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    Method for mutation detection in HIV-1 using pol sequencing
    1.
    发明申请
    Method for mutation detection in HIV-1 using pol sequencing 审中-公开
    使用pol测序的HIV-1突变检测方法

    公开(公告)号：US20070287834A1

    公开(公告)日：2007-12-13

    申请号：US11803541

    申请日：2007-05-14

    申请人： Brendan Larder ,  Sharon Kemp ,  Stuart Bloor ,  Ann Brophy

    发明人： Brendan Larder ,  Sharon Kemp ,  Stuart Bloor ,  Ann Brophy

    IPC分类号： C07H21/04

    CPC分类号： C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q1/703 ,  C12Q2537/125 ,  C12Q2523/101 ,  C12Q2549/113

    摘要： The present invention relates to a method for mutation analysis of the HIV pol gene of HIV-1 virions comprising amplifying virion RNA or DNA via nested PCR using outer primers as represented in SEQ ID No. 1 and 2, amplifying said PCR product via nested PCR using a 5′ and 3′ primer chosen from the inner primers SEQ ID No. 3, 4, 5 and 6, and sequencing this secondary obtained PCR product using at least one sequencing primer chosen from any of SEQ ID No. 7 to 12 or variants thereof. In the alternative, at least one secondary sequencing primer may be used chosen from any of SEQ ID No. 13 to 24. The present invention also relates to kits for performing such a method as well as primers for performing the same.

    摘要翻译： 本发明涉及HIV-1病毒体的HIV pol基因的突变分析方法，其包括使用SEQ ID No.1和2所示的外部引物通过巢式PCR扩增病毒粒子RNA或DNA，通过巢式PCR扩增所述PCR产物 使用选自内部引物SEQ ID No.3,4,5和6的5'和3'引物，并使用至少一种选自SEQ ID No.7至12中的任一个的测序引物或该序列获得的PCR产物 其变体。 在替代方案中，可以使用从SEQ ID No.13至24中任一个选择的至少一个二次测序引物。本发明还涉及用于进行该方法的试剂盒以及用于进行该方法的引物。

    Method for mutation detection in HIV-1 using pol sequencing
    2.
    发明授权
    Method for mutation detection in HIV-1 using pol sequencing 有权
    使用pol测序的HIV-1突变检测方法

    公开(公告)号：US06800463B1

    公开(公告)日：2004-10-05

    申请号：US09640787

    申请日：2000-08-18

    申请人： Brendan Larder ,  Sharon Kemp ,  Stuart Bloor ,  Ann Brophy

    发明人： Brendan Larder ,  Sharon Kemp ,  Stuart Bloor ,  Ann Brophy

    IPC分类号： C12P1934

    CPC分类号： C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q1/703 ,  C12Q2537/125 ,  C12Q2523/101 ,  C12Q2549/113

    摘要： The present invention relates to a method of for mutation analysis of the HIV pol gene of HIV-1 virions comprising amplifying viral RNA or DNA via nested PCR using outer primers as represented in SEQ ID No: 1 and 2, amplifying said PCR product via nested PCR using a 5′ and 3′ primer chosen from the inner primers SEQ ID No: 3, 4, 5, and 6, and sequencing this secondary obtained PCR product using at least one sequencing primer chosen from any of SEQ ID No: 7 to 12 or variants thereof. In the alternative, at least one secondary sequencing primer may be used chosen from any of SEQ ID No: 13 to 24. The present invention also relates to kits for performing such a method as well as primers for performing the same.

    摘要翻译： 本发明涉及HIV-1病毒粒子的HIV pol基因的突变分析方法，其包括使用SEQ ID No：1和2所示的外部引物通过巢式PCR扩增病毒RNA或DNA，通过嵌套扩增所述PCR产物 使用选自内部引物SEQ ID No：3,4,5和6的5'和3'引物进行PCR，并使用至少一种选自SEQ ID No：7至 12或其变体。 在替代方案中，可以使用从SEQ ID No：13至24中任一个选择的至少一个二次测序引物。本发明还涉及用于进行该方法的试剂盒以及用于进行该方法的引物。

    Method for mutation detection in HIV-1 using pol sequencing
    3.
    发明授权
    Method for mutation detection in HIV-1 using pol sequencing 有权
    使用pol测序的HIV-1突变检测方法

    公开(公告)号：US07235387B2

    公开(公告)日：2007-06-26

    申请号：US10258151

    申请日：2001-04-20

    申请人： Brendan Larder ,  Sharon Kemp ,  Stuart Bloor ,  Ann Brophy

    发明人： Brendan Larder ,  Sharon Kemp ,  Stuart Bloor ,  Ann Brophy

    IPC分类号： C12Q1/68 ,  C12Q1/70

    CPC分类号： C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q1/703 ,  C12Q2537/125 ,  C12Q2523/101 ,  C12Q2549/113

    摘要： The present invention relates to a method for mutation analysis of the HIV pol gene of HIV virions comprising amplifying virion RNA or DNA via nested PCR using outer primers as represented in SEQ ID No. 1 and 2, amplifying said PCR product via nested PCR using a 5′ and 3′ primer chosen from the inner primers SEQ ID No. 3, 4, 5, and 6, and sequencing this secondary obtained PCR product using at least one sequencing primer chosen from any of SEQ ID No. 7 to 12 or variants thereof. In the alternative, at least one secondary sequencing primer may be used chosen from any of SEQ ID No. 13 to 24. The benefit of the sequences present in the invention resides in the fact that, with the aid of the oligonucleotides, the sequences of all presently known HIV subtypes and all mutations of the pol gene presently known to yield resistance towards antiretroviral therapy can be determined. The present invention also relates to kits for performing such a method as well as primers for performing the same.

    摘要翻译： 本发明涉及HIV病毒体的HIV pol基因的突变分析方法，其包括使用SEQ ID No.1和2所示的外部引物通过巢式PCR扩增病毒体RNA或DNA，通过巢式PCR扩增所述PCR产物，使用 选自内引物SEQ ID No.3,4,5和6的5'和3'引物，并使用至少一种选自SEQ ID No.7至12或任何变体的测序引物对该次级获得的PCR产物进行测序 其中。 在替代方案中，可以使用从SEQ ID No.13至24中任一个中选择的至少一个二次测序引物。存在于本发明中的序列的益处在于：借助于寡核苷酸， 目前已知所有目前已知的HIV亚型和目前已知对抗逆转录病毒治疗产生抗性的pol基因的所有突变可以被确定。 本发明还涉及用于进行这种方法的试剂盒以及用于进行该方法的引物。

    Method for mutation detection in HIV-1 using pol sequencing
    4.
    发明申请
    Method for mutation detection in HIV-1 using pol sequencing 审中-公开
    使用pol测序的HIV-1突变检测方法

    公开(公告)号：US20050064397A1

    公开(公告)日：2005-03-24

    申请号：US10935975

    申请日：2004-09-08

    申请人： Brendan Larder ,  Sharon Kemp ,  Stuart Bloor ,  Ann Brophy

    发明人： Brendan Larder ,  Sharon Kemp ,  Stuart Bloor ,  Ann Brophy

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12Q1/70 ,  C12P19/34

    CPC分类号： C12Q1/703

    摘要： The present invention relates to a method of for mutation analysis of the HIV pol gene of HIV-1 virions comprising amplifying viral RNA or DNA via nested PCR using outer primers as represented in SEQ ID No: 1 and 2, amplifying said PCR product via nested PCR using a 5′ and 3′ primer chosen from the inner primers SEQ ID No: 3, 4, 5, and 6, and sequencing this secondary obtained PCR product using at least one sequencing primer chosen from any of SEQ ID No: 7 to 12 or variants thereof. In the alternative, at least one secondary sequencing primer may be used chosen from any of SEQ ID No: 13 to 24. The present invention also relates to kits for performing such a method as well as primers for performing the same.

    摘要翻译： 本发明涉及HIV-1病毒粒子的HIV pol基因的突变分析方法，其包括使用SEQ ID No：1和2所示的外部引物通过巢式PCR扩增病毒RNA或DNA，通过嵌套扩增所述PCR产物 使用选自内部引物SEQ ID No：3,4,5和6的5'和3'引物进行PCR，并使用至少一种选自SEQ ID No：7至 12或其变体。 在替代方案中，可以使用从SEQ ID No：13至24中任一个选择的至少一个二次测序引物。本发明还涉及用于进行该方法的试剂盒以及用于进行该方法的引物。

    Method for mutation detection in hiv using pol sequencing
    5.
    发明申请
    Method for mutation detection in hiv using pol sequencing 有权
    使用pol测序的hiv突变检测方法

    公开(公告)号：US20050058981A1

    公开(公告)日：2005-03-17

    申请号：US10258151

    申请日：2001-04-20

    申请人： Brendan Larder ,  Sharon Kemp

    发明人： Brendan Larder ,  Sharon Kemp

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12Q1/70

    CPC分类号： C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q1/703 ,  C12Q2537/125 ,  C12Q2523/101 ,  C12Q2549/113

    摘要： The present invention relates to a method for mutation analysis of the HIV pol gene of HIV virions comprising amplifying virion RNA or DNA via nested PCR using outer primers as represented in SEQ ID No. 1 and 2, amplifying said PCR product via nested PCR using a 5′ and 3′ primer chosen from the inner primers SEQ ID No. 3, 4, 5, and 6, and sequencing this secondary obtained PCR product using at least one sequencing primer chosen from any of SEQ ID No. 7 to 12 or variants thereof. In the alternative, at least one secondary sequencing primer may be used chosen from any of SEQ ID No. 13 to 24. The benefit of the sequences present in the invention resides in the fact that, with the aid of the oligonucleotides, the sequences of all presently known HIV subtypes and all mutations of the pol gene presently known to yield resistance towards antiretroviral therapy can be determined. The present invention also relates to kits for performing such a method as well as primers for performing the same.

    摘要翻译： 本发明涉及HIV病毒体的HIV pol基因的突变分析方法，其包括使用SEQ ID No.1和2所示的外部引物通过巢式PCR扩增病毒体RNA或DNA，通过巢式PCR扩增所述PCR产物，使用 选自内引物SEQ ID No.3,4,5和6的5'和3'引物，并使用至少一种选自SEQ ID No.7至12或任何变体的测序引物对该次级获得的PCR产物进行测序 其中。 在替代方案中，可以使用从SEQ ID No.13至24中任一个中选择的至少一个二次测序引物。存在于本发明中的序列的益处在于：借助于寡核苷酸， 目前已知所有目前已知的HIV亚型和目前已知对抗逆转录病毒治疗产生抗性的pol基因的所有突变可以被确定。 本发明还涉及用于进行这种方法的试剂盒以及用于进行该方法的引物。

    End-board for a core-wound roll product packaging system
    9.
    发明申请
    End-board for a core-wound roll product packaging system 失效
    用于卷芯卷产品包装系统的端板

    公开(公告)号：US20050133659A1

    公开(公告)日：2005-06-23

    申请号：US10745351

    申请日：2003-12-22

    申请人： Sharon Kemp ,  Cheryl Wetmore

    发明人： Sharon Kemp ,  Cheryl Wetmore

    IPC分类号： B65D85/66 ,  B65D85/672 ,  B65H49/32

    CPC分类号： B65D85/672

    摘要： An end-board is provided for supporting and handling a core-wound roll of material, such as a roll of polymer film. The end-board generally comprises a support plate comprising a product face, an outer face, a core support aperture, a core channel, and the core support aperture for supporting a core plug or a core therein. The product face comprises a roll recess for receiving an end of the core-wound roll within the roll recess when the core plug or core is positioned in the core support aperture. A locking collar may also be provided to selectively engage the support plate in the support plate core channel to prevent the supported core or core plug from passing through the core channel. A pair of handles may also be provided within the support plate to facilitate end-board manipulation.

    摘要翻译： 提供端板，用于支撑和处理诸如聚合物膜卷的材料卷绕卷材。 端板通常包括支撑板，该支撑板包括产品表面，外表面，芯支撑孔，芯通道和用于在其中支撑芯塞或芯体的芯支撑孔。 产品表面包括一个辊凹槽，用于当芯塞或芯位于芯支撑孔中时，将卷绕的辊的端部接收在辊凹槽内。 还可以提供锁定环以选择性地接合支撑板芯通道中的支撑板，以防止受支撑的芯或芯塞通过芯通道。 还可以在支撑板内设置一对把手以便于终板操作。