating and for performing electrophoresis on a gel strip includes a tray defining a plurality of parallel troughs configured to receive gel strips. Each trough defines a centrally located rehydration area and an electrode area disposed either side of the electrode area. End walls delimit the rehydration area of the trough from the electrode area. Electrode means including contact points adapted to contact either the gel strip in the electrode areas near the first and second end of the gel or a conducting or current carrying electrode bridge material which is in contact with the gel strip in the electrode areas. The electrode means are adapted to be connected to a means for supplying an electric current for imposing an electric potential in the strip between the electrodes.

摘要翻译： 用于在凝胶条上再水合和进行电泳的装置包括限定多个平行槽的托盘，其配置成接收凝胶条。 每个槽限定位于中心的再水化区域和设置在电极区域的任一侧的电极区域。 端壁从电极区域界定槽的再水合区域。 电极是指包括适于接触凝胶的第一和第二端附近的电极区域中的凝胶条的接触点或与电极区域中的凝胶条接触的导电或载流电极桥接材料。 电极装置适于连接到用于在电极之间的条带中施加电位的电流的装置。

公开(公告)号：US20140114053A1

公开(公告)日：2014-04-24

申请号：US14135578

申请日：2013-12-20

申请人： Ben Herbert ,  Andrew Arthur Gooley ,  Keith Leslie Williams

发明人： Ben Herbert ,  Andrew Arthur Gooley ,  Keith Leslie Williams

IPC分类号： C07K1/28

CPC分类号： C07K1/285 ,  G01N27/44747 ,  G01N27/44795

摘要： An improved method of separating a macromolecule by isoelectric focusing comprising subjecting the macromolecule to electrophoresis in an isoelectric-focusing medium including a substantially thiol-free reducing agent, preferably a trivalent phosphorous compound and more preferably tributyl phosphine, the improvement being the solubility and focusing of the macromolecule is enhanced compared to isoelectric focusing of the same macromolecule in a similar isoelectric-focusing medium containing a thiol-reducing agent.

摘要翻译： 通过等电聚焦分离大分子的改进方法包括使大分子在包括基本上不含硫醇的还原剂，优选三价磷化合物，更优选三丁基膦的等电聚焦介质中进行电泳，其改进是溶解度和聚焦 与含有硫醇还原剂的类似等电聚焦介质中的相同大分子的等电聚焦相比，大分子得到增强。

公开(公告)号：US06905584B2

公开(公告)日：2005-06-14

申请号：US09966352

申请日：2001-09-28

申请人： Ben Herbert

发明人： Ben Herbert

IPC分类号： C07K1/26 ,  G01N27/447

CPC分类号： G01N27/44773 ,  C07K1/26 ,  C07K1/285 ,  G01N27/44795

摘要： A method for separating a sample into components by two-dimensional electrophoresis uses an IPG strip, and a gel slab which are spaced apart and carried on a single generally planar support means. The planar support means is first oriented in a generally vertical plane and the first electrophoretic separation medium is oriented in a horizontal plane spaced above or below the second electrophoretic separation medium by a gap. A first dimension separation of a sample mixture in the strip is then carried out while the strip and slab are separated by a non-electrically conducting liquid which is substantially immiscible with water and is non-extractive of water preferably paraffin oil. After the first separation has been carried the support means is tilted so that the first strip is at an angle to the horizontal and the paraffin is flushed out from the gap between the strip and the slab. Next agarose gel containing buffer is flowed into the gap to allow transfer of sample molecules from the strip to the slab under the influence of an electric field.

摘要翻译： 通过二维电泳将样品分离成组分的方法使用IPG条和间隔开并承载在单个大体上平面的支撑装置上的凝胶板。 平面支撑装置首先被定向在大致垂直的平面中，并且第一电泳分离介质在水平面上定向，间隔在第二电泳分离介质的上方或下方。 然后在条带和板坯由与水基本上不混溶的非导电液体分离的条件下进行样品混合物的第一尺寸分离，并且是非萃取性的水，优选石蜡油。 在第一分离已经被承载之后，支撑装置倾斜，使得第一条带与水平面成一定角度，并且石蜡从带材和板坯之间的间隙中冲出。 将下一个含有琼脂糖凝胶的缓冲液流入间隙，以允许样品分子在电场的影响下从条带转移到板坯。