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    Use of nucleic acid analogues in diagnostics and analytical procedures
    3.
    发明授权
    Use of nucleic acid analogues in diagnostics and analytical procedures 失效
    在诊断和分析程序中使用核酸类似物

    公开(公告)号：US06357163B1

    公开(公告)日：2002-03-19

    申请号：US08150156

    申请日：1994-05-04

    申请人： Ole Buchardt ,  Michael Egholm ,  Peter E. Nielsen ,  Rolf H. Berg

    发明人： Ole Buchardt ,  Michael Egholm ,  Peter E. Nielsen ,  Rolf H. Berg

    IPC分类号： C12Q168

    CPC分类号： C07H21/00 ,  A61K38/00 ,  C07K5/06026 ,  C07K5/06139 ,  C07K7/06 ,  C07K7/08 ,  C07K14/003 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6832 ,  C12Q1/6869 ,  C12Q2535/107 ,  C12Q2525/107

    摘要： Methods of capture, recognition, detection, identification or quantitation of nucleic acids and diagnostics uses generally are described in which are used: (a) a peptide nucleic acid (PNA) comprising a polyamide backbone bearing a plurality of ligands at respective spaced locations along said backbone, said ligands being each independently naturally occurring nucleobases, non-naturally occurring nucleobases or nucleobase-binding groups, each said ligand being bound directly or indirectly to a nitrogen atom in said backbone, and said ligand bearing nitrogen atoms mainly being separated from one another in said backbone by from 4 to 8 intervening atoms; or (b) a nucleic acid analogue capable of hybridizing to a nucleic acid of complementary sequence to form a hybrid which is more stable against denaturation by heat than a hybrid between the conventional deoxyribonucleotide corresponding to said analogue and said nucleic acid; or (c) a nucleic acid analogue capable of hybridizing to a double stranded nucleic acid in which one strand has a sequence complementary to said analogue, so as to displace the other strand from said one strand.

    摘要翻译： 通常描述了核酸和诊断用途的捕获，识别，检测，鉴定或定量的方法，其中使用：（a）肽核酸（PNA），其包含聚酰胺主链，所述聚酰胺主链在所述 所述配体各自独立地是天然存在的核碱基，非天然存在的核碱基或核碱基结合基团，每个所述配体直接或间接地结合到所述主链中的氮原子，并且所述配位体氮原子主要彼此分离 在所述骨架中通过4至8个插入的原子; 或（b）能够与互补序列核酸杂交的核酸类似物，以形成与对应于所述类似物的所述类似物和所述核酸的常规脱氧核糖核苷酸之间的杂交体相比，通过加热变性更稳定的杂交体; 或（c）能够与双链核酸杂交的核酸类似物，其中一条链具有与所述类似物互补的序列，以便从所述一条链取代另一条链。

    Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
    9.
    发明授权
    Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility 失效
    肽核酸

    公开(公告)号：US06710164B1

    公开(公告)日：2004-03-23

    申请号：US09230088

    申请日：1999-03-10

    申请人： Peter E. Nielsen ,  Michael Egholm ,  Rolf H. Berg ,  Ole Buchardt

    发明人： Peter E. Nielsen ,  Michael Egholm ,  Rolf H. Berg ,  Ole Buchardt

    IPC分类号： A61K3800

    CPC分类号： C07K5/06139 ,  C07K14/003 ,  C12Q1/68

    摘要： A novel class of compounds known as peptide nucleic acids, bind complementary DNA and RNA strands, and generally do so more strongly than the corresponding DNA or RNA strands while exhibiting increased sequence specificity and solubility. The peptide nucleic acids comprise ligands selected from a group consisting of naturally-occurring nucleobases and non-naturally-occurring nucleobases, including 2,6-diaminopurine, attached to a polyamide backbone, and contain alkyl amine side chains.

    摘要翻译： 被称为肽核酸的新一类化合物结合互补DNA和RNA链，并且通常比相应的DNA或RNA链更强烈地显示出增加的序列特异性和溶解性。 肽核酸包含选自天然存在的核碱基和非天然存在的核碱基的配体，包括连接到聚酰胺主链上的2,6-二氨基嘌呤，并且含有烷基胺侧链。