公开(公告)号：US20110263022A1

公开(公告)日：2011-10-27

申请号：US13120375

申请日：2009-09-23

申请人： Bernd Krause ,  Markus Neubauer ,  Joachim Loercher

发明人： Bernd Krause ,  Markus Neubauer ,  Joachim Loercher

IPC分类号： C12N5/071 ,  C12M1/00 ,  C12N5/0775 ,  C12M3/00

CPC分类号： B01D67/009 ,  A61L2/022 ,  C12M25/02 ,  C12N5/0068 ,  C12N5/0663 ,  C12N2533/30 ,  C12N2533/52

摘要： A membrane for cultivating adherent or suspension cells, in particular adherent cells. The membrane permits adhesion and proliferation of the cells due to the irradiation of the wet or dry membrane with gamma or beta rays or an electron beam in a dose of from 12.5 to 175 kGy in the presence of oxygen. The resulting membrane may be used without any pre-treatment with surface-modifying substances. A method for preparing such an irradiated membrane for cultivating adherent or suspension cells. Methods of using such a membrane for cultivating adherent or suspension cells.

摘要翻译： 用于培养粘附或悬浮细胞，特别是贴壁细胞的膜。 由于在氧气存在下用γ射线或β射线或电子束以12.5至175kGy的剂量照射湿膜或干膜，所述膜允许细胞粘附和增殖。 所得到的膜可以在没有表面改性物质的预处理的情况下使用。 制备用于培养粘附或悬浮细胞的这种照射膜的方法。 使用这种膜来培养粘附或悬浮细胞的方法。

公开(公告)号：US10974201B2

公开(公告)日：2021-04-13

申请号：US13120375

申请日：2009-09-23

申请人： Bernd Krause ,  Markus Neubauer ,  Joachim Loercher

发明人： Bernd Krause ,  Markus Neubauer ,  Joachim Loercher

IPC分类号： C12M1/12 ,  B01D67/00 ,  C12N5/00 ,  C12N5/0775 ,  A61L2/02

摘要： A membrane for cultivating adherent or suspension cells, in particular adherent cells. The membrane permits adhesion and proliferation of the cells due to the irradiation of the wet or dry membrane with gamma or beta rays or an electron beam in a dose of from 12.5 to 175 kGy in the presence of oxygen. The resulting membrane may be used without any pre-treatment with surface-modifying substances. A method for preparing such an irradiated membrane for cultivating adherent or suspension cells. Methods of using such a membrane for cultivating adherent or suspension cells.