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1.发明授权Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity 有权用标记的探针和3'至5'核酸外切酶活性进行定量扩增公开(公告)号：US08426132B2
公开(公告)日：2013-04-23
申请号：US12264200
申请日：2008-11-03
申请人： Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
发明人： Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
CPC分类号： C12Q1/6851 , C12Q2561/101 , C12Q2525/161 , C12Q2521/101 , C12Q2537/143 , C12Q2527/143
摘要： This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3′ to 5′ exonuclease activity that cleaves the 3′ oligonucleotide of the probe.
摘要翻译： 本发明提供了进行定量扩增反应的方法和试剂盒。 该方法采用聚合酶和具有切割探针的3'寡核苷酸的3'至5'核酸外切酶活性的酶。
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2.发明授权Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity 有权用标记的探针和3'至5'核酸外切酶活性进行定量扩增公开(公告)号：US07445898B2
公开(公告)日：2008-11-04
申请号：US11097463
申请日：2005-03-31
申请人： Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
发明人： Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
CPC分类号： C12Q1/6851 , C12Q2561/101 , C12Q2525/161 , C12Q2521/101 , C12Q2537/143 , C12Q2527/143
摘要： This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3′ to 5′ exonuclease activity that cleaves the 3′ oligonucleotide of the probe.
摘要翻译： 本发明提供了进行定量扩增反应的方法和试剂盒。 该方法采用聚合酶和具有切割探针的3'寡核苷酸的3'至5'核酸外切酶活性的酶。
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3.发明申请QUANTITATIVE AMPLIFICATION WITH A LABELED PROBE AND 3' TO 5' EXONUCLEASE ACTIVITY 有权具有标记探针和3'至5'外切酶活性的定量放大公开(公告)号：US20100159447A1
公开(公告)日：2010-06-24
申请号：US12264200
申请日：2008-11-03
申请人： Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
发明人： Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2561/101 , C12Q2525/161 , C12Q2521/101 , C12Q2537/143 , C12Q2527/143
摘要： This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3′ to 5′ exonuclease activity that cleaves the 3′ oligonucleotide of the probe.
摘要翻译： 本发明提供了进行定量扩增反应的方法和试剂盒。 该方法采用聚合酶和具有切割探针的3'寡核苷酸的3'至5'核酸外切酶活性的酶。
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公开(公告)号：US20060024695A1
公开(公告)日：2006-02-02
申请号：US11097463
申请日：2005-03-31
申请人： Bin Li , Lei Xi , Yan Wang , Peter Vander Horn
发明人： Bin Li , Lei Xi , Yan Wang , Peter Vander Horn
CPC分类号： C12Q1/6851 , C12Q2561/101 , C12Q2525/161 , C12Q2521/101 , C12Q2537/143 , C12Q2527/143
摘要： This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3′ to 5′ exonuclease activity that cleaves the 3′ oligonucleotide of the probe.
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公开(公告)号：US07560260B2
公开(公告)日：2009-07-14
申请号：US10627582
申请日：2003-07-25
申请人： Peter B. Vander Horn , Yan Wang
发明人： Peter B. Vander Horn , Yan Wang
CPC分类号： C12N9/1252 , C07K2319/00 , C12N9/1241 , C12Y207/07007
摘要： The invention provides novel compositions with polymerase activity and methods of using the compositions.
摘要翻译： 本发明提供具有聚合酶活性的新型组合物和使用该组合物的方法。
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公开(公告)号：US08367376B2
公开(公告)日：2013-02-05
申请号：US12494217
申请日：2009-06-29
申请人： Peter B. Vander Horn , Yan Wang
发明人： Peter B. Vander Horn , Yan Wang
CPC分类号： C12N9/1252 , C07K2319/00 , C12N9/1241 , C12Y207/07007
摘要： The invention provides novel compositions with polymerase activity and methods of using the compositions.
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公开(公告)号：US20090269829A1
公开(公告)日：2009-10-29
申请号：US12494217
申请日：2009-06-29
申请人： Peter B. Vander Horn , Yan Wang
发明人： Peter B. Vander Horn , Yan Wang
CPC分类号： C12N9/1252 , C07K2319/00 , C12N9/1241 , C12Y207/07007
摘要： The invention provides novel compositions with polymerase activity and methods of using the compositions.
摘要翻译： 本发明提供具有聚合酶活性的新型组合物和使用该组合物的方法。
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公开(公告)号：US08476045B2
公开(公告)日：2013-07-02
申请号：US13560075
申请日：2012-07-27
申请人： Yan Wang , Peter Vander Horn , Lei Xi
发明人： Yan Wang , Peter Vander Horn , Lei Xi
IPC分类号： C12P19/34
CPC分类号： C12N15/10 , C07K14/195 , C07K2319/00 , C07K2319/80 , C12N9/1252 , C12N15/62 , C12P19/34 , C12Q1/686 , C12Y207/07007
摘要： This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.
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公开(公告)号：US07670808B2
公开(公告)日：2010-03-02
申请号：US10821583
申请日：2004-04-09
申请人： Yan Wang , Lei Xi , Dennis E. Prosen
发明人： Yan Wang , Lei Xi , Dennis E. Prosen
CPC分类号： C12N9/1276 , C07K14/00 , C07K14/001 , C07K19/00 , C07K2319/80 , C12N9/1252 , C12N9/96 , C12P19/34 , C12Y207/07007 , C12Y207/07049 , Y02P20/52
摘要： This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
摘要翻译： 本发明提供了改进的新型核酸修饰酶的产生。 改进是以提高酶结合和催化修饰核酸的能力的方式将序列非特异性核酸结合结构域与酶的融合。
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公开(公告)号：US07541170B2
公开(公告)日：2009-06-02
申请号：US09870353
申请日：2001-05-30
申请人： Yan Wang , Lei Xi , Dennis E. Prosen
发明人： Yan Wang , Lei Xi , Dennis E. Prosen
CPC分类号： C12N9/1276 , C07K14/00 , C07K14/001 , C07K19/00 , C07K2319/80 , C12N9/1252 , C12N9/96 , C12P19/34 , C12Y207/07007 , C12Y207/07049 , Y02P20/52
摘要： This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
摘要翻译： 本发明提供了改进的新型核酸修饰酶的产生。 改进是以提高酶结合和催化修饰核酸的能力的方式将序列非特异性核酸结合结构域与酶的融合。
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