公开(公告)号：US20210283608A1

公开(公告)日：2021-09-16

申请号：US17333142

申请日：2021-05-28

申请人： Bio-Rad Laboratories, Inc.

发明人： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC分类号： B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14

摘要： A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

公开(公告)号：US20220152606A1

公开(公告)日：2022-05-19

申请号：US17590543

申请日：2022-02-01

申请人： Bio-Rad Laboratories, Inc.

发明人： Kalyan Handique ,  Swati Ranade ,  Vishal Sharma ,  Austin Payne ,  Sam Tuck

IPC分类号： B01L3/00 ,  C12N13/00

摘要： A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).

公开(公告)号：US11273439B2

公开(公告)日：2022-03-15

申请号：US16906337

申请日：2020-06-19

申请人： Bio-Rad Laboratories, Inc

发明人： Kalyan Handique ,  Swati Ranade ,  Vishal Sharma ,  Austin Payne ,  Sam Tuck

IPC分类号： B01L3/00 ,  C12N13/00

摘要： A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).

公开(公告)号：US11504714B2

公开(公告)日：2022-11-22

申请号：US16790583

申请日：2020-02-13

申请人： Bio-Rad Laboratories, Inc.

发明人： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC分类号： G01N33/543 ,  B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/569 ,  G01N15/14 ,  C12Q1/6886 ,  G01N15/10

摘要： A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

公开(公告)号：US20240066519A1

公开(公告)日：2024-02-29

申请号：US18381818

申请日：2023-10-19

申请人： Bio-Rad Laboratories, Inc.

发明人： Kalyan Handique ,  Swati Ranade ,  Vishal Sharma ,  Austin Payne ,  Sam Tuck

IPC分类号： B01L3/00 ,  C12N13/00

CPC分类号： B01L3/502 ,  C12N13/00 ,  B01L2200/026 ,  B01L2300/0893 ,  B01L2400/043

摘要： A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).

公开(公告)号：US20230037836A1

公开(公告)日：2023-02-09

申请号：US17965495

申请日：2022-10-13

申请人： Bio-Rad Laboratories, Inc.

发明人： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC分类号： B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14

摘要： A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

公开(公告)号：US20240253038A1

公开(公告)日：2024-08-01

申请号：US18233819

申请日：2023-08-14

申请人： Bio-Rad Laboratories, Inc.

发明人： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC分类号： B01L3/00 ,  C12M1/00 ,  C12Q1/6816 ,  C12Q1/6886 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1434 ,  G01N15/149 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574

CPC分类号： B01L3/502761 ,  C12M47/04 ,  C12Q1/6816 ,  G01N15/1434 ,  G01N15/1484 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574 ,  B01L3/502715 ,  B01L3/527 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2400/0406 ,  C12Q1/6886 ,  G01N2015/1006 ,  G01N15/149

摘要： A system and method, wherein the method includes: (a) receiving a fluid comprising a process reagent for an assay and nucleic acid material from a sample into a fluid pathway coupled to an array of wells, wherein the array of wells is defined within a substrate and each well in the array of wells extends vertically into a planar surface of the substrate; (b) distributing the fluid along the fluid pathway and into wells of the array of wells; and (c) distributing a liquid immiscible with the fluid comprising the process reagent and nucleic acid material along the fluid pathway upon positive pressure generation by a pump of a flow control system in communication with an inlet to the fluid pathway, thereby displacing said fluid along the fluid pathway and into wells of the array of wells and preventing contents of each well from transferring to adjacent wells.

公开(公告)号：US11865542B2

公开(公告)日：2024-01-09

申请号：US17965495

申请日：2022-10-13

申请人： Bio-Rad Laboratories, Inc.

发明人： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC分类号： B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14 ,  C12Q1/6886 ,  G01N15/10

CPC分类号： B01L3/502761 ,  C12M47/04 ,  C12Q1/6816 ,  G01N15/1434 ,  G01N15/1484 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574 ,  B01L3/502715 ,  B01L3/527 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2400/0406 ,  C12Q1/6886 ,  G01N2015/1006 ,  G01N2015/149

摘要： A system and method, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity and a fluid delivery module including a fluid pathway through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: generating a set of genetic complexes within individual wells of the array of wells.

公开(公告)号：US11833507B2

公开(公告)日：2023-12-05

申请号：US17590543

申请日：2022-02-01

申请人： Bio-Rad Laboratories, Inc.

发明人： Kalyan Handique ,  Swati Ranade ,  Vishal Sharma ,  Austin Payne ,  Sam Tuck

IPC分类号： B01L3/00 ,  C12N13/00

CPC分类号： B01L3/502 ,  C12N13/00 ,  B01L2200/026 ,  B01L2300/0893 ,  B01L2400/043

摘要： A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).

公开(公告)号：US11358146B2

公开(公告)日：2022-06-14

申请号：US17333142

申请日：2021-05-28

申请人： Bio-Rad Laboratories, Inc.

发明人： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC分类号： C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  C12Q1/6886 ,  G01N15/10 ,  B01L3/00 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14

摘要： A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir through which fluid flow is controlled along a fluid path; and wherein the method includes: capturing a population of particles into the array of wells in single-particle format; releasing, from the particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.