Isolation and composition of novel glycosidases
    9.
    发明授权
    Isolation and composition of novel glycosidases 失效
    新型糖苷酶的分离和组成

    公开(公告)号:US06358724B1

    公开(公告)日:2002-03-19

    申请号:US09883800

    申请日:2001-06-18

    CPC classification number: C12Y302/0105 C12N9/2471 C12Y302/01023

    Abstract: Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from Xanthomonas and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNac&bgr;1-X, Gal&agr;1-3R, Gal&agr;1-6R, Gal&bgr;1-3R, Fuc&agr;-2R, Fuc&agr;1-3R, Fuc&agr;1-4R, Man&agr;1-2R, Man&agr;1-3R, Man&agr;1-6R, Man&bgr;1-4R, Xyl&bgr;1-2R, Glc&bgr;1-4R, and Gal&bgr;1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.

    Abstract translation: 已经描述了能够切割选择的糖苷键的基本上纯的糖苷酶,其包括从黄单胞菌属和重组糖苷酶分离的糖苷酶。 已经鉴定了GlcNacbeta1-X,Galalpha1-3R,Galalpha1-6R,Galbeta1-3R,Fucalpha-2R,Fucalpha1-3R,Fucalpha1-4R,Manalpha1-2R,Manalpha1-3R,Manalpha1-6R,Manbeta1的分离的酶的底物特异性 -4R,Xylbeta1-2R,Glcbeta1-4R和Galbeta1-4R,其提供了选择性地切割碳水化合物底物中的糖苷键并形成修饰的碳水化合物的改善的能力。

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