公开(公告)号：US20190002579A1

公开(公告)日：2019-01-03

申请号：US15961968

申请日：2018-04-25

申请人： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

发明人： George GEORGIOU ,  Everett STONE ,  Nicholas MARSHALL ,  John BLAZECK ,  Wei-Cheng LU

IPC分类号： C07K16/28 ,  C12N9/96 ,  C12N9/14 ,  A61K39/395 ,  A61K47/60 ,  A61K35/17 ,  A61K38/00

摘要： Methods and compositions related to the use of a protein with kynureninase activity are described. For example, in certain aspects there may be disclosed a modified kynureninase capable of degrading kynurenine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with kynurenine depletion using the disclosed proteins or nucleic acids.

公开(公告)号：US20150064159A1

公开(公告)日：2015-03-05

申请号：US14472750

申请日：2014-08-29

申请人： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

发明人： George GEORGIOU ,  Everett STONE ,  Wei-Cheng LU

IPC分类号： C12N9/88 ,  A61K45/06 ,  A61K38/51

CPC分类号： C12N9/88 ,  A61K35/12 ,  A61K38/51 ,  A61K45/06 ,  C12N1/14 ,  C12N1/20 ,  C12N5/0601 ,  C12N15/70 ,  C12N15/74 ,  C12N15/79 ,  C12Y404/01001 ,  A61K2300/00

摘要： Methods and compositions relating to the engineering of an improved protein with methionine-γ-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-γ-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids.

摘要翻译： 描述了与具有甲硫氨酸-γ-裂解酶活性的改进蛋白质的工程相关的方法和组合物。 例如，在某些方面，可以公开包含一个或多个氨基酸取代并能降解甲硫氨酸的修饰的胱硫醚-γ-裂解酶（CGL）。 此外，本发明的某些方面提供使用所公开的蛋白质或核酸用于用甲硫氨酸消耗治疗癌症的组合物和方法。

公开(公告)号：US20210380962A1

公开(公告)日：2021-12-09

申请号：US17288151

申请日：2019-10-25

申请人： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

发明人： Everett STONE ,  Wei-Cheng LU ,  Christos KARAMITROS

IPC分类号： C12N9/88 ,  A61B17/225 ,  A61K45/06 ,  A61K47/60

摘要： Methods and compositions related to the engineering of a protein with L-cyst(e)ine degrading enzyme activity are described. For example, disclosed are modified cystathionine-γ-lyases comprising one or more amino acid substitutions and capable of degrading L-cyst(e)ine. Furthermore, compositions and methods are provided for the treatment of cystinuria using the disclosed modified enzymes or nucleic acids encoding said enzymes.

公开(公告)号：US20170044514A1

公开(公告)日：2017-02-16

申请号：US15297102

申请日：2016-10-18

申请人： Board of Regents, The University of Texas System

发明人： George GEORGIOU ,  Everett STONE ,  Wei-Cheng LU

IPC分类号： C12N9/88

CPC分类号： C12N9/88 ,  A61K35/12 ,  A61K38/51 ,  A61K45/06 ,  C12N1/14 ,  C12N1/20 ,  C12N5/0601 ,  C12N15/70 ,  C12N15/74 ,  C12N15/79 ,  C12Y404/01001 ,  A61K2300/00

摘要： Methods and compositions relating to the engineering of an improved protein with methionine-γ-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-γ-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids.

摘要翻译： 描述了与具有甲硫氨酸-γ-裂解酶活性的改进蛋白质的工程相关的方法和组合物。 例如，在某些方面，可以公开包含一个或多个氨基酸取代并能降解甲硫氨酸的修饰的胱硫醚-γ-裂解酶（CGL）。 此外，本发明的某些方面提供使用所公开的蛋白质或核酸用于用甲硫氨酸消耗治疗癌症的组合物和方法。

公开(公告)号：US20220275099A1

公开(公告)日：2022-09-01

申请号：US17498520

申请日：2021-10-11

申请人： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

发明人： George GEORGIOU ,  Everett STONE ,  Nicholas MARSHALL ,  John BLAZECK ,  Wei-Cheng LU

IPC分类号： C07K16/28 ,  C12N9/14 ,  C12N9/96 ,  A61K39/395 ,  A61K47/60

摘要： Methods and compositions related to the use of a protein with kynureninase activity are described. For example, in certain aspects there may be disclosed a modified kynureninase capable of degrading kynurenine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with kynurenine depletion using the disclosed proteins or nucleic acids.

公开(公告)号：US20210386838A1

公开(公告)日：2021-12-16

申请号：US17330106

申请日：2021-05-25

申请人： Board of Regents, The University of Texas System

发明人： George GEORGIOU ,  Everett STONE ,  Wei-Cheng LU

IPC分类号： A61K38/51 ,  A61K47/60 ,  C12N9/96 ,  A61K9/00 ,  A61P7/00 ,  C12N15/70 ,  C12N15/861 ,  C12N15/869 ,  C12N15/867 ,  C12N15/80 ,  C12N9/88 ,  A61K9/19

摘要： Methods and compositions relating to the engineering of an improved protein with homocyst(e)inase enzyme activity are described. For example, there are disclosed modified cystathionine-γ-lyase (CGL) enzymes comprising one or more amino acid substitutions and capable of degrading homocyst(e)ine. Furthermore, provided are compositions and methods for the treatment of homocystinuria or hyperhomocysteinemia with homocyst(e)ine depletion using the disclosed enzymes or nucleic acids.

公开(公告)号：US20180326025A1

公开(公告)日：2018-11-15

申请号：US15977299

申请日：2018-05-11

申请人： Board of Regents, The University of Texas System

发明人： George GEORGIOU ,  Everett STONE ,  Wei-Cheng LU

IPC分类号： A61K38/51 ,  A61K47/60 ,  C12N9/96 ,  A61K9/00 ,  A61P7/00 ,  C12N15/70 ,  C12N15/80 ,  C12N15/861 ,  C12N15/869 ,  C12N15/867

CPC分类号： A61K38/51 ,  A61K9/0019 ,  A61K31/198 ,  A61K31/4415 ,  A61K38/00 ,  A61K47/60 ,  A61P7/00 ,  C12N9/88 ,  C12N9/96 ,  C12N15/70 ,  C12N15/80 ,  C12N15/861 ,  C12N15/867 ,  C12N15/869 ,  C12Y404/01001

摘要： Methods and compositions relating to the engineering of an improved protein with homocyst(e)inase enzyme activity are described. For example, there are disclosed modified cystathionine-γ-lyase (CGL) enzymes comprising one or more amino acid substitutions and capable of degrading homocyst(e)ine. Furthermore, provided are compositions and methods for the treatment of homocystinuria or hyperhomocysteinemia with homocyst(e)ine depletion using the disclosed enzymes or nucleic acids.

公开(公告)号：US20180002685A1

公开(公告)日：2018-01-04

申请号：US15451349

申请日：2017-03-06

申请人： Board of Regents, The University of Texas System

发明人： George GEORGIOU ,  Everett STONE ,  Wei-Cheng LU

IPC分类号： C12N9/88 ,  C12N15/79 ,  C12N15/74 ,  A61K38/51 ,  A61K35/12 ,  C12N1/20 ,  C12N1/14 ,  A61K45/06 ,  C12N5/07 ,  C12N15/70

CPC分类号： C12N9/88 ,  A61K35/12 ,  A61K38/51 ,  A61K45/06 ,  C12N1/14 ,  C12N1/20 ,  C12N5/0601 ,  C12N15/70 ,  C12N15/74 ,  C12N15/79 ,  C12Y404/01001 ,  A61K2300/00

摘要： Methods and compositions relating to the engineering of an improved protein with methionine-γ-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-γ-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids.

公开(公告)号：US20160058845A1

公开(公告)日：2016-03-03

申请号：US14839293

申请日：2015-08-28

申请人： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

发明人： George GEORGIOU ,  Everett STONE ,  Nicholas MARSHALL ,  John BLAZECK ,  Wei-Cheng LU

IPC分类号： A61K38/46 ,  C12N9/14 ,  C07K16/28 ,  A61K47/48 ,  C07K14/725 ,  C07K14/705 ,  A61K35/17 ,  C12N9/96

CPC分类号： C07K16/2887 ,  A61K35/17 ,  A61K38/00 ,  A61K39/3955 ,  A61K47/60 ,  C07K16/2818 ,  C07K16/2827 ,  C07K16/2896 ,  C07K2317/622 ,  C07K2319/01 ,  C12N9/14 ,  C12N9/96 ,  C12Y307/01003

摘要： Methods and compositions related to the use of a protein with kynureninase activity are described. For example, in certain aspects there may be disclosed a modified kynureninase capable of degrading kynurenine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with kynurenine depletion using the disclosed proteins or nucleic acids.