公开(公告)号：US11578322B2

公开(公告)日：2023-02-14

申请号：US16867256

申请日：2020-05-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Sida Wang ,  Patrick Michael Tegels ,  William T. Koederitz ,  Daniel Genord ,  Grey Parker ,  Brian Boniface ,  Katlyn Curtin Mehne ,  Alec William Hitchiner

IPC: C12Q1/68 ,  C12N15/10 ,  B01L3/00 ,  B01L7/00 ,  G01F23/292 ,  C12N1/06 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844

Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.

公开(公告)号：US20230159917A1

公开(公告)日：2023-05-25

申请号：US18094278

申请日：2023-01-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Sida Wang ,  Patrick Michael Tegels ,  William T. Koederitz ,  Daniel Genord ,  Grey Parker ,  Brian Boniface ,  Katlyn Curtin Mehne ,  Alec William Hitchiner

IPC: C12N15/10 ,  B01L3/00 ,  B01L7/00 ,  G01F23/292 ,  C12N1/06 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844

CPC classification number: C12N15/1096 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  C12N15/1093 ,  G01F23/292 ,  C12N1/06 ,  B01L3/502 ,  B01L3/52 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844 ,  B01L2400/06 ,  B01L2300/123 ,  B01L2200/0647 ,  B01L2300/041 ,  B01L2300/0829 ,  B01L2300/0819 ,  B01L2300/1805 ,  B01L2300/047 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/042 ,  B01L2300/0627 ,  B01L2300/0893 ,  B01L2300/1894 ,  B01L2400/043 ,  B01L2400/0633 ,  B01L2300/16

Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.