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公开(公告)号：US07375092B2
公开(公告)日：2008-05-20
申请号：US10302633
申请日：2002-11-22
CPC分类号： C12N15/8261 , A61K38/00 , C07K14/415 , C07K14/4738 , C07K2319/00 , C12N15/8263 , Y02A40/146 , Y10S977/911
摘要： The invention provides methods and compositions of selectively disrupting mitotic function in a target cell demonstrating undesirable mitotic function. Suitable target cells include mammalian, plant and bacterial cells, which cells may be in vitro or in situ. The general methods involve introducing into the target cell an effective amount of a peptide comprising contiguous acidic amino acids, such as Asp or Glu, whereby the undesirable mitotic function of the cell is selectively disrupted. In particular embodiments, the peptide comprises a Gm2S-1 peptide, particularly a lunasin and/or alisin peptide. The peptide may be introduced by transfecting the cell with a nucleic acid encoding the peptide.
摘要翻译： 本发明提供了选择性地破坏靶细胞中有丝分裂功能的方法和组合物，其显示出不期望的有丝分裂功能。 合适的靶细胞包括哺乳动物，植物和细菌细胞，哪些细胞可以在体外或原位。 一般方法包括将有效量的包含连续酸性氨基酸（例如Asp或Glu）的肽引入靶细胞，由此选择性地破坏细胞的不期望的有丝分裂功能。 在具体实施方案中，肽包含Gm2S-1肽，特别是lunasin和/或乳链菌肽。 可以通过用编码肽的核酸转染细胞来引入肽。
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公开(公告)号：US6107287A
公开(公告)日：2000-08-22
申请号：US938675
申请日：1997-09-25
CPC分类号： C12N15/8261 , C07K14/415 , C07K14/4738 , C12N15/8263 , A61K38/00 , C07K2319/00 , Y10S977/911
摘要： The invention provides methods and compositions of selectively disrupting mitotic function in a target cell demonstrating undesirable mitotic function. Suitable target cells include mammalian, plant and bacterial cells, which cells may be in vitro or in situ. The general methods involve introducing into the target cell an effective amount of a peptide comprising contiguous acidic amino acids, such as Asp or Glu, whereby the undesirable mitotic function of the cell is selectively disrupted. In particular embodiments, the peptide comprises a Gm2S-1 peptide, particularly a lunasin and/or alisin peptide. The peptide may be introduced by transfecting the cell with a nucleic acid encoding the peptide.
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公开(公告)号：US06544956B1
公开(公告)日：2003-04-08
申请号：US09531727
申请日：2000-03-21
IPC分类号： A61K4800
CPC分类号： C12N15/8261 , A61K38/00 , C07K14/415 , C07K14/4738 , C07K2319/00 , C12N15/8263 , Y02A40/146 , Y10S977/911
摘要： The invention provides methods and compositions of selectively disrupting mitotic function in a target cell demonstrating undesirable mitotic function. Suitable target cells include mammalian, plant and bacterial cells, which cells may be in vitro or in situ. The general methods involve introducing into the target cell an effective amount of a peptide comprising contiguous acidic amino acids, such as Asp or Glu, whereby the undesirable mitotic function of the cell is selectively disrupted. In particular embodiments, the peptide comprises a Gm2S-1 peptide, particularly a lunasin and/or alisin peptide. The peptide may be introduced by transfecting the cell with a nucleic acid encoding the peptide.
摘要翻译： 本发明提供了选择性地破坏靶细胞中有丝分裂功能的方法和组合物，其显示出不期望的有丝分裂功能。 合适的靶细胞包括哺乳动物，植物和细菌细胞，哪些细胞可以在体外或原位。 一般方法包括将有效量的包含连续酸性氨基酸（例如Asp或Glu）的肽引入靶细胞，由此选择性地破坏细胞的不期望的有丝分裂功能。 在具体实施方案中，肽包含Gm2S-1肽，特别是lunasin和/或乳链菌肽。 可以通过用编码肽的核酸转染细胞来引入肽。
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公开(公告)号：US06391848B1
公开(公告)日：2002-05-21
申请号：US09303814
申请日：1999-04-30
IPC分类号： A61K3800
摘要： The invention provides methods and comprisitions for delivering effective amounts of lunasin as a nutraceutical. The general formulation comprises a composition comprising an active unit dosage of a lunasin polypeptide and a pharmaceutically acceptable excipient. The formulations may be delivered or administered by oral ingestion, by topically contacting skin using well known techniques for dermal delivery, by introducing into a retained physiological fluids. The invention also provides methods for making the subject formulations by purifying lunasin polypeptides to the requisite purity, and combining said lunasin polypeptide with a pharmaceuitcally acceptible excipient in an orally active unit dosage. The lunasin source material may be soybeans, a recombinant lunasin polypeptide expression system, a synthetically produced lunasin, or extract or fraction thereof.
摘要翻译： 本发明提供了用于递送有效量的lunasin作为营养药物的方法和组合。 一般制剂包含包含活性单位剂量的lunasin多肽和药学上可接受的赋形剂的组合物。 制剂可以通过口服摄取，通过引入保留的生理液体，通过使用熟知的用于皮肤递送的技术将皮肤局部接触来递送或施用。 本发明还提供了通过将lunasin多肽纯化至所需纯度来制备本发明制剂的方法，并且以口服活性单位剂量将所述lunasin多肽与药物可接受的赋形剂组合。 黄芩素源材料可以是大豆，重组lunasin多肽表达系统，合成产生的lunasin或其提取物或其部分。
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公开(公告)号：US09678060B2
公开(公告)日：2017-06-13
申请号：US12682892
申请日：2008-10-10
IPC分类号： G01N33/50 , G01N33/574 , G01N33/68
CPC分类号： G01N33/5011 , G01N33/574 , G01N33/6875
摘要： Methods are provided for screening a test compound for anti-neoplastic activity by providing a test compound to a cancer cell and measuring the acetylation of Lysine 16 of the N-terminal tail of histone H4. Methods are also provided for using a test compound for staging a cancer tissue and for measuring the degree of the test compound's anti-neoplastic activity upon a cancer tissue by providing a test compound to a cancer tissue sample and measuring the degree of methylation of nucleotides within the 5′ region in the cancer tissue sample.
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公开(公告)号：US20100291044A1
公开(公告)日：2010-11-18
申请号：US12682892
申请日：2008-10-10
CPC分类号： G01N33/5011 , G01N33/574 , G01N33/6875
摘要： Methods are provided for screening a test compound for anti-neoplastic activity by providing a test compound to a cancer cell and measuring the acetylation of Lysine 16 of the N-terminal tail of histone H4. Methods are also provided for using a test compound for staging a cancer tissue and for measuring the degree of the test compound's anti-neoplastic activity upon a cancer tissue by providing a test compound to a cancer tissue sample and measuring the degree of methylation of nucleotides within the 5′ region in the cancer tissue sample.
摘要翻译： 提供了通过向癌细胞提供测试化合物并测量组蛋白H4的N末端尾的赖氨酸16的乙酰化来筛选测试化合物用于抗肿瘤活性的方法。 还提供了使用测试化合物来分期癌组织并测量化合物对癌组织的抗肿瘤活性的程度的方法，其通过向癌组织样品提供测试化合物并测量其中的核苷酸的甲基化程度 癌组织样本中的5'区域。
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