公开(公告)号：US20230070234A1

公开(公告)日：2023-03-09

申请号：US17986873

申请日：2022-11-14

申请人： Bio-Rad Laboratories, Inc.

发明人： David P. STUMBO ,  George CARMAN ,  Steve HOBBS ,  Anthony J. MAKAREWICZ, JR. ,  Dmitri SIMONIAN ,  David GLADE ,  Joshua OEN ,  Denis PRISTINSKI ,  John DZENITIS

IPC分类号： C12Q1/686 ,  B01L3/00 ,  G01N15/14 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6851 ,  B01F23/41

摘要： Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：US20190002956A1

公开(公告)日：2019-01-03

申请号：US16022500

申请日：2018-06-28

申请人： Bio-Rad Laboratories, Inc.

发明人： David P. STUMBO ,  George CARMAN ,  Steve HOBBS ,  Anthony J. MAKAREWICZ, JR. ,  Dmitri SIMONIAN ,  David GLADE ,  Joshua OEN ,  Denis PRISTINSKI ,  John DZENITIS

IPC分类号： C12Q1/686 ,  B01L3/00 ,  G01N15/14 ,  B01F3/08 ,  C12Q1/6848 ,  C12Q1/6844

摘要： Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.