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公开(公告)号：US07206699B2
公开(公告)日：2007-04-17
申请号：US09836477
申请日：2001-04-18
申请人： Brendan Larder , Stuart Bloor , Kurt Hertogs , Pascale Alfons Rosa Dehertogh , Rudy Jean Marc Mortier
发明人： Brendan Larder , Stuart Bloor , Kurt Hertogs , Pascale Alfons Rosa Dehertogh , Rudy Jean Marc Mortier
IPC分类号： G06F19/00
CPC分类号： C12Q1/6876 , C12Q1/703 , C12Q1/707 , C12Q2600/156 , G06F19/18 , G06F19/22 , G06F19/24 , G06F19/28
摘要： The present invention concerns methods for measuring drug resistance by correlating genotypic information with phenotypic profiles. In one embodiment, a method for interpreting genotypic information is described wherein a genetic code is generated from a patient sample, a list of mutations known or suspect to play a role in the development of resistance to one or more drugs is obtained from the generated genetic code, a genotype database is interrogated for previous samples with similar mutations relating to said one or more drugs, a phenotype for said sample is located in a phenotype database, the mean change in inhibition is determined based on all the examples located in said phenotype database, a distribution of sensitivities of one or more drugs suitable for treating a specific indication is determined.
摘要翻译： 本发明涉及通过将基因型信息与表型谱相关联来测量耐药性的方法。 在一个实施方案中，描述了一种用于解释基因型信息的方法，其中从患者样品产生遗传密码，从产生的遗传基因获得已知或怀疑在发展对一种或多种药物的抗性发展中起作用的突变的列表 对于先前具有与所述一种或多种药物相似的突变的样品，询问基因型数据库，所述样品的表型位于表型数据库中，基于位于所述表型数据库中的所有实施例确定抑制的平均变化 确定一种或多种适合于治疗特定指征的药物的灵敏度分布。
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公开(公告)号：US20070166747A1
公开(公告)日：2007-07-19
申请号：US11682749
申请日：2007-03-06
申请人： Brendan Larder , Stuart Bloor , Kurt Hertogs , Pascale Dehertogh , Rudy Mortier
发明人： Brendan Larder , Stuart Bloor , Kurt Hertogs , Pascale Dehertogh , Rudy Mortier
CPC分类号： C12Q1/6876 , C12Q1/703 , C12Q1/707 , C12Q2600/156 , G16B20/00 , G16B30/00 , G16B40/00 , G16B50/00
摘要： The present invention concerns methods for measuring drug resistance by correlating genotypic information with phenotypic profiles. In one embodiment, a method for interpreting genotypic information is described wherein a genetic code is generated from a patient sample, a list of mutations known or suspect to play a role in the development of resistance to one or more drugs is obtained from the generated genetic code, a genotype database is interrogated for previous samples with similar mutations relating to said one or more drugs, a phenotype for said sample is located in a phenotype database, the mean change in inhibition is determined based on all the examples located in said phenotype database, a distribution of sensitivities of one or more drugs suitable for treating a specific indication is determined.
摘要翻译： 本发明涉及通过将基因型信息与表型谱相关联来测量耐药性的方法。 在一个实施方案中，描述了一种用于解释基因型信息的方法，其中从患者样品产生遗传密码，从产生的遗传基因获得已知或怀疑在发展对一种或多种药物的抗性发展中起作用的突变的列表 对于先前具有与所述一种或多种药物相似的突变的样品，询问基因型数据库，所述样品的表型位于表型数据库中，基于位于所述表型数据库中的所有实施例确定抑制的平均变化 确定一种或多种适合于治疗特定指征的药物的灵敏度分布。
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公开(公告)号：US20070287834A1
公开(公告)日：2007-12-13
申请号：US11803541
申请日：2007-05-14
申请人： Brendan Larder , Sharon Kemp , Stuart Bloor , Ann Brophy
发明人： Brendan Larder , Sharon Kemp , Stuart Bloor , Ann Brophy
IPC分类号： C07H21/04
CPC分类号： C12Q1/6837 , C12Q1/6858 , C12Q1/6869 , C12Q1/703 , C12Q2537/125 , C12Q2523/101 , C12Q2549/113
摘要： The present invention relates to a method for mutation analysis of the HIV pol gene of HIV-1 virions comprising amplifying virion RNA or DNA via nested PCR using outer primers as represented in SEQ ID No. 1 and 2, amplifying said PCR product via nested PCR using a 5′ and 3′ primer chosen from the inner primers SEQ ID No. 3, 4, 5 and 6, and sequencing this secondary obtained PCR product using at least one sequencing primer chosen from any of SEQ ID No. 7 to 12 or variants thereof. In the alternative, at least one secondary sequencing primer may be used chosen from any of SEQ ID No. 13 to 24. The present invention also relates to kits for performing such a method as well as primers for performing the same.
摘要翻译： 本发明涉及HIV-1病毒体的HIV pol基因的突变分析方法，其包括使用SEQ ID No.1和2所示的外部引物通过巢式PCR扩增病毒粒子RNA或DNA，通过巢式PCR扩增所述PCR产物 使用选自内部引物SEQ ID No.3,4,5和6的5'和3'引物，并使用至少一种选自SEQ ID No.7至12中的任一个的测序引物或该序列获得的PCR产物 其变体。 在替代方案中，可以使用从SEQ ID No.13至24中任一个选择的至少一个二次测序引物。本发明还涉及用于进行该方法的试剂盒以及用于进行该方法的引物。
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公开(公告)号：US06800463B1
公开(公告)日：2004-10-05
申请号：US09640787
申请日：2000-08-18
申请人： Brendan Larder , Sharon Kemp , Stuart Bloor , Ann Brophy
发明人： Brendan Larder , Sharon Kemp , Stuart Bloor , Ann Brophy
IPC分类号： C12P1934
CPC分类号： C12Q1/6837 , C12Q1/6858 , C12Q1/6869 , C12Q1/703 , C12Q2537/125 , C12Q2523/101 , C12Q2549/113
摘要： The present invention relates to a method of for mutation analysis of the HIV pol gene of HIV-1 virions comprising amplifying viral RNA or DNA via nested PCR using outer primers as represented in SEQ ID No: 1 and 2, amplifying said PCR product via nested PCR using a 5′ and 3′ primer chosen from the inner primers SEQ ID No: 3, 4, 5, and 6, and sequencing this secondary obtained PCR product using at least one sequencing primer chosen from any of SEQ ID No: 7 to 12 or variants thereof. In the alternative, at least one secondary sequencing primer may be used chosen from any of SEQ ID No: 13 to 24. The present invention also relates to kits for performing such a method as well as primers for performing the same.
摘要翻译： 本发明涉及HIV-1病毒粒子的HIV pol基因的突变分析方法，其包括使用SEQ ID No：1和2所示的外部引物通过巢式PCR扩增病毒RNA或DNA，通过嵌套扩增所述PCR产物 使用选自内部引物SEQ ID No：3,4,5和6的5'和3'引物进行PCR，并使用至少一种选自SEQ ID No：7至 12或其变体。 在替代方案中，可以使用从SEQ ID No：13至24中任一个选择的至少一个二次测序引物。本发明还涉及用于进行该方法的试剂盒以及用于进行该方法的引物。
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公开(公告)号：US07235387B2
公开(公告)日：2007-06-26
申请号：US10258151
申请日：2001-04-20
申请人： Brendan Larder , Sharon Kemp , Stuart Bloor , Ann Brophy
发明人： Brendan Larder , Sharon Kemp , Stuart Bloor , Ann Brophy
CPC分类号： C12Q1/6837 , C12Q1/6858 , C12Q1/6869 , C12Q1/703 , C12Q2537/125 , C12Q2523/101 , C12Q2549/113
摘要： The present invention relates to a method for mutation analysis of the HIV pol gene of HIV virions comprising amplifying virion RNA or DNA via nested PCR using outer primers as represented in SEQ ID No. 1 and 2, amplifying said PCR product via nested PCR using a 5′ and 3′ primer chosen from the inner primers SEQ ID No. 3, 4, 5, and 6, and sequencing this secondary obtained PCR product using at least one sequencing primer chosen from any of SEQ ID No. 7 to 12 or variants thereof. In the alternative, at least one secondary sequencing primer may be used chosen from any of SEQ ID No. 13 to 24. The benefit of the sequences present in the invention resides in the fact that, with the aid of the oligonucleotides, the sequences of all presently known HIV subtypes and all mutations of the pol gene presently known to yield resistance towards antiretroviral therapy can be determined. The present invention also relates to kits for performing such a method as well as primers for performing the same.
摘要翻译： 本发明涉及HIV病毒体的HIV pol基因的突变分析方法，其包括使用SEQ ID No.1和2所示的外部引物通过巢式PCR扩增病毒体RNA或DNA，通过巢式PCR扩增所述PCR产物，使用 选自内引物SEQ ID No.3,4,5和6的5'和3'引物，并使用至少一种选自SEQ ID No.7至12或任何变体的测序引物对该次级获得的PCR产物进行测序 其中。 在替代方案中，可以使用从SEQ ID No.13至24中任一个中选择的至少一个二次测序引物。存在于本发明中的序列的益处在于：借助于寡核苷酸， 目前已知所有目前已知的HIV亚型和目前已知对抗逆转录病毒治疗产生抗性的pol基因的所有突变可以被确定。 本发明还涉及用于进行这种方法的试剂盒以及用于进行该方法的引物。
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公开(公告)号：US20050064397A1
公开(公告)日：2005-03-24
申请号：US10935975
申请日：2004-09-08
申请人： Brendan Larder , Sharon Kemp , Stuart Bloor , Ann Brophy
发明人： Brendan Larder , Sharon Kemp , Stuart Bloor , Ann Brophy
CPC分类号： C12Q1/703
摘要： The present invention relates to a method of for mutation analysis of the HIV pol gene of HIV-1 virions comprising amplifying viral RNA or DNA via nested PCR using outer primers as represented in SEQ ID No: 1 and 2, amplifying said PCR product via nested PCR using a 5′ and 3′ primer chosen from the inner primers SEQ ID No: 3, 4, 5, and 6, and sequencing this secondary obtained PCR product using at least one sequencing primer chosen from any of SEQ ID No: 7 to 12 or variants thereof. In the alternative, at least one secondary sequencing primer may be used chosen from any of SEQ ID No: 13 to 24. The present invention also relates to kits for performing such a method as well as primers for performing the same.
摘要翻译： 本发明涉及HIV-1病毒粒子的HIV pol基因的突变分析方法，其包括使用SEQ ID No：1和2所示的外部引物通过巢式PCR扩增病毒RNA或DNA，通过嵌套扩增所述PCR产物 使用选自内部引物SEQ ID No：3,4,5和6的5'和3'引物进行PCR，并使用至少一种选自SEQ ID No：7至 12或其变体。 在替代方案中，可以使用从SEQ ID No：13至24中任一个选择的至少一个二次测序引物。本发明还涉及用于进行该方法的试剂盒以及用于进行该方法的引物。
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