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1.发明授权Conjugate of a tissue non-specific alkaline phosphatase and dextran, process for its production and use thereof 失效组织非特异性碱性磷酸酶和葡聚糖的缀合物，其生产和使用的方法公开(公告)号：US06905689B2
公开(公告)日：2005-06-14
申请号：US10624154
申请日：2003-07-21
申请人： Bernd Schneidinger , Thomas Meier , Rainer Schmuck , Zhixin Shao
发明人： Bernd Schneidinger , Thomas Meier , Rainer Schmuck , Zhixin Shao
IPC分类号： G01N33/573 , C12N9/14 , C12N9/16 , C12N15/09 , C12Q1/42 , G01N33/96 , A61K39/385 , C07H21/04 , C07K14/00
摘要： A conjugate of a tissue non-specific alkaline phosphatase (tns-AP) and dextran which can be obtained by reacting unglycosylated tns-AP with activated dextran by incubation in aqueous solution, stopping the reaction and isolating the conjugate from the solution. The conjugate obtained in this manner is suitable as a standard for the determination of alkaline phosphatase.
摘要翻译： 组织非特异性碱性磷酸酶（tns-AP）和葡聚糖的缀合物，其可以通过在水溶液中温育而使未糖基化的tns-AP与活化的葡聚糖反应而获得，停止反应并从溶液中分离缀合物。 以这种方式获得的缀合物适合作为碱性磷酸酶测定的标准。
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公开(公告)号：US07517664B2
公开(公告)日：2009-04-14
申请号：US11559607
申请日：2006-11-14
CPC分类号： C12N9/0004 , C12N9/0006
摘要： The present invention relates to oxidoreductase apoenzyme variants which are enzymatically inactive but have coenzyme-binding properties. Further, the present invention relates to DNA sequences encoding these oxidoreductase apoenzyme variants, expression vectors containing such DNA sequences and the use of these oxidoreductase apoenzyme variants in diagnostic applications.
摘要翻译： 本发明涉及酶促无活性但具有辅酶结合特性的氧化还原酶脱辅基酶变体。 此外，本发明涉及编码这些氧化还原酶脱辅基酶变体的DNA序列，含有这些DNA序列的表达载体以及这些氧化还原酶脱辅基酶变体在诊断应用中的用途。
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公开(公告)号：US07172890B2
公开(公告)日：2007-02-06
申请号：US10975697
申请日：2004-10-28
CPC分类号： C12N9/0004 , C12N9/0006
摘要： The present invention relates to oxidoreductase apoenzyme variants which are enzymatically inactive but have coenzyme-binding properties. Further, the present invention relates to DNA sequences encoding these oxidoreductase apoenzyme variants, expression vectors containing such DNA sequences and the use of these oxidoreductase apoenzyme variants in diagnostic applications.
摘要翻译： 本发明涉及酶促无活性但具有辅酶结合特性的氧化还原酶脱辅基酶变体。 此外，本发明涉及编码这些氧化还原酶脱辅基酶变体的DNA序列，含有这些DNA序列的表达载体以及这些氧化还原酶脱辅基酶变体在诊断应用中的用途。
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公开(公告)号：US20070087398A1
公开(公告)日：2007-04-19
申请号：US11559607
申请日：2006-11-14
CPC分类号： C12N9/0004 , C12N9/0006
摘要： The present invention relates to oxidoreductase apoenzyme variants which are enzymaticaily inactive but have coenzyme-binding properties. Further, the present invention relates to DNA sequences encoding these oxidoreductase apoenzyme variants, expression vectors containing such DNA sequences and the use of these oxidoreductase apoenzyme variants in diagnostic applications.
摘要翻译： 本发明涉及酶促不饱和但具有辅酶结合性质的氧化还原酶脱辅基酶变体。 此外，本发明涉及编码这些氧化还原酶脱辅基酶变体的DNA序列，含有这些DNA序列的表达载体以及这些氧化还原酶脱辅基酶变体在诊断应用中的用途。
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公开(公告)号：US07132270B2
公开(公告)日：2006-11-07
申请号：US10319147
申请日：2002-12-13
申请人： Peter Kratzsch , Rainer Schmuck , Daniela Beck , Zhixin Shao , Detlef Thym , Wolfgang-Reinhold Knappe
发明人： Peter Kratzsch , Rainer Schmuck , Daniela Beck , Zhixin Shao , Detlef Thym , Wolfgang-Reinhold Knappe
IPC分类号： C12N9/04
CPC分类号： C12N9/0006
摘要： The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.
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公开(公告)号：US06958231B2
公开(公告)日：2005-10-25
申请号：US10251078
申请日：2002-09-20
申请人： Zhixin Shao , Rainer Schmuck , Peter Kratzsch , Janet Kenklies , Harald Weisser
发明人： Zhixin Shao , Rainer Schmuck , Peter Kratzsch , Janet Kenklies , Harald Weisser
IPC分类号： C12N15/09 , C12N1/21 , C12N9/78 , C12Q1/26 , C12Q1/28 , C12Q1/34 , C12R1/18 , C07H21/04 , C12N9/02
CPC分类号： C12N9/78
摘要： The present invention relates to mutated genes encoding variants of an Erwinia-type creatinase (EC 3.5.3.3, alternative name: creatine amidinohydrolase), to the creatinase variants encoded by these genes, and to different applications of these variants of creatinase, particularly to their use for determining the creatinine and/or creatine concentration in a sample.
摘要翻译： 本发明涉及编码由这些基因编码的肌酸酐酶变体的欧文氏菌型肌酸酐酶（EC 3.5.3.3，替代名称：肌酸脒基水解酶）的变体的突变基因，以及这些肌酸酐酶变体的不同应用，特别是涉及它们 用于测定样品中肌酐和/或肌酸浓度。
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7.发明授权公开(公告)号：US07547535B2
公开(公告)日：2009-06-16
申请号：US11298778
申请日：2005-12-09
申请人： Peter Kratzsch , Rainer Schmuck , Daniela Beck , Zhixin Shao , Detlef Thym , Wolfgang-Reinhold Knappe
发明人： Peter Kratzsch , Rainer Schmuck , Daniela Beck , Zhixin Shao , Detlef Thym , Wolfgang-Reinhold Knappe
CPC分类号： C12N9/0006
摘要： The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.
摘要翻译： 本发明涉及可溶性吡咯并喹啉醌（PQQ） - 依赖性葡萄糖脱氢酶（s-GDH），编码突变s-GDH的基因与s-GDH突变体蛋白质的改进变体，其具有改善的对葡萄糖的底物特异性以及不同应用 的这些s-GDH变体，特别是用于测定样品中糖的浓度，特别是葡萄糖的浓度。
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公开(公告)号：US20060148056A1
公开(公告)日：2006-07-06
申请号：US11298778
申请日：2005-12-09
申请人： Peter Kratzsch , Rainer Schmuck , Daniela Beck , Zhixin Shao , Detlef Thym , Wolfgang-Reinhold Knappe
发明人： Peter Kratzsch , Rainer Schmuck , Daniela Beck , Zhixin Shao , Detlef Thym , Wolfgang-Reinhold Knappe
IPC分类号： C12N9/02
CPC分类号： C12N9/0006
摘要： The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.
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公开(公告)号：US20060094099A1
公开(公告)日：2006-05-04
申请号：US10975697
申请日：2004-10-28
CPC分类号： C12N9/0004 , C12N9/0006
摘要： The present invention relates to oxidoreductase apoenzyme variants which are enzymatically inactive but have coenzyme-binding properties. Further, the present invention relates to DNA sequences encoding these oxidoreductase apoenzyme variants, expression vectors containing such DNA sequences and the use of these oxidoreductase apoenzyme variants in diagnostic applications.
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公开(公告)号：US06361988B1
公开(公告)日：2002-03-26
申请号：US09543084
申请日：2000-04-04
申请人： Frances H. Arnold , Zhixin Shao , Huimin Zhao , Lorraine J. Giver
发明人： Frances H. Arnold , Zhixin Shao , Huimin Zhao , Lorraine J. Giver
IPC分类号： C12N916
CPC分类号： C12N9/80
摘要： A method for in vitro mutagenesis and recombination of polynucleotide sequences based on polymerase-catalyzed extension of primer oligonucleotides is disclosed. The method involves priming template polynucleotide(s) with random-sequences or defined-sequence primers to generate a pool of short DNA fragments with a low level of point mutations. The DNA fragments are subjected to denaturization followed by annealing and further enzyme-catalyzed DNA polymerization. This procedure is repeated a sufficient number of times to produce full-length genes which comprise mutants of the original template polynucleotides. These genes can be further amplified by the polymerase chain reaction and cloned into a vector for expression of the encoded proteins.
摘要翻译： 公开了一种基于聚合酶催化的引物寡核苷酸延伸的多核苷酸序列的体外诱变和重组方法。 该方法包括用随机序列或定义序列引物引发模板多核苷酸以产生具有低水平点突变的短DNA片段池。 将DNA片段进行变性，然后退火并进一步进行酶催化的DNA聚合。 重复该过程足够次数以产生包含原始模板多核苷酸突变体的全长基因。 这些基因可以通过聚合酶链反应进一步扩增，并克隆到用于表达编码蛋白质的载体中。
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