公开(公告)号：US08758822B2

公开(公告)日：2014-06-24

申请号：US12990540

申请日：2009-04-30

申请人： Bong Hyun Chung ,  Yong Taik Lim ,  Jung Hyun Han

发明人： Bong Hyun Chung ,  Yong Taik Lim ,  Jung Hyun Han

IPC分类号： A61K9/14 ,  A61K38/00 ,  A61K38/36 ,  A61K38/13 ,  A01N45/00 ,  A61K31/56 ,  A61K31/585 ,  A61K31/54 ,  A61K31/505 ,  A61K31/095 ,  A61K31/045 ,  B01D61/00 ,  A61K9/16 ,  C08J3/03 ,  B01D65/00

CPC分类号： A61K9/1652 ,  A61K9/5153 ,  A61K31/235 ,  A61K31/343 ,  A61K31/513 ,  A61K31/519 ,  A61K31/5415 ,  A61K31/573 ,  A61K31/585 ,  A61K31/704 ,  A61K31/7048 ,  B01D65/00 ,  C08J3/03 ,  Y10S516/929

摘要： The present invention relates to a method for manufacturing uniform size polymeric nanoparticles containing poorly soluble drugs, and more particularly, to a method for manufacturing uniform size polymeric nanoparticles containing poorly soluble drugs, including a first step of dissolving a biodegradable polymer in a non-volatile polar organic solvent, a second step of adding poorly soluble drugs to water and the biodegradable polymer solution to produce a dispersion, and a third step of adding the dispersion to emulsifier solutions in a batch under the condition of low mechanical energy level stirring. The polymeric nanoparticles of the present invention is capable of manufacturing nano-sized small and uniform polymeric nanoparticles through a simple method of employing a non-volatile polar solvent, especially a solvent having a polarity similar to that of water, as a solvent for a mixture solution of biodegradable polymer and poorly soluble materials, and using a low mechanical energy condition and batch-adding of dispersion in an emulsifying process. The polymeric nanoparticles of the present invention are advantageous in that the dissolution rate of the poorly soluble drugs contained in polymeric particles is dramatically improved, and the poorly soluble drugs are gradually and steadily released and maintained at a constant density over a long period of time.

摘要翻译： 本发明涉及一种制备均匀尺寸的含有难溶性药物的聚合物纳米颗粒的方法，更具体地说，涉及一种制备均匀尺寸的含有难溶性药物的聚合物纳米颗粒的方法，包括将可生物降解聚合物溶解在非挥发性物质中的第一步骤 极性有机溶剂，向水中添加难溶性药物和生物降解性聚合物溶液以产生分散体的第二步骤，以及在低机械能级搅拌的条件下将分散体分批加入到乳化剂溶液中的第三步骤。 本发明的聚合物纳米颗粒能够通过使用非挥发性极性溶剂，特别是极性类似于水的溶剂的简单方法作为混合物的溶剂来制造纳米尺寸的小而均匀的聚合物纳米颗粒 可生物降解聚合物和难溶性物质的溶液，并且使用低机械能条件和在乳化过程中分批添加分散体。 本发明的聚合物纳米颗粒的优点在于，包含在聚合物颗粒中的难溶性药物的溶解速度显着提高，并且难溶性药物在长时间内逐渐稳定地释放并保持在恒定的密度。

公开(公告)号：US20110171269A1

公开(公告)日：2011-07-14

申请号：US12990540

申请日：2009-04-30

申请人： Bong Hyun Chung ,  Yong Taik Lim ,  Jung Hyun Han

发明人： Bong Hyun Chung ,  Yong Taik Lim ,  Jung Hyun Han

IPC分类号： A61K9/00 ,  A61K31/235 ,  A61P1/16 ,  B82Y40/00 ,  B82Y5/00

CPC分类号： A61K9/1652 ,  A61K9/5153 ,  A61K31/235 ,  A61K31/343 ,  A61K31/513 ,  A61K31/519 ,  A61K31/5415 ,  A61K31/573 ,  A61K31/585 ,  A61K31/704 ,  A61K31/7048 ,  B01D65/00 ,  C08J3/03 ,  Y10S516/929

摘要： The present invention relates to a method for manufacturing uniform size polymeric nanoparticles containing poorly soluble drugs, and more particularly, to a method for manufacturing uniform size polymeric nanoparticles containing poorly soluble drugs, including a first step of dissolving a biodegradable polymer in a non-volatile polar organic solvent, a second step of adding poorly soluble drugs to water and the biodegradable polymer solution to produce a dispersion, and a third step of adding the dispersion to emulsifier solutions in a batch under the condition of low mechanical energy level stirring. The polymeric nanoparticles of the present invention is capable of manufacturing nano-sized small and uniform polymeric nanoparticles through a simple method of employing a non-volatile polar solvent, especially a solvent having a polarity similar to that of water, as a solvent for a mixture solution of biodegradable polymer and poorly soluble materials, and using a low mechanical energy condition and batch-adding of dispersion in an emulsifying process. The polymeric nanoparticles of the present invention are advantageous in that the dissolution rate of the poorly soluble drugs contained in polymeric particles is dramatically improved, and the poorly soluble drugs are gradually and steadily released and maintained at a constant density over a long period of time.

摘要翻译： 本发明涉及一种制备均匀尺寸的含有难溶性药物的聚合物纳米颗粒的方法，更具体地说，涉及一种制备均匀尺寸的含有难溶性药物的聚合物纳米颗粒的方法，包括将可生物降解聚合物溶解在非挥发性物质中的第一步骤 极性有机溶剂，向水中添加难溶性药物和生物降解性聚合物溶液以产生分散体的第二步骤，以及在低机械能级搅拌的条件下将分散体分批加入到乳化剂溶液中的第三步骤。 本发明的聚合物纳米颗粒能够通过使用非挥发性极性溶剂，特别是极性类似于水的溶剂的简单方法作为混合物的溶剂来制造纳米尺寸的小而均匀的聚合物纳米颗粒 可生物降解聚合物和难溶性物质的溶液，并且使用低机械能条件和在乳化过程中分批添加分散体。 本发明的聚合物纳米颗粒的优点在于，包含在聚合物颗粒中的难溶性药物的溶解速度显着提高，并且难溶性药物在长时间内逐渐且稳定地释放并保持在恒定的密度。

公开(公告)号：US20110020225A1

公开(公告)日：2011-01-27

申请号：US12672517

申请日：2008-08-05

申请人： Bong Hyun Chung ,  Yong Taik Lim ,  Jung Hyun Han

发明人： Bong Hyun Chung ,  Yong Taik Lim ,  Jung Hyun Han

IPC分类号： A61K49/00 ,  A61K9/14 ,  A61P31/00

CPC分类号： A61K9/5031 ,  A61K49/0034 ,  A61K49/0054 ,  A61K49/0056 ,  A61K49/0089 ,  C08J3/12 ,  C08J2367/04

摘要： The present invention relates to porous polymer particles containing a charged molecule immobilized therein and a method for preparing the same. According to the disclosed invention, porous particles can be prepared using a biocompatible polymer and, at the same time, a charged molecule can be immobilized in the pores of the porous particles, such that various charged molecules can be loaded in the porous particles. In addition, various kinds of drugs or functional materials can be loaded into the porous particles of the present invention by electrostatic attraction and absorption or adsorption by a capillary phenomenon occurring due to porous properties.

摘要翻译： 本发明涉及含有固定在其中的带电分子的多孔聚合物颗粒及其制备方法。 根据所公开的发明，可以使用生物相容性聚合物制备多孔颗粒，并且同时可以将带电分子固定在多孔颗粒的孔中，使得各种带电分子可以负载在多孔颗粒中。 此外，通过由于多孔性质而发生的毛细管现象的静电吸引和吸收，可以将各种药物或功能材料装载到本发明的多孔颗粒中。

公开(公告)号：US08734762B2

公开(公告)日：2014-05-27

申请号：US12738394

申请日：2008-08-20

申请人： Bong Hyun Chung ,  Yong Taik Lim ,  Bang Sil Choi ,  Jung Hyun Han

发明人： Bong Hyun Chung ,  Yong Taik Lim ,  Bang Sil Choi ,  Jung Hyun Han

IPC分类号： A61B5/00 ,  A61B8/00 ,  A61K9/66

CPC分类号： C09K11/565 ,  C09K11/02 ,  C09K11/883

摘要： The present invention relates to a perfluorocarbon nanoemulsion containing quantum dot nanoparticles and a method for preparing the same, more specifically, relates to a method for preparing a multifunctional perfluorocarbon nanoemulsion which simultaneously shows the fluorine-based magnetic resonance properties of perfluorocarbon and the optical properties of quantum dot nanoparticles, the method comprising dispersing quantum dot nanoparticles, modified with a fluorine end group, in perfluorocarbon, and then adding an aqueous emulsifier solution containing lipid to the dispersion. The disclosed perfluorocarbon nanoemulsion containing quantum dot nanoparticles has excellent biocompatibility and simultaneously shows optical properties and magnetic resonance properties, and thus can be used as a contrast agent for multi-mode molecular imaging. Accordingly, the nanoemulsion can be used to study cell and small-animal molecular imaging in various fields, including biological and medical fields.

摘要翻译： 本发明涉及含有量子点纳米粒子的全氟碳纳米乳液及其制备方法，更具体地涉及制备多功能全氟碳纳米乳液的方法，该方法同时显示全氟化碳的氟基磁共振性能和光学性能 量子点纳米粒子，该方法包括将用氟端基改性的量子点纳米粒子分散在全氟化碳中，然后向分散体中加入含有脂质的水性乳化剂溶液。 所公开的含有量子点纳米粒子的全氟化碳纳米乳液具有优异的生物相容性，并同时显示光学性质和磁共振性质，因此可用作多模式分子成像的造影剂。 因此，纳米乳液可用于研究包括生物和医学领域在内的各个领域的细胞和小动物分子成像。

公开(公告)号：US20100233094A1

公开(公告)日：2010-09-16

申请号：US12738394

申请日：2008-08-20

申请人： Bong Hyun Chung ,  Yong Taik Lim ,  Bang Sil Choi ,  Jung Hyun Han

发明人： Bong Hyun Chung ,  Yong Taik Lim ,  Bang Sil Choi ,  Jung Hyun Han

IPC分类号： A61B5/055

CPC分类号： C09K11/565 ,  C09K11/02 ,  C09K11/883

摘要： The present invention relates to a perfluorocarbon nanoemulsion containing quantum dot nanoparticles and a method for preparing the same, more specifically, relates to a method for preparing a multifunctional perfluorocarbon nanoemulsion which simultaneously shows the fluorine-based magnetic resonance properties of perfluorocarbon and the optical properties of quantum dot nanoparticles, the method comprising dispersing quantum dot nanoparticles, modified with a fluorine end group, in perfluorocarbon, and then adding an aqueous emulsifier solution containing lipid to the dispersion. The disclosed perfluorocarbon nanoemulsion containing quantum dot nanoparticles has excellent biocompatibility and simultaneously shows optical properties and magnetic resonance properties, and thus can be used as a contrast agent for multi-mode molecular imaging. Accordingly, the nanoemulsion can be used to study cell and small-animal molecular imaging in various fields, including biological and medical fields.

摘要翻译： 本发明涉及含有量子点纳米粒子的全氟碳纳米乳液及其制备方法，更具体地涉及制备多功能全氟碳纳米乳液的方法，该方法同时显示全氟化碳的氟基磁共振性能和光学性能 量子点纳米粒子，该方法包括将用氟端基改性的量子点纳米粒子分散在全氟化碳中，然后向分散体中加入含有脂质的水性乳化剂溶液。 所公开的含有量子点纳米粒子的全氟化碳纳米乳液具有优异的生物相容性，并同时显示光学性质和磁共振性质，因此可用作多模式分子成像的造影剂。 因此，纳米乳液可用于研究包括生物和医学领域在内的各个领域的细胞和小动物分子成像。

公开(公告)号：US08697389B2

公开(公告)日：2014-04-15

申请号：US12937875

申请日：2009-03-24

申请人： Bong Hyun Chung ,  Yong Taik Lim

发明人： Bong Hyun Chung ,  Yong Taik Lim

IPC分类号： C12Q1/02

CPC分类号： G01N33/532 ,  B82Y15/00 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/587 ,  G01N33/588

摘要： The present invention relates to cell labeling and imaging using a multifunctional perfluorocarbon (PFC) nanoemulsion. In particular, the perfluorocarbon nanoemulsion containing optical nanoparticle is provided with both optical characteristics and 19F magnetic resonance characteristics, thus can be used for magnetic resonance imaging (MRI) and optical imaging (OI) simultaneously. Cell labeling and imaging using the perfluorocarbon nanoemulsion containing quantum dot nanoparticle exerted no effect on cell viability. Therefore, perfluorocarbon nanoemulsion can be applied to methods for multifunctional and effectively cell labeling and imaging.

摘要翻译： 本发明涉及使用多功能全氟化碳（PFC）纳米乳液的细胞标记和成像。 特别是，含有光学纳米颗粒的全氟化碳纳米乳液具有光学特性和19F磁共振特性，因此可同时用于磁共振成像（MRI）和光学成像（OI）。 使用含有量子点纳米颗粒的全氟化碳纳米乳液的细胞标记和成像对细胞活力没有影响。 因此，全氟化碳纳米乳液可用于多功能，有效的细胞标记和成像方法。

公开(公告)号：US20120088261A1

公开(公告)日：2012-04-12

申请号：US12937875

申请日：2009-03-24

申请人： Bong Hyun Chung ,  Yong Taik Lim

发明人： Bong Hyun Chung ,  Yong Taik Lim

IPC分类号： C12Q1/02 ,  B82Y5/00

CPC分类号： G01N33/532 ,  B82Y15/00 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/587 ,  G01N33/588

摘要： The present invention relates to cell labeling and imaging using a multifunctional perfluorocarbon (PFC) nanoemulsion. In particular, the perfluorocarbon nanoemulsion containing optical nanoparticle is provided with both optical characteristics and 19F magnetic resonance characteristics, thus can be used for magnetic resonance imaging (MRI) and optical imaging (OI) simultaneously. Cell labeling and imaging using the perfluorocarbon nanoemulsion containing quantum dot nanoparticle exerted no effect on cell viability. Therefore, perfluorocarbon nanoemulsion can be applied to methods for multifunctional and effectively cell labeling and imaging.

摘要翻译： 本发明涉及使用多功能全氟化碳（PFC）纳米乳液的细胞标记和成像。 特别是，含有光学纳米颗粒的全氟化碳纳米乳液具有光学特性和19F磁共振特性，因此可同时用于磁共振成像（MRI）和光学成像（OI）。 使用含有量子点纳米颗粒的全氟化碳纳米乳液的细胞标记和成像对细胞活力没有影响。 因此，全氟化碳纳米乳液可用于多功能，有效的细胞标记和成像方法。

公开(公告)号：US20110110867A1

公开(公告)日：2011-05-12

申请号：US13000197

申请日：2009-06-19

申请人： Bong Hyun Chung ,  Yong Taik Lim

发明人： Bong Hyun Chung ,  Yong Taik Lim

IPC分类号： A61K49/06 ,  A61K49/00 ,  B82Y15/00

CPC分类号： A61K49/0078 ,  A61K49/0002 ,  A61K49/0017 ,  A61K49/0067 ,  A61K49/1806

摘要： The present invention relates to a multimodal imaging method using a nano-emulsion comprising optical nano-particles and perfluorocarbons, and more particularly, to a method of obtaining multimodal images using a nano-emulsion consisted of perfluorocarbons for multispectral magnetic resonance imaging (MRI) and nano-particles for multicolor fluorescent detection. In the multimodal imaging method, various multispectral MRI images and multicolor fluorescent rays can be obtained by varying the kind and combination of perfluorocarbons and the kind and combination of optical nano-particles, so that multiplexed analysis is possible. Accordingly, the multimodal imaging method can be applied to various fields, including biological and medical fields in which studies on cell molecular imaging has been conducted.

摘要翻译： 本发明涉及使用包含光学纳米颗粒和全氟化碳的纳米乳液的多模式成像方法，更具体地说，涉及使用由全氟化碳组成的纳米乳液用于多光谱磁共振成像（MRI）和 用于多色荧光检测的纳米颗粒。 在多模式成像方法中，通过改变全氟化碳的种类和组合以及光学纳米颗粒的种类和组合，可以获得各种多光谱MRI图像和多色荧光光谱，从而可以进行多重分析。 因此，多模式成像方法可以应用于已经进行细胞分子成像研究的生物和医学领域的各个领域。

公开(公告)号：US20100204444A1

公开(公告)日：2010-08-12

申请号：US12670245

申请日：2008-07-25

申请人： Bong Hyun Chung ,  Yong Taik Lim ,  Mi Young Cho ,  Jeong Min Lee

发明人： Bong Hyun Chung ,  Yong Taik Lim ,  Mi Young Cho ,  Jeong Min Lee

IPC分类号： C07K16/00 ,  C07K2/00 ,  C12N9/96 ,  C07K7/06 ,  C07H21/00 ,  C12N15/63 ,  C12P21/06 ,  C12N1/00

CPC分类号： C07K14/31 ,  C07K2319/03 ,  C07K2319/09

摘要： The present invention relates to a polypeptide structure which can simultaneously deliver an antibody and a nanoparticle into cells, more specifically, to a polypeptide structure for intracellular delivery of an antibody and a nanoparticle, which comprises a nanoparticle-binding region, an antibody-binding region and a signaling capable of delivering substances into cells.

摘要翻译： 本发明涉及一种多肽结构，其可以将抗体和纳米颗粒同时递送到细胞中，更具体地涉及用于细胞内递送抗体和纳米颗粒的多肽结构，其包含纳米颗粒结合区，抗体结合区 以及能够将物质输送到细胞中的信号。

公开(公告)号：US08753606B2

公开(公告)日：2014-06-17

申请号：US13000197

申请日：2009-06-19

申请人： Bong Hyun Chung ,  Yong Taik Lim

发明人： Bong Hyun Chung ,  Yong Taik Lim

IPC分类号： A61K49/00

CPC分类号： A61K49/0078 ,  A61K49/0002 ,  A61K49/0017 ,  A61K49/0067 ,  A61K49/1806

摘要： The present invention relates to a multimodal imaging method using a nano-emulsion comprising optical nano-particles and perfluorocarbons, and more particularly, to a method of obtaining multimodal images using a nano-emulsion consisted of perfluorocarbons for multispectral magnetic resonance imaging (MRI) and nano-particles for multicolor fluorescent detection. In the multimodal imaging method, various multispectral MRI images and multicolor fluorescent rays can be obtained by varying the kind and combination of perfluorocarbons and the kind and combination of optical nano-particles, so that multiplexed analysis is possible. Accordingly, the multimodal imaging method can be applied to various fields, including biological and medical fields in which studies on cell molecular imaging has been conducted.

摘要翻译： 本发明涉及使用包含光学纳米颗粒和全氟化碳的纳米乳液的多模式成像方法，更具体地说，涉及使用由全氟化碳组成的纳米乳液用于多光谱磁共振成像（MRI）和 用于多色荧光检测的纳米颗粒。 在多模式成像方法中，通过改变全氟化碳的种类和组合以及光学纳米颗粒的种类和组合，可以获得各种多光谱MRI图像和多色荧光光谱，从而可以进行多重分析。 因此，多模式成像方法可以应用于已经进行细胞分子成像研究的生物和医学领域的各个领域。