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    Optimization of gene expression analysis using immobilized capture probes
    1.
    发明授权
    Optimization of gene expression analysis using immobilized capture probes 有权

    公开(公告)号：US10407718B2

    公开(公告)日：2019-09-10

    申请号：US15491127

    申请日：2017-04-19

    Applicant: BioArray Solutions, Ltd.

    Inventor： Michael Seul ,  Sukanta Banerjee ,  Jiacheng Yang ,  Tatiana Vener

    IPC: C12Q1/6832 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6837

    Abstract: Disclosed are methods of multiplexed analysis of oligonucleotides in a sample, including a method of preventing a significant reduction in duplexes detectable in a hybridization assay involving (i) selecting probe lengths for sets of oligonucleotide probes, wherein probes include different subsequences such that at least one subsequence is complementary to a subsequence in a cognate target; wherein probes for longer cognate targets are longer in length than probes for shorter cognate targets, (ii) selecting, for each set of probes, a density of oligonucleotides probes attached per unit area on a solid phase carrier which is below a limit at which the significant reduction in detectable duplexes is predicated to take place, (iii) producing the probes and affixing them to different solid phase carriers at the selected density, and (iv) annealing targets to the probes, wherein signal intensities of probes and targets of different lengths are about the same.

    Optimization Of Gene Expression Analysis Using Immobilized Capture Probes
    10.
    发明申请
    Optimization Of Gene Expression Analysis Using Immobilized Capture Probes 有权
    使用固定化捕获探针优化基因表达分析

    公开(公告)号：US20160215327A1

    公开(公告)日：2016-07-28

    申请号：US14257294

    申请日：2014-04-21

    Applicant: BIOARRAY SOLUTIONS, LTD.

    Inventor： Michael Seul ,  Sukanta Banerjee ,  Jiacheng Yang ,  Tatiana Vener

    IPC: C12Q1/68

    CPC classification number: C12Q1/6832 ,  B01J2219/00459 ,  B01J2219/00576 ,  B01J2219/00608 ,  B01J2219/00648 ,  B01J2219/00722 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2537/143 ,  C12Q2565/501 ,  C12Q2565/507 ,  C12Q2533/101 ,  C12Q2565/514 ,  C12Q2565/519

    Abstract: Disclosed are methods of multiplexed analysis of oligonucleotides in a sample, including a method of preventing a significant reduction in duplexes detectable in a hybridization assay involving (i) selecting probe lengths for sets of oligonucleotide probes, wherein probes include different subsequences such that at least one subsequence is complementary to a subsequence in a cognate target; wherein probes for longer cognate targets are longer in length than probes for shorter cognate targets, (ii) selecting, for each set of probes, a density of oligonucleotides probes attached per unit area on a solid phase carrier which is below a limit at which the significant reduction in detectable duplexes is predicated to take place, (iii) producing the probes and affixing them to different solid phase carriers at the selected density, and (iv) annealing targets to the probes, wherein signal intensities of probes and targets of different lengths are about the same.

    Abstract translation: 公开了一种样品中寡核苷酸多重分析的方法，包括防止在杂交测定中可检测到的双链体显着降低的方法，其包括（i）选择寡核苷酸探针组的探针长度，其中探针包括不同的子序列，使得至少一个 子序列是同源目标中的一个子序列的补充; 其中用于较长同源靶的探针的长度长于针对较短同源靶的探针，（ii）为每组探针选择在固相载体上每单位面积连接的寡核苷酸探针的密度，其低于 预测发现可检测的双链体显着减少，（iii）产生探针并以选定的密度将它们固定在不同的固相载体上，和（iv）将靶标退火至探针，其中不同长度的探针和靶的信号强度 大致相同。

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