公开(公告)号：US20200283806A1

公开(公告)日：2020-09-10

申请号：US16796417

申请日：2020-02-20

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Lucas Pedersen PARIZZI ,  Felipe GALZERANI

IPC: C12P7/18 ,  C12P17/02 ,  C12N15/52 ,  C12N1/20 ,  C12P7/28 ,  C12P5/02

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG), or optionally MEG and one or more co-product, from one or more hexose feedstock. The present application also relates to recombinant microorganisms useful in the biosynthesis of glycolic acid (GA), or optionally GA and one or more co-product, from one or more hexose feedstock. The present application relates to recombinant microorganisms useful in the biosynthesis of xylitol, or optionally xylitol and one or more co-product, from one or more hexose feedstock. Also provided are methods of producing MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product, from one or more hexose feedstock using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product.

公开(公告)号：US20250075235A1

公开(公告)日：2025-03-06

申请号：US18952535

申请日：2024-11-19

Applicant: Braskem S.A.

Inventor： Felipe GALZERANI ,  Adler Gomes MOURA ,  Mateus Schreiner GARCEZ LOPES ,  Luige Armando Lierena CALDERON

IPC: C12P7/06 ,  B01D3/14 ,  C12N1/18 ,  C12P7/16 ,  C12P7/28 ,  C12R1/865

Abstract: The disclosure provides processes for the production of ethanol and one or more co-products from a fermentable carbon source. The ethanol and one or more co-products are produced in an ethanol-producing yeast modified to further produce the one or more co-products. The processes involve contacting a fermentable carbon source with the modified yeast in a fermentation medium, fermenting the yeast in the fermentation medium such that the yeast produces ethanol and the one or more co-products from the fermentable carbon source, and isolating the ethanol and the one or more co-products. The modified yeast is an ethanol-producing yeast that produces ethanol in a greater concentration than the one or more co-products. Additionally, the disclosure provides the modified yeast disclosed herein.

公开(公告)号：US20200216864A1

公开(公告)日：2020-07-09

申请号：US16719833

申请日：2019-12-18

Applicant: Braskem S.A.

Inventor： Beatriz Leite MAGALHAES ,  Paulo Moises Raduan ALEXANDRINO ,  Felipe GALZERANI

IPC: C12P7/42 ,  C12N1/16

Abstract: The present disclosure provides methods for utilizing genetically modified microbes to co-produce 3-hydroxypropionic acid (3-HP) and acetyl-CoA, and derivatives thereof from malonate semialdehyde as a common single intermediate. The disclosure further provides modified microbe that co-produce the 3-HP and acetyl-CoA derivatives from malonate semialdehyde.

公开(公告)号：US20230037707A1

公开(公告)日：2023-02-09

申请号：US17856863

申请日：2022-07-01

Applicant: Braskem S.A.

Inventor： Beatriz Leite MAGALHAES ,  Paulo Moises Raduan ALEXANDRINO ,  Felipe GALZERANI

IPC: C12P7/42 ,  C12N1/16

Abstract: The present disclosure provides methods for utilizing genetically modified microbes to co-produce 3-hydroxypropionic acid (3-HP) and acetyl-CoA, and derivatives thereof from malonate semialdehyde as a common single intermediate. The disclosure further provides modified microbe that co-produce the 3-HP and acetyl-CoA derivatives from malonate semialdehyde.

公开(公告)号：US20200283811A1

公开(公告)日：2020-09-10

申请号：US16796426

申请日：2020-02-20

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Lucas Pederson PARIZZI ,  Felipe GALZERANI ,  Jean Marie FRANCOIS ,  Sophie LAJUS

IPC: C12P17/18 ,  C12P17/02 ,  C12N15/52 ,  C12N1/20 ,  C12P7/28 ,  C12P5/02

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) or glycolic acid (GA), or MEG and one or more co-product, from one or more pentose and/or hexose sugars. Also provided are methods of producing MEG (or GA), or MEG (or GA) and one or more co-product, from one or more pentose and/or hexose sugars using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA), or MEG and one or more co-product.

公开(公告)号：US20240392329A1

公开(公告)日：2024-11-28

申请号：US18737619

申请日：2024-06-07

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Lucas Pedersen PARIZZI ,  Felipe GALZERANI

IPC: C12P7/18 ,  C12N1/20 ,  C12N15/52 ,  C12P5/02 ,  C12P7/28 ,  C12P17/02 ,  C12R1/19

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG), or optionally MEG and one or more co-product, from one or more hexose feedstock. The present application also relates to recombinant microorganisms useful in the biosynthesis of glycolic acid (GA), or optionally GA and one or more co-product, from one or more hexose feedstock. The present application relates to recombinant microorganisms useful in the biosynthesis of xylitol, or optionally xylitol and one or more co-product, from one or more hexose feedstock. Also provided are methods of producing MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product, from one or more hexose feedstock using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product.

公开(公告)号：US20240067994A1

公开(公告)日：2024-02-29

申请号：US18237170

申请日：2023-08-23

Applicant: Braskem S.A.

Inventor： Renata PINTO DA SILVA MATOS ,  Thiago BEZERRA TAKETA ,  Felipe GALZERANI ,  Adler GOMES MOURA

IPC: C12P7/06 ,  B01D3/34 ,  B01D3/40 ,  C12N1/16 ,  C12P7/28

CPC classification number: C12P7/06 ,  B01D3/343 ,  B01D3/40 ,  C12N1/16 ,  C12P7/28

Abstract: The disclosure provides a process for the production of ethanol and one or more low boiling compound from a fermentable carbon source. The ethanol and the low boiling compound(s) are produced using an ethanol-producing yeast modified to further produce the one or more low boiling point compounds. In one embodiment, the low boiling compound(s) are acetone, 1-propanol, and/or 2-propanol. Additionally, the disclosure provides a process for the isolation ad purification of the one or more low boiling compounds from ethanol.

公开(公告)号：US20220380815A1

公开(公告)日：2022-12-01

申请号：US17830229

申请日：2022-06-01

Applicant: Braskem S.A.

Inventor： Felipe GALZERANI ,  Bianca Bassetto BISSONI ,  Karine JAILLARDON ,  Dominique LOUIS

IPC: C12P7/40 ,  C12N15/52 ,  C12N1/18 ,  C12N15/81

Abstract: The present disclosure provides recombinant microorganisms and methods for producing malonate semialdehyde and/or related products, such as ketones, alcohols, organic acids, esters, alkenes, amino acids, and combinations thereof including 3-hydroxypropionic acid, acrylic acid, propionic acid, 1-propanol, acetone, 2-propanol, butanone, 1-butanol, 2-butanol, methyl propionate, 1,3-propanediol, isoamyl alcohol, 1,3-butanediol, 1,4-butanediol, 2,3-butanediol, lactic acid, adipic acid, glutamic acid, itaconic acid, ethyl acetate, isopropyl acetate, acetic acid, butyric acid, caproic acid, citric acid, methacrylic acid, succinic acid, propylene, butadiene, ethanol, isoprenol, leucine, isoleucine, glutamine, glycine, and isoprene, from β-alanine. The recombinant microorganism expresses an asparate 1-decarboxylase that catalyzes the production of malonate semialdehyde from β-alanine.

公开(公告)号：US20210261987A1

公开(公告)日：2021-08-26

申请号：US17182061

申请日：2021-02-22

Applicant: Braskem S.A.

Inventor： Felipe GALZERANI ,  Adler Gomes MOURA ,  Mateus Schreiner GARCEZ LOPES ,  Luige Armando Lierena CALDERON

IPC: C12P7/06 ,  C12P7/16 ,  C12P7/28

Abstract: The disclosure provides processes for the production of ethanol and one or more co-products from a fermentable carbon source. The ethanol and one or more co-products are produced in an ethanol-producing yeast modified to further produce the one or more co-products. The processes involve contacting a fermentable carbon source with the modified yeast in a fermentation medium, fermenting the yeast in the fermentation medium such that the yeast produces ethanol and the one or more co-products from the fermentable carbon source, and isolating the ethanol and the one or more co-products. The modified yeast is an ethanol-producing yeast that produces ethanol in a greater concentration than the one or more co-products. Additionally, the disclosure provides the modified yeast disclosed herein.

公开(公告)号：US20200263210A1

公开(公告)日：2020-08-20

申请号：US16791556

申请日：2020-02-14

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Felipe GALZERANI ,  Paulo Moises Raduan ALEXANDRINO

IPC: C12P7/42 ,  C12P13/04 ,  C12N9/00 ,  C12N9/88

Abstract: The present invention provides biochemical pathways, glyoxylate producing recombinant microorganisms, and methods for the production and yield improvement of glycolic acid and/or glycine via a reverse glyoxylate shunt. The reverse glyoxylate shunt comprises an enzyme that catalyzes the carboxylation of phosphoenol pyruvate (PEP) to oxaloacetate (OAA), or an enzyme that catalyzes the carboxylation of pyruvate to oxaloacetate (OAA) or an enzyme that catalyzes the carboxylation of pyruvate to malate or a combination of any of the previous reactions; an enzyme that catalyzes the conversion of malate to malyl-CoA; an enzyme that catalyzes the conversion of malyl-CoA to glyoxylate and acetyl-CoA; and optionally an enzyme that catalyzes the conversion of oxaloacetate (OAA) to malate. Glyoxylate is reduced to produce glycolate. Alternatively, glyoxylate is converted to glycine. The reverse glyoxylate shunt pathway of the present invention can be utilized synergistically with other glycolic acid and/or glycine producing pathways to increase product yield.